Method for detecting mold in food

Abstract

The invention discloses a method for detecting mold in food. The method includes the steps: weighing 1-5g of video samples to be detected by a standard weighing appliance; vibrating the samples by 20ml of 80% acetonitrile-water solution containing 0.1% of methanoic acid and extracting supernate; placing the solution into a centrifuge for centrifuging for 20-40 minutes at the speed of 1000-2000 revolutions per minute to obtain extracting solution A. After the food is pretreated by an improved QuEChERS method, 26 extracted mold toxins are simultaneously detected by super-efficient liquid chromatography tandem mass spectrometry. The samples are extracted twice by the acid-containing acetonitrile-water solution with different concentrations, the sample extracting solution is salted out, degreased, purified, concentrated and re-dissolved, the mold is detected by a multi-reaction monitoring mode of super-efficient liquid chromatography tandem mass spectrometry, detection efficiency is improved, detection cost is saved, sensitivity and adding standard recovery rate are high, repeatability is good, and the method can be expanded to detect other samples with high fat content.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of mold in food. Background technique [0002] Mycotoxins are a global problem. According to estimates by the FAO, about 5-7% of agricultural products such as grain and feed in the world are damaged by molds every year, and 25% of grains are contaminated by known mycotoxins, causing hundreds of billions of dollars in damage every year. dollar loss. In the 1990s, the loss caused by mycotoxin pollution in the Canadian feed industry was estimated to be more than US$1 billion, while that in the United States was as high as US$2.5 billion. billions of dollars in losses. [0003] Mycotoxins are secondary metabolites produced by some fungi such as Aspergillus, Fusarium, and Penicillium after they grow and mature. These toxins can be produced in multiple stages of grain harvesting, storage, processing, and transportation. There are more than 400 known mycotoxins, mainly includi...

AI Technical Summary

Problems solved by technology

The principle of enzyme-linked immunoassay is to use the combination of antibody and enzyme complex, and then detect through color development; thin-layer chromatography is based on the comparison of the ratio shift value (Rf) of the chromatogram obtained by the same method with a suitable control substance. The method of content determination, both of which are semi-quantitative methods, are mostly used for the preliminary screening of one or a few toxins; high-performance liquid chromatography uses a high-pressure infusion system to mix a single solvent with different polarities or different proportions The mixed solvent, buffer and other mobile phases are pumped into the chromatographic column equipped with stationary phase. After the components in the column are separated, they enter the detector for detection, so as to realize the analysis of the sample. This method is accurate in quantification, but for simultaneous When detecting multi-component mycotoxins and incomplete purification of the sample matrix, the target compound cannot achieve baseline separation on the chromatographic column, and the target compound cannot be accurately quantified