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Gypenosides, preparing method of gypenosides, and application of gypenosides in preparing anti-inflammatory drugs

A technology of Gynostemma saponin and Gynostemma pentaphyllum, applied in the directions of anti-inflammatory agents, drug combinations, antipyretics, etc., can solve the problems of insufficient extraction, purification and processing utilization of Gynostemma saponins.

Inactive Publication Date: 2017-02-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, Gynostemma pentaphyllum is more simply processed into Gynostemma pentaphyllum tea, and the extraction, purification and processing utilization of the active ingredient Gypenoside are far from enough, so the research on the extraction, purification and pharmacological activity of Gypenoside still needs further research In-depth discussion and comprehensive strengthening of the bioutilization of gypenosides is of great significance for the development of new drugs and the guidance of clinical medication

Method used

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  • Gypenosides, preparing method of gypenosides, and application of gypenosides in preparing anti-inflammatory drugs
  • Gypenosides, preparing method of gypenosides, and application of gypenosides in preparing anti-inflammatory drugs
  • Gypenosides, preparing method of gypenosides, and application of gypenosides in preparing anti-inflammatory drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 prepares Gypenoside (GPMS) from Gynostemma pentaphyllum

[0049] (1) After Gynostemma pentaphyllum Makino is pulverized with a pulverizer, pass through a 60-mesh sieve to obtain Gynostemma pentaphyllum coarse powder for subsequent use;

[0050] (2) Weigh 200 g of Gynostemma pentaphyllum powder, add 2000 mL of ethanol solution with a volume fraction of 75% in a 5000 mL round-bottomed flask, shake well, keep boiling at 100°C for 3 hours, stop heating, filter, and heat and reflux for 3 hours Once, the combined filtrates were centrifuged at 4000rpm for 10min, and the resulting supernatant was concentrated under reduced pressure with a rotary evaporator at 50°C to obtain the ethanol crude extract extract;

[0051] (3) Dissolve the Gynostemma ethanol crude extract extract with 1.5L of distilled water, add 0.5L of petroleum ether solvent, fully shake and mix, let stand for 40min, after the two-phase solvent is completely separated, suck out the petroleum ether pha...

Embodiment 2

[0052] Embodiment 2 prepares Gypenoside (GPMS) from Gynostemma pentaphyllum

[0053] (1) After Gynostemma pentaphyllum Makino is pulverized with a pulverizer, pass through a 60-mesh sieve to obtain Gynostemma pentaphyllum coarse powder for subsequent use;

[0054] (2) Weigh 200g of Gynostemma pentaphyllum powder, in a 5000mL round bottom flask, add 2000mL of ethanol solution with a volume fraction of 70%, shake well, keep at 100°C for a slight boiling extraction for 2h, stop heating, filter, heat and reflux for extraction for 3 Once, the combined filtrates were centrifuged at 3000 rpm for 8 min, and the resulting supernatant was concentrated under reduced pressure with a rotary evaporator at 55° C. to obtain the ethanol crude extract extract;

[0055] (3) Dissolve the ethanol crude extract of Gynostemma pentaphyllum ethanol with 1.8L of distilled water, add 0.6L of petroleum ether solvent, fully oscillate and mix, let stand for 30min, after the two-phase solvent is completely ...

Embodiment 3

[0056] Embodiment 3 prepares Gypenoside (GPMS) from Gynostemma pentaphyllum

[0057] (1) After Gynostemma pentaphyllum Makino is pulverized with a pulverizer, pass through a 60-mesh sieve to obtain Gynostemma pentaphyllum coarse powder for subsequent use;

[0058] (2) Weigh 200g of Gynostemma pentaphyllum powder, in a 5000mL round-bottomed flask, add 3000mL of 80% ethanol solution by volume fraction, shake well, keep at 95°C for a slight boiling extraction for 4h, stop heating, filter, heat and reflux for extraction 2 Once, the combined filtrates were centrifuged at 5000 rpm for 10 min, and the resulting supernatant was concentrated under reduced pressure with a rotary evaporator at 60° C. to obtain the ethanol crude extract extract;

[0059] (3) Dissolve the ethanol crude extract of Gynostemma pentaphyllum ethanol in 3L of distilled water, add 1L of petroleum ether solvent, fully oscillate and mix, and let stand for 60min. After the two-phase solvent is completely separated, ...

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Abstract

The invention belongs to the field of traditional Chinese medicine, and discloses a gypenosides, a preparing method of the gypenosides, and an application of the gypenosides in preparing anti-inflammatory drugs. The preparing method comprises the first step of carrying out smashing and sifting on the gynostemma pentaphyllum to obtain coarse gynostemma pentaphyllum powder; the second step of adopting ethanol solution to carry out heating and refluxing on the coarse gynostemma pentaphyllum powder to extract the coarse gynostemma pentaphyllum, conducting centrifugation on the extracting solution and then conducting concentration on liquid supernatant to obtain concentrated gynostemma pentaphyllum ethyl alcohol crude extract; the third step of using distilled water to dissolve the gynostemma pentaphyllum ethyl alcohol crude extract, adding an organic solvent to conduct extraction, containing pigment, adding chloroform or ethyl acetate into the water layer to continue the extraction and removing organic layer to obtain an aqueous solution; adding n-butyl alcohol to carry out extraction, collecting normal butyl alcohol phase, conducting concentration and drying through distillation to obtain the gypenosides. The obtained gypenosides have an obvious anti-inflammatory activity, and the dosage of the gypenosides is usually low due to the advantage of plant saponin, besides, under the same dosage, the toxic and side effects of the gypenosides are lower.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a gypenoside, a preparation method and an application in the preparation of anti-inflammatory drugs. Background technique [0002] Saponins, named for their foaming characteristics, are a class of glycoside compounds with important medical value. According to the structure of saponins produced after hydrolysis, they are divided into two categories: triterpene saponins and steroidal saponins. Saponins widely exist in nature, such as soybeans, bitter melon, jujube and other common foods. In addition, saponins are also the main active ingredients of traditional Chinese medicines recorded in some herbal works, such as ginseng, Panax notoginseng, Anemarrhena, polygala, licorice, yam, etc. were praised as "top-grade medicines" by the ancients because of their remarkable health effects. [0003] Inflammation is involved in the pathogenesis of many chronic diseas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/424A61P29/00
CPCA61K36/424A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 姜建国申春燕石嫚嫚
Owner SOUTH CHINA UNIV OF TECH
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