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A kind of Bacillus suffolus and its application in remediation of pollutants in open sea areas

A technology for bacillus saffordii and pollutants, which is applied to bacillus saffordii and its application field in the restoration of pollutants in open sea areas, and can solve the problems of few demonstrations and projects, lack of microbial restoration methods and applications of sediment pollution in open sea areas, etc. , to reduce the workload, ensure the delivery effect and repair effect, and increase the effect of pollutant degradation.

Active Publication Date: 2020-06-23
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, microbial remediation of oil pollution in marine sediments is mostly at the level of theoretical research and small-scale laboratory simulation of marine environment experiments, and there are few demonstrations and projects of large-scale microbial remediation of oil pollution in offshore sediments.
On the one hand, due to the special marine environment, offshore construction has strict requirements on weather, sea conditions, construction vessels and offshore operating experience. Now most of the organic pollution in the ocean, especially oil pollution remediation, engineering demonstrations are mainly concentrated in tidal flat areas, lacking open sea areas Microbial remediation method and application of sediment pollution

Method used

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  • A kind of Bacillus suffolus and its application in remediation of pollutants in open sea areas
  • A kind of Bacillus suffolus and its application in remediation of pollutants in open sea areas
  • A kind of Bacillus suffolus and its application in remediation of pollutants in open sea areas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Screening and combination of efficient petroleum degrading bacteria

[0037] Using the microorganisms in the sediments of the Yantai sea area as the source of highly efficient oil-degrading bacteria, the indigenous strains with good degradation effects on oil pollution were screened, and the bacterial liquid was mixed according to the ratio of the maximum degradation effect, adsorbed on the surface of zeolite, and wrapped with a coating agent, and finally Carry out subcontracting and release, and complete the bioremediation of oil-polluted sediments in designated sea areas of the Bohai Sea.

[0038] (1) Strain screening: Weigh 2g of petroleum-contaminated seawater sediment samples and add them to Erlenmeyer flasks filled with seawater culture medium and freshwater culture medium respectively, carry out shaking culture on a constant temperature shaker at 30°C and 180r / min for 3 days, and then pipette Insert 1 mL of the culture solution into the screening medium...

Embodiment 2

[0051] Example 2 Sea Area Sediment Oil Contaminated Microbial Restoration

[0052] Remediation of oil pollution in sediments in the Bohai Sea.

[0053] (1) Restoration strains: Acinetobacter calcoacetate, Pseudomonas putida and Bacillus shamford.

[0054] (2) Fermentation and mixing of bacterial strains: the three bacterial strains are respectively subjected to large-scale fermentation, and the respective concentration of the bacterial liquid after fermentation is 2-3OD. After fermentation, Acinetobacter calcoaceticus, Pseudomonas putida, and Bacillus spp. were mixed according to the ratio of the number of colonies of the strains to 3:2:4, and the total amount of bacteria in the mixed solution was about 1×10 9 CFU / ml, mix and store in a refrigerator at 4 degrees Celsius.

[0055] (3) Adsorption and packaging of bacterial liquid: the mixed bacterial liquid is mixed and adsorbed according to the mass ratio of bacterial liquid and zeolite at 2:1. The adsorption time is 2 hours....

Embodiment 3

[0067] Embodiment 3 Bacillus shamfu bacillus degrades the method for petroleum hydrocarbons

[0068] (1) Microbial strain: Bacillus suffolus (Bb);

[0069] (2) Seed culture: select a ring of colony from the plate and insert it into LB medium, shake the bottle with a liquid volume of 30%, the culture temperature is 28-30°C, the culture speed is 180-200 rpm, and the culture time is 10-16h. Centrifuge at 13,000rpm for 5min to collect the bacteria;

[0070] (3) Bb degrades petroleum hydrocarbons: the Bb thallus collected in step (2) is inoculated in the inorganic salt peptone liquid medium containing 2g / L petroleum hydrocarbons according to the initial concentration of 1 × 108 and cultivated. The inorganic salt peptone liquid medium containing petroleum hydrocarbon was used as a control. After 7 days, the experimental group and the control group were taken, and the remaining petroleum hydrocarbons were extracted with petroleum ether. After 14 days, the experimental group and the...

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Abstract

The invention relates to the field of water environment treatment by using microorganisms, in particular to Bacillus suffolus and its application. Bacillus suffolus was deposited on September 28, 2016 in the General Microbiology Center of China Committee for the Collection of Microorganisms, and its preservation number is: CGMCC No.13059. The method of the present invention obtains native degraded bacterial strains by screening, loads, flocculates, and embeds the obtained bacterial strains, and then applies the sea area for restoration. Using the bacterial agent of the present invention for restoration can ensure that the repaired strains reach the designated seabed sediments to the greatest extent, and can ensure that the strains The restoration effect; the bacterial agent does not need complicated equipment when it is put in, and can guarantee the effect of putting in and repairing effect.

Description

technical field [0001] The invention relates to the field of water environment treatment by using microorganisms, in particular to bacillus suffolus and its application in repairing pollutants in open sea areas. Background technique [0002] The problem of offshore oil spill and oil pollution has always been an important issue affecting the marine environment and marine ecology. At present, the emergency treatment of marine oil pollution, especially sudden oil spill accidents, is mainly aimed at the ocean surface seawater in the early stage of oil spill. Emergency disposal by means of physical interception, recycling, and chemical dispersants. However, there are no good physical and chemical treatment methods for seabed oil spills or oil pollution that sinks into seabed sediments with water movement. Environmental self-purification of oil-degrading microorganisms in sediments and artificial introduction of oil-degrading microorganisms are currently the solution to marine sed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/01C12R1/40
CPCC02F3/34C02F2103/007C12N1/20C12N1/205C12R2001/07
Inventor 王巧宁陈令新付龙文孙西艳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI