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P. fluorescens strain and application thereof

A technology of Pseudomonas fluorescens and strains, applied in the direction of bacteria, biological water/sewage treatment, microorganisms, etc., to achieve the effect of simple cultivation method, good degradation effect, and not easy to mutate

Active Publication Date: 2017-05-24
HONGYUN HONGHE TOBACCO (GRP) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no literature report on the degradation of metalaxyl pesticide residues by Pseudomonas fluorescens (P.fluorescens)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The cultivation of thalline: pick the mycelia of Pseudomonas fluorescens (P.fluorescens) with an inoculation needle and inoculate it into the above-mentioned strain activation medium plate, and cultivate it for 4 days in a 28°C incubator at a temperature, and the bacterial strain is rod-shaped , 0.3μm~0.8μm×1.0μm~1.1μm, Gram staining negative, no spores, unipolar flagella, able to move; scrape the bacteria with a scalpel, inoculate into 500ml container under 121℃, 0.1MPa The culture solution was autoclaved for 20 minutes in an Erlenmeyer flask; then placed at 30°C with a rotation speed of 240rmp / min, and shaken for 4 days on a shaking table to obtain a mixed bacterial agent of mycelium and bacterial solution.

Embodiment 2

[0033] Supplement the degradation of metalaxyl residues in the carbon source solid medium: when the strain activation medium is sterilized and cooled to 40°C, mix in metalaxyl pesticide that has been sterilized by ultraviolet light for 30 minutes at a concentration of 5mg / L, and mix well Finally, pour it into a petri dish with a diameter of 60ml, with 3mL of culture medium per dish, and the culture medium is pink. The mycelium cultivated in Example 1 is punched into small pieces along the edge of the colony with a puncher with a diameter of 6 mm; after the culture medium mixed with metalaxyl pesticide is condensed, the small piece of mycelium is inserted, and the mycelium is close to the bottom medium surface. Placed in a 30°C incubator for 10 days, the pink color in the medium has faded, the molecular structure of metalaxyl pesticide has been degraded, and the medium is creamy translucent.

Embodiment 3

[0035] Degradation of high-concentration metalaxyl pesticide residues by degrading bacteria:

[0036]1) In 100ml of the culture solution cultivated according to claim 3, add 5mg / L of metalaxyl pesticide, at 30°C, with a rotating speed of 240rmp / min, shake the culture on a shaking table, add equal concentration of Metalaxyl pesticide was used as a control group;

[0037] 2) Take a sample every 12 hours, take 3ml of the sample and place it in a 50mL plastic centrifuge tube, add 10mL of acetonitrile, shake on the oscillator for 30min, centrifuge at 3000rmp / min, take out the supernatant, repeat 3 times, and combine the supernatant , after extracting the metalaxyl pesticide residue in the culture solution, purify it through a Florisil solid-phase extraction column (specification: 500mg3ml) in a solid-phase extraction device, concentrate on a rotary evaporator (below 40°C), and store it at -20°C Freeze-drying, then use a small amount of acetonitrile to dissolve and extract the pest...

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PUM

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Abstract

The invention discloses a P. fluorescens strain and application thereof. The strain is named P. fluorescens, preserved in CGMCC (China General Microbiological Culture Collection Center) of CCCCM (China Committee of Culture Collection for Microorganisms) on September 2, 2015, with the preservation number of PFpE1501. The P. fluorescens strain has industrial application prospect in biodegradation of pesticide residues in water and residual metalaxyl on surface.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a Pseudomonas fluorescens strain and application thereof. Background technique [0002] Chemical pesticides have the advantages of wide application range, many control objects, low production cost, good control effect and high economic benefit. Their wide application has greatly promoted the development of modern agriculture and improved the labor efficiency and mechanization degree of agricultural production. However, it is unavoidable that a large number of pesticides are used to kill insect natural enemies and beneficial microorganisms, make harmful organisms develop resistance, cause human and animal poisoning, and easily cause phytotoxicity to plants. Some of the chemical pesticides entering the environment can be exposed to sunlight, The action of microorganisms in the soil will gradually degrade and fail; the other part will eventually enter the huma...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/39A62D101/04A62D101/26A62D101/28C02F101/38C02F101/34
CPCA62D3/02C12N1/20C02F3/34C02F2101/34C02F2101/38C02F2101/306A62D2101/28A62D2101/26A62D2101/04C12R2001/39C12N1/205
Inventor 饶智张春花张静李伟罗云单治国蒋智林成文章崔宇翔满红平程昌新朱海滨董石飞杨义李枝桦程倩
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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