Nucleic acid test card and application method thereof

A detection card, nucleic acid technology, applied in biochemical equipment and methods, bioreactors/fermenters for specific purposes, biochemical instruments, etc., can solve problems such as difficulties in doctors' diagnosis and symptomatic prescription

Inactive Publication Date: 2017-01-18
冯晓均
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In clinical cases, the same disease may be caused by many different pathogens, which makes it difficult for doctors to diagnose and prescribe medicines

Method used

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  • Nucleic acid test card and application method thereof
  • Nucleic acid test card and application method thereof

Examples

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example 1

[0023] Example 1: Simultaneous detection of various intestinal bacteria.

[0024] First prepare a nucleic acid test card that can be used for the simultaneous detection of multiple intestinal bacteria:

[0025] 1) Make a nucleic acid test card based on plastic material. 4 reagent wells, each preloaded with loop-mediated isothermal amplification reagents, including primers (used to amplify specific target genes of Escherichia coli, Salmonella, Shigella, and Vibrio parahaemolyticus), Bst polymerase, Tris hydrochloric acid buffer solution, deoxynucleotide triphosphate, calcium yellow-green fluorescent indicator; 4×5 reaction wells, each reaction well fixed a piece of nucleic acid collection test paper (such as Whatman FTA test paper), used for 3 samples, 1 negative control Simultaneous detection with 1 positive control;

[0026] 2) Seal the reagent wells and reaction wells with an adhesive sealing film, and store the nucleic acid detection card at -20°C for a long time.

[002...

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Abstract

The invention provides a nucleic acid test card and an application method thereof. The nucleic acid test card comprises a sealing film, a card body, a reagent hole, a nucleic acid amplification reagent, reaction holes, nucleic acid collection test paper, a nucleic acid purifying reagent for matching use, a washing buffer and an organic phase for preventing a solution from being volatized. The application method comprises the following steps: opening the sealing film, adding a pathogene sample into the reaction holes packaged with the nucleic acid collection test paper, automatically washing by the use of the nucleic acid purifying reagent and the washing buffer for several times, respectively adding the nucleic acid amplification reagent in the reagent hole into each corresponding reaction hole to carry out a nucleic acid amplification reaction under the protection of the organic phase for preventing a solution from being volatized, and carrying out qualitative and quantitative analysis on the reaction result through an optical inspection instrument for matching use. By the adoption of the test card and through a nucleic acid amplification reaction, simultaneous testing of a single sample or various pathogens or target genes in various samples is realized. Time consumption of the test is low, test accuracy and sensitivity are high. The test card is applicable in the fields of experiment research, clinical test and food safety testing, etc.

Description

technical field [0001] The invention relates to the field of nucleic acid analysis and detection, in particular to a nucleic acid detection card based on nucleic acid amplification reaction and its use method. Background technique [0002] The outbreak of SARS in most parts of China in 2003, the epidemic spread of bird flu in 2005 and the high-fatal Ebola epidemic in Africa in 2014, this series of infectious diseases caused by viruses tell us that in the history of human development, there has always been Threatened by the deadly virus. The spread and mutation of the virus have greatly exceeded human expectations. Timely and accurate diagnosis of the virus is of great significance for improving the survival rate of patients, controlling the spread of the epidemic, and effective disease treatment. [0003] The conventional detection method of pathogenic microorganisms adopts in vitro culture technology, but only about 1% of bacteria or viruses can be cultured, and the comple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/10C12M1/00
CPCC12Q1/6806
Inventor 冯晓均胡锐
Owner 冯晓均
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