High-efficiency antibiotic wastewater treatment agent and preparation method and application thereof

A technology for antibiotic wastewater and high-efficiency degrading bacteria, which is applied in biological water/sewage treatment, biochemical equipment and methods, natural water treatment, etc. The preparation process is simple and feasible, easy to popularize and apply, and has the effect of low cost

Active Publication Date: 2017-02-22
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problems that the preparation and production cost of the existing antibiotic wastewater treatment bacteria agent is relatively high and the preparation process is complicated, thus limiting its application in the water treatment process. On...

Method used

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  • High-efficiency antibiotic wastewater treatment agent and preparation method and application thereof
  • High-efficiency antibiotic wastewater treatment agent and preparation method and application thereof
  • High-efficiency antibiotic wastewater treatment agent and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1. Strain Isolation and Identification

[0036] This example demonstrates a preparation method of bacterial agent for high-efficiency treatment of penicillin wastewater, and its process flow chart is as follows figure 1 As shown, the specific steps are:

[0037] (1) Collect 10mL of the mixed solution in the aeration tank of the biological sewage treatment plant, put it into a 50mL centrifuge tube, add glass beads with a diameter of 0.5mm, the filling ratio is 1%, and then place it on a vortex shaker for 10 minutes , fully break up the sludge, and then let it stand for 10 minutes.

[0038] (2) Take 1 mL of the supernatant after standing still for gradient dilution, and then spread it on a solid medium containing 2% (mass concentration) sodium sulfate, culture at 20°C for 24 hours, and pick a single colony to a new Streak culture on the flat plate, pick and isolate and purify repeatedly, and expand the single strain in the liquid medium corresponding to the solid medium...

Embodiment 2

[0047] 1. Strain Isolation and Identification

[0048] This example demonstrates a preparation method of bacterial agent for high-efficiency treatment of penicillin wastewater, and its process flow chart is as follows figure 1 As shown, the specific steps are:

[0049] (1) Collect 10mL of the mixed solution in the aeration tank of the biological sewage treatment plant, put it into a 50mL centrifuge tube, add glass beads with a diameter of 1.0mm, and the filling ratio is 2% (accounting for the volume ratio of the centrifuge tube), and then place Vortex on a vortex shaker for 20 minutes to fully break up the sludge, and then let it stand for 30 minutes.

[0050] (2) Take 1 mL of the supernatant after standing still for gradient dilution, and then spread it on a solid medium containing 2% (mass concentration) sodium chloride, culture at 25°C for 32 hours, and pick a single colony to Streak culture on a new plate, pick and isolate and purify repeatedly, and expand the single str...

Embodiment 3

[0059] 1. Strain Isolation and Identification

[0060] This example uses the above method to demonstrate the preparation process of a bacterial agent for efficient treatment of tetracycline wastewater, and its process flow chart is as follows figure 1 As shown, the specific steps are:

[0061] (1) Collect 10mL of the mixed solution in the aeration tank of the biological sewage treatment plant, put it into a 50mL centrifuge tube, add glass beads with a diameter of 0.2mm, the filling ratio is 3%, and then place it on a vortex shaker for 20 minutes , fully break up the sludge, and then let it stand for 30 minutes.

[0062] (2) Take 1 mL of the supernatant after standing still for gradient dilution, and then spread it on a solid medium containing 10% (mass concentration) sodium sulfate, culture at 25°C for 48 hours, and pick a single colony to a new Stretch culture on the flat plate, pick and isolate and purify repeatedly, and expand the single strain in the liquid medium corres...

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Abstract

The invention discloses a high-efficiency antibiotic wastewater treatment agent and a preparation method and application thereof. The high-efficiency treatment agent comprises the following steps that 1, mixed liquid in an aeration tank of a domestic sewage treatment plant is taken, a proper amount of glass beads is added, and then standing is performed; 2, supernate is taken, gradient dilution is performed, flat plate coating is conducted on a solid culture medium containing sodium chloride or sodium sulfate, and salt-tolerant strains are screened out; 3, reagent resistance of the screened salt-tolerant strains is analyzed; 4, the cultured strains having high reagent resistance are inoculated to a salt-containing liquid culture medium in an independent or mixed mode for culture, and the antibiotic high-efficiency degradable fungicide can be obtained. The preparation method is very simple, easy to operate and high in safety, no antibiotic is used in the fungicide production process, and the obtained fungicide can be used for high-efficiency antibiotic degradation and can be applied to high-efficiency antibiotic wastewater treatment.

Description

technical field [0001] The invention belongs to the technical field of wastewater treatment, and more specifically relates to a bacterial agent for efficient treatment of antibiotic wastewater and its preparation method and application. Background technique [0002] With the rapid development of my country's pharmaceutical industry, the discharge of pharmaceutical wastewater has become increasingly prominent. The annual discharge of antibiotic wastewater has reached hundreds of millions of cubic meters. Antibiotic wastewater usually contains high concentrations of organic pollutants, high salinity, and residual antibiotics and related products. , Such waste water is directly treated with aerobic biological treatment technology, and the energy consumption is very high. At the same time, due to the antibacterial effect of antibiotics, the biological treatment effect is also unstable. The conventional treatment process cannot achieve the standard discharge of antibiotic producti...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/22C02F103/34
CPCC02F3/34C02F2103/343C12N1/20C12N1/205C12R2001/22
Inventor 叶林刘美田陶文达张徐祥任洪强
Owner NANJING UNIV
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