Sesquiterpene synthase from eupatorium adenophorum spreng, gene, vector, engineering bacterium and application of sesquiterpene synthase

Abstract

The invention discloses sesquiterpene synthase from eupatorium adenophorum spreng, a gene, a vector, an engineering bacterium and application of the sesquiterpene synthase. Amino acid sequences of the sesquiterpene synthase are shown as SEQ ID NO.2. The sesquiterpene synthase, the gene, the vector, the engineering bacterium and the application have the advantages that catalysts come from the eupatorium adenophorum spreng which is a high-yield medicinal plant for beta-elemene, as proved by in-vitro activity, substrates FPP (Farnesyl pyrophosphate) can be catalyzed by the catalysts to generate the beta-elemene, and the final yield of the beta-elemene can ultimately reach 7.499 micro-grams / mL.

Description

[0001] (1) Technical field [0002] The invention relates to the preparation of β-elemene, in particular to a sesquiterpene synthase derived from Eupatorium adenophorum, a gene, a carrier, an engineering bacterium and an application thereof for preparing β-elemene. [0003] (2) Background technology [0004] Elemene (Elemene) is an active ingredient extracted from turmeric, a plant of Zingiberaceae, which has anti-cancer effects. It is a new class of sesquiterpene drugs independently developed in my country. In clinical application, the drug is highly effective and has few side effects; it can improve microcirculation and help chemotherapy and radiotherapy drugs enter cancer tissues to play a role; it has dual effects of selectively inhibiting tumor cell proliferation and improving immune function; it can also directly act on cell membrane, causing tumor cells to rupture and die. At present, elemene is being widely used in the treatment of malignant tumors such as lung cancer,...

AI Technical Summary

Problems solved by technology

Although the development and clinical application of elemene series of anti-tumor herbal medicines are in full swing, and the market share is also expanding, there are still many technical difficulties in the extraction and separation of elemene from the natural plant curcuma curcuma: first, the natural plant only contains Elemene, and its content is extremely low, accounting for about 1 / 1000 to 2 / 1000; at the same time, the content of elemene is easily affected by many factors such as variety, root quality, planting environment, etc.

Second, the components in the volatile oil are complex. Elemene is mixed with many sesquiterpenoids with similar structures. It must be first separated by precision fractionation, molecular distillation or supercritical carbon dioxide method, and then through thin-layer chromatography and column chromatography. , the pure product can only be obtained after multiple separations, so the extraction process of elemene requires high difficulty

Because Eupatorium adenophorum has strong adaptability, stress resistance and strong competitiveness, once it invades forest land, grassland and other places, it can quickly form a single dominant community, causing great harm to the ecological environment

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