Antioxidant dipeptide from fructus perillae, method for preparing dipeptide and application thereof

An oxidized dipeptide and antioxidant peptide technology, applied in the field of food biology, can solve the problems of less research on protein development and utilization, and no report on active peptide pharmacology.

Active Publication Date: 2017-03-08
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, domestic and foreign researches on perilla seeds mainly focus on oil extraction and biological activity, while there are few researches on the devel

Method used

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  • Antioxidant dipeptide from fructus perillae, method for preparing dipeptide and application thereof
  • Antioxidant dipeptide from fructus perillae, method for preparing dipeptide and application thereof
  • Antioxidant dipeptide from fructus perillae, method for preparing dipeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1

[0028] The separation and purification of the antioxidant dipeptide of the present invention includes two steps of Sephadex G-25 gel filtration chromatography and reverse phase high performance liquid chromatography (RP-HPLC).

[0029] Preparation of crude perilla seed proteolysis product: firstly degrease the perilla seed with petroleum ether, then use the alkaline extraction and acid precipitation method to obtain the crude perilla seed protein, use alkaline protease to hydrolyze the perilla seed crude protein with alkali The best enzymolysis conditions for sex protease: pH 9.80, enzyme dosage 3000U / g, substrate concentration 3% (w / v), hydrolysis time 5.00h, boiling water bath for 10min inactivation, centrifugation at 12000rpm for 10min, take it The clear liquid is freeze-dried, which is the product of enzymatic hydrolysis of perilla seed protein;

[0030] Sephadex G-25 Gel Filtration Chromatography: The lyophilized powder of perilla seed enzymatic lysate is dis...

Example Embodiment

[0034] Example 2

[0035] The activity of the natural antioxidant dipeptide obtained in Example 1 was studied:

[0036] ABTS free radical scavenging effect: prepare 7 mmol / L ABTS with distilled water + Solution and 2.45 mmol / L potassium persulfate solution (before use must be placed at room temperature for 16 h), respectively, ABTS + Mix with potassium persulfate solution in a volume ratio of 1:1, and dilute the mixture to absorbance A with pH 7.4, 5 mmol / L phosphate buffer solution immediately before use 734 It is 0.70±0.02. Take 0.5 mL of samples of different concentrations and 0.5 mL of ABTS free radical solution, mix and stand for 10 minutes, and measure the absorbance at 734 nm. Use deionized water and reduced glutathione instead of samples as blank control and positive control, respectively. ABTS free radical scavenging activity is calculated according to the following formula (1):

[0037] (1)

[0038] In the formula, A 0 : Absorbance value of blank control group; A s : Abs...

Example Embodiment

[0056] Example 3:

[0057] The natural antioxidant dipeptide obtained in Example 1 was subjected to cytotoxicity and antioxidant research:

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Abstract

The invention discloses an antioxidant dipeptide from fructus perillae, a method for preparing the dipeptide and application thereof. A sequence of the antioxidant dipeptide is Phe-Tyr (PF). The antioxidant dipeptide, the method and the application have the advantages that as shown in in-vitro experiments, ABTS, hydroxyl free radicals and superoxide anions can be effectively removed by the antioxidant dipeptide, and the antioxidant dipeptide is high in reactive oxygen species free radical scavenging capacity (ORAC); peroxidation of linoleic acid and rat hepatic lipids can be effectively suppressed by the antioxidant dipeptide; as proved by cell experiments, the antioxidant dipeptide with low concentration is safe for cells, and obvious effects of protecting the cells from oxidative injury can be realized by the antioxidant dipeptide; obvious effects of inhibiting cancer cells can be realized when HepG-2 cells are treated by the antioxidant dipeptide with high concentration; the antioxidant dipeptide is simple in structure and high in antioxidant activity and can be used as an excellent substitute for existing artificially synthesized antioxidants, the antioxidant dipeptide and the method have important value in the aspects of development and application of novel antioxidant healthcare product and food additives, and the like.

Description

technical field [0001] The invention relates to a perilla seed natural antioxidant dipeptide, a preparation method thereof and an application in the fields of food and health care products, belonging to the field of food biotechnology. Background technique [0002] Perilla seed, also known as perilla seed, is the dry and mature fruit of perilla frutescens, an annual herb of the genus Perilla in the family Lamiaceae. Perilla is native to China. There are wild species and cultivated species in North China, South China, Central China, Southwest China and Taiwan Province. It is one of the first 60 medicinal and edible plants approved by the Ministry of Health of my country. Perilla, as a traditional Chinese medicine in my country, was first recorded in "Compendium of Materia Medica" by Li Shizhen, a medical sage in the Ming Dynasty. Perilla seeds are rich in oil. Due to the limitation of processing technology, perilla seeds are mainly used to extract edible oil in my country's ...

Claims

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Application Information

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IPC IPC(8): C07K5/065C12P21/06C07K1/20C07K1/16A23L33/18
CPCA23V2002/00C07K5/06078C12P21/06A23V2200/30A23V2200/308
Inventor 洪晶胡磊王惠敏孟春
Owner FUZHOU UNIVERSITY
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