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A kind of antiviral biological agent prepared by using lentinan and its production process

A technology for biological preparations and lentinan, applied in the directions of antiviral agents, microorganisms, microorganism-based methods, etc., can solve the problems of high requirements on reaction conditions, low antiviral activity, and unsatisfactory disease resistance effects, and achieves a simple process. , The effect of preventing viral diseases and treating avian viral diseases

Active Publication Date: 2020-04-07
福建圣维生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the above-mentioned method has problems such as relatively high requirements for reaction conditions, inhomogeneous reaction, and low degree of substitution of the product, resulting in low antiviral activity. In practical application, the anti-disease effect is not ideal.
[0005] The prior art has the following defects: the antiviral activity of lentinan and lentinan derivatives is relatively low

Method used

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  • A kind of antiviral biological agent prepared by using lentinan and its production process
  • A kind of antiviral biological agent prepared by using lentinan and its production process
  • A kind of antiviral biological agent prepared by using lentinan and its production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 A process for preparing antiviral biological preparations using lentinan

[0029] Step 1 Bacillus subtilis activation

[0030] Take 1 tube of Bacillus subtilis lyophilized powder, add the lyophilized powder to 1ml YPD liquid medium prepared in advance, and shake to disperse;

[0031] The washed bacterial solution was streaked on the YPD solid medium, and placed in a 37°C incubator for 24 hours;

[0032] The components of the YPD liquid medium are as follows: glucose 20 g / L, yeast powder 10 g / L, peptone 20 g / L, and deionized water to make up the balance.

[0033] The components of the YPD solid medium are as follows: glucose 20 g / L, yeast powder 10 g / L, peptone 20 g / L, agar 20 g / L, and deionized water to make up the balance.

[0034] Step 2 seed liquid culture

[0035] Using lentinan to prepare a seed medium, and using the strain activated in step 1 to carry out fermentation and cultivation;

[0036] The components of the seed medium include: glucose 15g / L,...

Embodiment 2

[0060] Example 2 Carbon source single factor analysis experiment of fermentation medium

[0061] Adopt the method of Example 1 to prepare biological preparations, only change the carbon source of the fermentation medium in the fermentation step to carry out Examples 2-5; the carbon source of the fermentation medium used in Example 1 is 15g / L lentinan+5 g / L glucose

[0062] The carbon source of the fermentation medium that embodiment 2-5 adopts is shown in Table 2;

[0063] The carbon source of the fermentation medium that table 2 embodiment 2-5 adopts

[0064]

[0065] After testing, the antiviral activity of the biological preparations prepared in Examples 2-5 is shown in Table 3;

[0066] Table 3 The antiviral activity of the biological preparation prepared in Example 2-5

[0067]

[0068] It can be seen from Tables 2 and 3 that the carbon source of the fermentation medium has a great influence on the antiviral activity of the biological preparation, and the carbon ...

Embodiment 6

[0069] Nitrogen source single factor analysis experiment of embodiment 6 fermentation medium

[0070] Adopt the method of Example 1 to prepare biological preparations, only change the nitrogen source of the fermentation medium in the fermentation step to carry out Examples 6-10; the nitrogen source of the fermentation medium in Example 1 is: 12 g / L peptone+8 g / L urea;

[0071] The nitrogen source of the fermentation medium that embodiment 6-10 adopts is shown in Table 4;

[0072] The nitrogen source of the fermentation medium that table 4 embodiment 6-10 adopts

[0073]

[0074] After testing, the antiviral activity of the biological preparations prepared in Examples 6-10 is shown in Table 5;

[0075] The antiviral activity of the biological preparation prepared in table 5 embodiment 6-10

[0076]

[0077] It can be known from Table 4 and 5 that the nitrogen source of the fermentation medium is preferably 12 g / L peptone+8 g / L cottonseed meal.

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Abstract

The invention provides an antiviral biological preparation prepared from lentinan and a production process of the antiviral biological preparation. The antiviral activity of the biological preparation is 2.65*104IU / ml-9.24*104IU / ml. The production process comprises the following steps: activating bacillus subtilis, cultivating a seed solution and conducting fermentation so as to obtain the biological preparation. In the step of conducting fermentation, an adopted fermentation medium consists of the following components: 5-15g / L of the lentinan, 2-8g / L of sucrose, 6-10g / L of cotton seed meal, 9-15g / L of peptone, 0.1-0.5g / L of dipotassium phosphate, 0.1-0.5g / L of sodium chloride, 0.1-0.7g / L of anhydrous calcium chloride and the balance of de-ionized water. Thebiological preparation provided by the invention improves the antiviral activity by 1305-4552 times in comparison with that of the lentinan, and the biological preparation is good in stability.

Description

technical field [0001] The invention relates to a process for preparing an antiviral biological agent by using lentinan, in particular to an antiviral biological agent prepared by using lentinan and a production process thereof, belonging to the technical field of biological fermentation engineering. Background technique [0002] Poultry viral infectious diseases are one of the important factors restricting the development of poultry breeding industry, such as avian influenza and Newcastle disease, which cause huge economic losses to the poultry industry. At present, the chemical drugs used in poultry virus diseases at home and abroad are mainly amantadine and ribavirin preparations, the clinical curative effect is not exact, and there are toxic and side effects such as residues and affecting the recovery of laying rate of laying poultry. The industry has begun to pay attention to biological products that are green, residue-free, and non-toxic and side effects as new drugs. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04A61K31/715A61P31/12C12R1/125
CPCA61K31/715C12P19/04
Inventor 郭海岩刘刚王茂超王兴业韩国英刘东东郝利利郭芹王红军
Owner 福建圣维生物科技有限公司
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