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Insect probiotics and feed additive

A technology for insect probiotics and feed additives, applied in animal feed, animal feed, bacteria and other directions, can solve the problems of the implementation effect of SIT technology affecting the quality of insect feeding, low mating ability, weak flying ability, etc., to enhance the physiological function of insects, The effect of improving the survival rate and increasing the pupa weight

Active Publication Date: 2017-03-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, SIT technology also has certain shortcomings. Compared with wild insect populations, artificially raised sterile males have some problems: compared with wild male populations, they have high mortality, long life history, low mating ability, and low production capacity. Fewer eggs, weak flying ability, etc.
The above problems have seriously affected the quality of insect breeding and the implementation effect of SIT technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The acquisition of embodiment 1 bacterial strain

[0020] (1) Isolation of insect intestinal microorganisms: pick adults or larvae of herbivorous insects such as Bactrocera citrus, Bactrocera citrus, beetles, beetles, termites, Tenebrio molitor, and soldier flies, and disinfect the surface of the insects To eliminate the influence of foreign microorganisms, dissect out the complete intestinal tract and put it into a sterile homogenizer filled with 300ul to 500ul sterile water, carefully dilute it after sufficient grinding, and apply NA plates to screen for cultivable bacteria.

[0021] (2) Identification of intestinal microorganisms of insects: single colony was picked by NA plate culture, inoculated in LB liquid medium, and incubated at 28-37° C. for 12-48 hours at a constant temperature. Using microbiology and molecular biology techniques to identify the types of strains, through the identification of Sphingobacterium, Fibrobacterium, Pseudomonas alcaligenes, Klebsiel...

Embodiment 2

[0026] The preparation of embodiment 2 insect probiotic feed additives

[0027] (1) Inoculate the preserved Klebsiella oxytoca BD177 and Citrobacter cusvii BD195 at a volume ratio of 1:1 in 100ml LB liquid medium with a total of 5% (v / v) inoculated amount , 30°C, 150r / min shaker culture, using a micro-spectrophotometer to measure the OD value of the bacterial solution at 600nm to 1.

[0028] LB medium: tryptone (nitrogen source) 1g, yeast powder (nitrogen source) 0.5g, NaCl 1g, 90mL deionized water, adjust the pH value to 7.4, adjust the volume to 100mL, and sterilize at 121°C for 15-20min.

[0029] (2) Take 0.3g of multivitamins, 0.2g of compound trace elements, and 1.5g of ascorbic acid and dissolve them in the above bacterial liquid, then fully mix them with 18g of wheat bran to make a probiotic feed additive, and add deionized water to 100g.

[0030] The effective viable count of the probiotics in the feed additive is 10 8 ~10 9 indivual.

[0031] The multivitamin cons...

Embodiment 3

[0032] The promoting ability of embodiment 3 insect probiotic feed additives to insect growth, reproduction

[0033] The fruit fly larvae used in the examples are fed with artificial feed: 7.5g of yeast powder, 50g of sucrose, 107g of wheat bran, 0.6g of multivitamin tablets, 3g of ascorbic acid, 300ml of sterile water, and the treatment group added 100g of Example 2 on this basis. For the prepared insect probiotic feed additive, the control group was added with a probiotic carrier (80ml sterile water+20g wheat bran), mixed and stirred evenly for use.

[0034] Add the lured fruit fly eggs into the feed, about 300 eggs per group. Feeding conditions: 28±2°C, humidity 70±5%, photoperiod 12h:12h. After 10 days of rearing, the larvae were transferred to the sand treated at 120°C for pupation. Detect the pupal weight, eclosion rate, and egg production of the insects in the treatment group and the control group. The result is as follows:

[0035] Table 1. Effects of probiotics on...

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PUM

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Abstract

The invention discloses two insect probiotics. The insect probiotics are separated from symbiotic bacteria in the intestinal tracts of fruit flies insects, are preserved in China Center for Type Culture Collection, and are Klebsiella oxytoca BD177 with the preservation number of CCTCCNO:M2015621 and Citrobacter koseri with the preservation number of CCTCCNO:M2015622 respectively. The invention also discloses a fruit flies insect feed additive prepared from the probiotics. The additive can improve the growing and breeding ability of the fruit flies insects, enhance the physiologic functions of the fruit flies insects and improve the irradiation damage restoration ability and the environment adaptation ability in order to improve the insect breeding efficiency and the insect sterile technology enforcement quality.

Description

technical field [0001] The invention belongs to the technical field of insect rearing technology and insect sterile biological control, and in particular relates to insect probiotics and an insect feed additive containing the probiotics. Background technique [0002] After the adult fruit fly insects mature, they insert the ovipositor under the epidermis of the host fruit and lay eggs inside the host fruit. The larvae hatch in the fruit, and then sneak into the fruit to cause damage, causing fruit borers, fruit drop, and rot. Fruits, etc., affect the fruit yield and quality, and even completely lose their edible value when serious, causing extremely serious economic losses. [0003] The use of sterile insect technique (sterile insect technique, SIT) can effectively control the population of pests in the area so as to achieve the purpose of prevention and control. SIT is to continuously release sterile male insects after a large number of target pests are raised and irradiate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K20/121A23K20/174A23K20/20A23K20/26C12R1/22C12R1/01
CPCC12N1/205C12R2001/01C12R2001/22
Inventor 张宏宇蔡朝辉王珊珊
Owner HUAZHONG AGRI UNIV
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