Extraction process of high-purity phloridzin

Abstract

The invention discloses an extraction process of high-purity phloridzin. The process includes the steps of: (1) stock solution extraction: adding water that is 8 times the amount of a phloridzin-containing mashed raw material into the same, conducting soaking for 3h, performing heating to boiling, maintaining slight boiling for 2h to obtain an extracted solution; adding purified water that is 6 times the amount of residue into the same and performing heating to boiling, maintaining slight boiling for 2h to obtain a secondary extracted solution; merging the extracted solutions, carrying out cooling and filtering, and collecting the filtrate; (2) preliminary extraction of a finished product: subjecting the filtrate to adsorption by macroporous resin D101, controlling the column pass flow at 80L / H, using 20-50degree ethanol to conduct gradient elution, performing fractional collection on the outflow solution, carrying out vacuum concentration, standing and cooling, conducting crystal precipitation at 0-4DEG C, and carrying out high-speed centrifugal dehydration; and (3) finished product extraction: conducting pure water redissolution, vacuum concentration, recrystallization, high-speed centrifugal dehydration and drying, thus obtaining a finished product. The extraction process of high-purity phloridzin provided by the invention has easily available raw materials, not only solves the environmental problem, but also improves the product yield, and greatly reduces the cost, and has the advantages of simple reaction process, convenient operation and no pollution.

Description

technical field [0001] The invention relates to a process for extracting phlorizin, in particular to a process for extracting high-purity phlorizin, and belongs to the technical field of phlorizin extraction. Background technique [0002] Phloridizin is widely found in a variety of plants, mainly in Rosaceae plants such as apple trees, and its content is low in other plants. Phloridizin is a phenolic substance in apple trees. Research data show that root bark Glycosides account for 95% of the total phenolic content in apple trees, and the original phlorizin is extracted from the branches, bark and leaves of apple trees. At present, the methods for extracting phloridzin include resin adsorption method, polyamide chromatography column separation method and extraction method. Among them, resin adsorption method has large adsorption capacity, good selectivity, easy desorption, high mechanical strength, simple regeneration treatment, and adsorption. It is fast and does not pollu...

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Problems solved by technology

At present, the methods for extracting phlorizin include resin adsorption method, polyamide chromatographic column separation method and extraction method. Among them, the resin adsorption method has large adsorption capacity, good selectivity, easy desorption, high mechanical strength, simple regeneration treatment, and high adsorption capacity. The speed is fast, and it does not pollute the environment, but the toxicity of the pore-forming agent used in the preparation of the macroporous resin has always been the focus of controversy in this technology, which has become an obstacle to the separation of phlorizin by using the macroporous resin adsorption technology; Polyamide chromatography column separation method is simple to operate, good separation effect, safe, high loading capacity, and less non-specific adsorption. The disadvantage of this method is that methanol, which is moderately toxic to the human body, is used as the eluent during the elution process; Methanol has a great impact on the nervous system and blood system of the human body. It will produce toxic reactions when ingested through the digestive tract, respiratory tract or skin; the extraction method uses ethanol and acetic acid, which are less toxic to the human body, in the process of extracting phlorizin However, ethanol is used as the extraction agent in the purification process, and ethanol has a paralyzing effect on human nerves. Considering safety issues, it is difficult to apply it to medicines and foods; therefore, new studies are needed for the extraction of phlorizin. craft

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