Method for rapidly detecting histamine in meat product

Abstract

The invention discloses a method for rapidly detecting histamine in a meat product and relates to the technical field of histamine measurement. The method includes the specific detection steps of reagent preparation, sample pretreatment, preparation of control sample test fluid, preparation of to-be-tested sample test fluid and testing of a control sample and a to-be-tested sample. According to the step of testing of the control sample and the to-be-tested sample, a disease meat detector OK-BH10 is started, a histamine detection item is selected, then a cuvette containing the control sample test fluid is put in a first channel, a key of control measurement is clicked, then a cuvette containing the to-be-tested sample test fluid is put in the first channel, a key of sample measurement is clicked, and the detector can automatically display the histamine content. According to the testing method, the sample treatment process is simple, operation is simple and easy to carry out, the reagent use amount is low, the detection cost is greatly reduced, the detection sensitivity is high, the histamine matter can be fully detected, the detection time is short, and a large amount of time is saved.

Description

[0001] Technical field: [0002] The invention relates to a method for quickly detecting histamine in meat products, and belongs to the technical field of histamine determination. [0003] Background technique: [0004] Histamine (HA), as a kind of biological amine, is produced by microorganisms acting on its precursor amino acid-histidine. It is widely present in various foods and organisms, especially fermented foods (such as cheese, wine, and fermentation). Sausages, soy sauce, aquatic products and meat products, etc.). It is not only one of the important mediators of inflammatory response and immune damage, but also has an important regulatory role in response to inflammatory response. However, when the human body consumes excessive amounts of histamine, it can cause allergic reactions such as headache, nausea, palpitations, blood pressure changes, breathing disorders, and even life-threatening. [0005] The content of histamine in food is closely related to human health and affe...

AI Technical Summary

Problems solved by technology

[0007] At present, the existing detection methods have the following disadvantages: 1. The sample processing procedure of the fluorescence photometry method is complicated, and the operation requirements are relatively strict.

[0008] 2. ELISA has certain limitations, such as the inability to analyze multiple components at the same time, high selectivity to reagents, and a certain degree of cross-reaction to compounds with similar structures

And the test results are less stable

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