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A method for extracting α-ketoglutarate from microbial fermentation liquid or enzyme conversion liquid

A technology of microbial fermentation broth and ketoglutaric acid, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of difficult separation and extraction methods and the inability to effectively separate pyruvate by-products, etc., and achieve simple and efficient process operation , Large industrial application potential, high economic value effect

Active Publication Date: 2019-03-05
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when the fermentation broth contains analogues of α-KG, such as pyruvate, which has similar physical and chemical properties to ketoglutaric acid, there are certain difficulties in traditional separation and extraction methods, for example, resin adsorption mainly based on molecular size and charge Analysis cannot effectively separate the pyruvate by-product

Method used

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  • A method for extracting α-ketoglutarate from microbial fermentation liquid or enzyme conversion liquid
  • A method for extracting α-ketoglutarate from microbial fermentation liquid or enzyme conversion liquid
  • A method for extracting α-ketoglutarate from microbial fermentation liquid or enzyme conversion liquid

Examples

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Effect test

Embodiment 1

[0044] Carry out the extraction of α-ketoglutaric acid as follows:

[0045] (1) Centrifugal filtration: pump the microbial fermentation broth containing α-KG into a centrifuge, and centrifuge to remove bacteria and other visible solids. Centrifugal conditions: room temperature, speed 3000r / min.

[0046] (2) Membrane filtration: Ultrafiltration is used to remove impurities, and the supernatant after centrifugation to remove bacteria and other visible solids is treated by ultrafiltration to remove bacterial fragments, pigments, and lipids in microbial fermentation broth or enzyme conversion fluid. Macromolecular substances such as substances, proteins and polysaccharides, and the filtered residue is washed with a small amount of water to improve the yield of the ultrafiltration process. The ultrafiltration membrane module used is a tubular membrane module. The membrane material is a polyethylene membrane with a molecular weight cut-off of 500-3000 Da. Ultrafiltration conditions...

Embodiment 2

[0055] The embodiment is the same as that in Example 1, the difference is that ion exchange resin is not used for impurity removal after concentration and before acidification.

[0056] The content of the prepared α-ketoglutaric acid was detected, and the purity was 98.1%, and the yield was 83.8%.

Embodiment 3

[0058] On the basis of Example 1, the membrane filtration step before concentration was omitted. The content of the prepared α-ketoglutaric acid was detected, and the purity was 95.3%, and the yield was 80.4%.

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Abstract

The invention discloses a method for extracting alpha-oxpentanedioic acid from a microbial fermentation liquid or an enzyme conversion liquid, and belongs to the technical field of biological separation extraction. The method comprises the following steps: carrying out centrifuge to remove thalli and other visible solids, carrying out ultrafiltration to remove macromolecular impurities, carrying out reduced pressure evaporation concentration, carrying out extraction with ethyl acetate or butyl acetate or another water-soluble organic solvent, carrying out evaporation, cooling and crystallization to obtain crude alpha-oxpentanedioic acid, washing the crude alpha-oxpentanedioic acid with ethyl acetate or butyl acetate or the another water-soluble organic solvent, and carrying out centrifuge, drying and crushing to obtain qualified final alpha-oxpentanedioic acid. The method has the advantages of simplicity and high efficiency in operation, low cost and high product purity, and is suitable for industrially producing alpha-oxpentanedioic acid.

Description

technical field [0001] The invention relates to a method for extracting α-ketoglutaric acid from microbial fermentation liquid or enzyme conversion liquid, and belongs to the technical field of biological separation and extraction. Background technique [0002] α-Ketoglutarate (α-KG) is a key intermediate metabolite in the tricarboxylic acid cycle and the main precursor in the metabolism of amino acids and proteins, and is widely used in food, medicine, agriculture and cosmetics. [0003] At present, there are mainly two ways of synthesizing α-KG: chemical method and biosynthetic method. The biosynthesis method mainly uses microbial fermentation to convert cheap carbon source substances or enzymatically converts other substances into α-KG. Traditional organic acid extraction methods mainly include resin adsorption analysis (itaconic acid, kojic acid, 2-keto-L-gulonic acid extraction) and calcium salt precipitation method (lactic acid, citric acid extraction). However, when...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/42C07C59/347
CPCC07C51/42C07C59/347
Inventor 周景文陈坚曾伟主张海林堵国成方芳刘松
Owner JIANGNAN UNIV