Method for rapidly detecting benzoic acid, sorbic acid, sodium saccharin and acesulfame potassium

Abstract

The invention relates to the technical field of food detection and particularly relates to a method for rapidly detecting benzoic acid, sorbic acid, sodium saccharin and acesulfame potassium. The method comprises the following steps: creating chromatographic conditions as follows: a chromatographic column is C18 150 mm * 4.6 mm * 5 [mu]m or C18 250 mm * 4.6 mm * 5 [mu]m, the mobile phase is that methyl alcohol plus 0.02 mol / L ammonium acetate equals to (10-15) plus (85-90), the flow velocity is 0.6-1.0 mL / min, the column temperature is 30+ / -5 DEG C, the detected wave lengths of benzoic acid, sorbic acid and sodium saccharin are 230 nm, the detected wave length of acesulfame potassium is 214 nm, and the injection volume is 10 [mu]L; accurately weighing 5.0000 g of a sample in a volumetric flask of 50 mL, adding 20 mL of I-grade water to dissolve, evenly mixing through vortex, implementing ultrasonic extraction for 30 minutes, adding 5 mL of potassium ferrocyanide solution and zinc acetate solution, implementing vortex precipitation for 3 minutes, metering the volume through the I-grade water, evenly mixing through vortex, processing through a water film of 0.22 mum, injecting the sample, preparing and mixing a standard solution, and drawing a standard solution curve. According to the method provided by the invention, the samples of various substrates are extracted together through the same extracting method, data of the four substances are acquired and analyzed at the same time, so that the working efficiency is greatly improved, and the experiment cost and the time are reduced and shortened.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to detection methods for benzoic acid, sorbic acid, saccharin sodium and acesulfame potassium. Background technique [0002] Food additives play a very important role in improving food quality, color, aroma, and taste. With the development of the food industry, their applications are becoming more and more extensive. However, most food additives are harmful to the human body. It may even cause cancer after being used in large quantities or beyond the range, so it is particularly important to control the safety and quality of various foods. GB 2760 "National Food Safety Standard Food Additive Use Standard" clearly stipulates the types and scope of use of food additives. Preservatives and sweeteners are relatively common food additives, while benzoic acid, sorbic acid, sodium saccharin, and acesulfame Potassium (acesulfame potassium) is the more common type of preservative and...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to overcome the above-mentioned defects that the detection of benzoic acid, sorbic acid, sodium saccharin and acesulfame potassium in the food existing in the prior art cannot be carried out simultaneously and samples of different substrates need to be processed with different pretreatment methods, and provide A method for simultaneous detection of benzoic acid, sorbic acid, sodium saccharin and acesulfame potassium. The same pretreatment method can be used for different food matrices. At the same time, the data of the four substances are collected and analyzed at the same time after the sample extract is put on the machine, so that Ability to quickly and accurately obtain analytical results

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