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Basophilic Cupriavidus alkaliphilus strain used for highly efficiently removing cadmium pollution, and production method and application method of inoculant of strain

A technology for cadmium pollution and copper-loving bacteria, which is applied to the field of alkalophilic copper-loving bacteria for efficiently removing cadmium pollution, can solve the problem of high treatment cost, and achieve the effects of improving treatment effect, improving treatment efficiency and good resistance to heavy metals.

Inactive Publication Date: 2017-02-01
文山州广那高速公路投资建设开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional treatment technologies include chemical precipitation, electrolysis, ion exchange and membrane treatment, etc., and the cost of treatment is relatively high

Method used

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  • Basophilic Cupriavidus alkaliphilus strain used for highly efficiently removing cadmium pollution, and production method and application method of inoculant of strain
  • Basophilic Cupriavidus alkaliphilus strain used for highly efficiently removing cadmium pollution, and production method and application method of inoculant of strain
  • Basophilic Cupriavidus alkaliphilus strain used for highly efficiently removing cadmium pollution, and production method and application method of inoculant of strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: detection basis

[0033] Determination method of cadmium in sewage:

[0034] Determination of water quality cadmium dithizone spectrophotometric method (GB 7471-87 1987-08-01 implementation)

Embodiment 2

[0035] Embodiment 2: laboratory removes cadmium test

[0036] ① Strain medium preparation: 0.05% yeast powder, 0.1% peptone, 0.05% sodium chloride, 0.05% sucrose, adjust the pH value to 7.2, and sterilize at 121°C for 30 minutes. Solid medium: add 2% agar to the above medium.

[0037] ② Strain fermentation broth preparation: Inoculate 3 loops of the strain into 100ml medium, culture on a shaker for 48 hours, culture temperature 30°C, shaker speed 100 (rev / min).

[0038] ③Add cadmium nitrate to the fermentation broth produced by the above method, so that the cadmium concentration reaches 1000mg / L, 2000mg / L, 3000mg / L respectively, the control temperature is 30°C, the shaking table speed is 120r / min, and the treatment interval is 24h under the condition Samples were taken to measure the concentration change of cadmium in the supernatant. Sampling was stopped when the concentration of cadmium in the medium was lower than the detection limit.

[0039] ④Test results

[0040] The...

Embodiment 3

[0043] Embodiment 3 The concrete steps of product processing low cadmium polluted water of the present invention are as follows:

[0044] The product produced by the above method was added to 1 of the cadmium-contaminated water, and the concentration of hexavalent chromium contained in the 1 of the polluted water was 99.8mg / L. The volume ratio of the product to the polluted water is 3.5:100 (L / L), the pH value of the polluted water is adjusted to 7.0, the temperature of the polluted water is controlled at 30°C, and the stirring speed is 100r / min. concentration of ions. Sampling was stopped when the concentration of cadmium ions in the medium was lower than the detection limit.

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PUM

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Abstract

The invention discloses a Basophilic Cupriavidus alkaliphilus strain used for highly efficiently removing cadmium pollution. The strain is preserved in China General Microbiological Culture Collection Center on March 29, 2016 with the preservation number of 12319, and is named as Basophilic Cupriavidus alkaliphilus strain LYTTJ. The high-concentration cadmium-resistant strain has good heavy metal resistance, and the cadmium resisting concentration reaches 3000 mg / L. A result of experiments using the strain to treat cadmium polluted water shows that the cadmium removal rate of sewage with the cadmium concentration of 1000 mg / L or less is 100%, so discharge standards are completely reached, and the Basophilic Cupriavidus alkaliphilus strain has great social and economic values.

Description

technical field [0001] The invention relates to the technical field of microbial removal of cadmium pollution in water bodies, in particular to an alkaliphilic cupricophilus bacterium that can efficiently remove cadmium pollution, and a production method and an application method thereof. Background technique [0002] The statements in this section merely provide background information related to the present disclosure and may not constitute prior art. [0003] Cadmium is a silver-white lustrous metal. In 1817, Stromeyer in Germany separated brown powder from impure zinc oxide and heated it with charcoal to obtain cadmium. The main pollution source of cadmium is the wastewater discharged from electroplating, mining, smelting, dyestuff, battery and chemical industry. Cadmium is not an essential element for the human body. Cadmium in the human body is absorbed from the external environment after birth, and enters and accumulates in the body mainly through food, water and air...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20
CPCC02F3/34C02F2101/20C12N1/20C12N1/205C12R2001/01
Inventor 孙莹赵英郝东伟王新民
Owner 文山州广那高速公路投资建设开发有限公司