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Quality control method of Dayuan decoction composition

A quality control method and composition technology, which are applied in important analysis fields, can solve the problems of few indicators, single quality control, as many as the original drink composition, etc., and achieve the effects of objective conclusion, convenient operation and control of preparation quality.

Inactive Publication Date: 2017-03-08
CHINA RESOURCES SANJIU MEDICAL & PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is to overcome that the traditional Chinese medicine compound preparation in the prior art basically only has one or two index components in content control, the quality control is single, the index is few, and it is difficult to fully reflect that there are many medicinal flavors, many components, Defects in the quality status of the Dayuan Drink composition with many targets, thereby providing a quality control method for the Dayuan Drink composition

Method used

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  • Quality control method of Dayuan decoction composition
  • Quality control method of Dayuan decoction composition
  • Quality control method of Dayuan decoction composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Embodiment 1. The preparation of Dayuan drink composition

[0092] The first Dayuan drink composition: Weigh the following decoction pieces by weight: 6g betel nut, 3g magnolia officinalis, 1.5g grass nut, 3g anemarrhena, 3g white peony root, 3g skullcap, 1g licorice, and place them in a 2L decoction casserole , add 400ml of water, soak for 30 minutes, cover, place the casserole on an electric heating plate, heat it to a boil (about 13 minutes) on a strong fire (voltage value of 220V), keep it on a slow fire (voltage value of about 170V) until the medicinal liquid is about 160ml (about 40 minutes), use two layers of medical gauze to filter out the medicine residue, let it cool, and get it.

[0093] The second Dayuan drink composition: take the following decoction pieces by weight: 1200g betel nut, 600g magnolia bark, 300g grass nut, 600g anemarrhena, 600g white peony root, 600g scutellaria baicalensis, 300g licorice; add 8 times the amount of water, decoct Boil for 1.0...

Embodiment 2

[0094] Embodiment 2. HPLC method is determined the content of arecoline in the composition of Dayuan drink

[0095] (1) Chromatographic conditions

[0096] Use strong cation exchange bonded silica gel as filler, chromatographic column: Phenomenex Luna SCX, 4.6*250mm, 5um, use acetonitrile-phosphoric acid solution (2→1000, adjust the pH value to 3.8 with concentrated ammonia test solution) (50:50) as Mobile phase; the detection wavelength is 215nm, the flow rate is 1.0ml / min, and the column temperature is 30°C. The number of theoretical plates should not be less than 3000 based on the peak of arecoline.

[0097] (2) Preparation of reference substance solution

[0098] Take an appropriate amount of arecoline hydrobromide, weigh it accurately, add acetonitrile-0.2% phosphoric acid solution (2→1000, adjust the pH value to about 3.8 with concentrated ammonia test solution) as the mobile phase (50:50) to make a solution containing 40 μg per 1 ml Solution, that is. (arecoline w...

Embodiment 3

[0108] Example 3. HPLC Determination of Mangiferin, Paeoniflorin, Baicalin and Glycyrrhizic Acid Contents in Dayuan Decoction Composition

[0109] (1) Chromatographic conditions: Octadecylsilane bonded silica gel as filler, chromatographic column: Agilent ZORBAX Eclipse Plus C18, 4.6*250mm, 5μm, acetonitrile as mobile phase A, 0.2% phosphoric acid solution as mobile phase B, Perform gradient elution as specified in the table below; flow rate: 0.8ml / min, column temperature: 30°C. The number of theoretical plates calculated based on the baicalin peak should not be less than 3000.

[0110] Mobile Phase Gradient Program

[0111]

[0112] Detection wavelength table

[0113]

[0114] (2) Preparation of reference substance solution

[0115] Take the appropriate amount of mangiferin reference substance, paeoniflorin reference substance, baicalin reference substance, and ammonium glycyrrhizinate reference substance, accurately weigh them, and add 70% methanol to make each 1m...

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Abstract

The invention discloses a quality control method of a Dayuan decoction composition, and belongs to the field of traditional Chinese medicine analysis. The method comprises the steps that high performance liquid chromatography is adopted for establishing a Dayuan decoction composition fingerprint spectrum, and according to the chromatographic conditions, an octadecyl silane bonded silica gel chromatographic column is adopted as a chromatographic column, the flow speed is 0.8 ml / min, the column temperature ranges from 25 DEG C to 35 DEG C, an ultraviolet-visible light detector is adopted as a detecting instrument, and the detecting wavelength of the fingerprint spectrum ranges from 215 nm to 240 nm; the number of theoretical plates is calculated according to a reference substance baicalin peak and should be not smaller than 3,000; a reference substance solution is a methanol solution of baicalin; a mobile phase is subjected to gradient eluting with an acetonitrile-0.1% phosphoric acid solution as a system according to the following eluting sequence, and the diagram is defined in the specification. According to the method, quality information of the Dayuan decoction composition is comprehensively reflected, and therefore the product quality can be controlled more comprehensively and effectively.

Description

technical field [0001] The invention belongs to the important analysis field, and in particular relates to a quality control method of the Dayuan drink composition. Background technique [0002] Traditional Chinese medicine decoction is the mainstream of clinical medicine, but because of decoction and inconvenient to carry, the quality of the decoction prepared by decoction often varies greatly depending on the person and the decoction utensils. The use of modern science and technology to develop traditional Chinese medicine preparations that are easy to carry and easy to take is not only the inheritance of traditional Chinese medicine, but also a better promotion of classic famous prescriptions. Clinical application. The preparation process of granules is relatively simple, easy to shape, easy to take and carry, and the form of taking is most consistent with traditional decoctions, and can maintain the characteristics of traditional decoctions to the greatest extent. There...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈世彬刘志刚陈周全谭沛张家曾马鹏岗王金艳刘志东
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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