Chromosome culture medium for quickly harvesting metaphase cells and application
A culture medium and chromosome technology, applied in the fields of cell biology and human medical genetics, can solve the problems of time-consuming, time-consuming, and uneven quality of diagnosis results, so as to facilitate karyotype analysis and clinical diagnosis, and shorten the time required for diagnosis. The effect of taking time and shortening the processing time
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Embodiment 1
[0021] Example 1 The preparation of a chromosomal medium for rapidly harvesting metaphase cells comprises the following steps:
[0022] (1) Prepare penicillin sodium and streptomycin sulfate according to the following method
[0023] Preparation of Penicillin Sodium Injection:
[0024] Penicillin Sodium for Injection (1.6 million units / bottle) 1
[0025] Sterile normal saline 4.5 ml
[0026] Streptomycin Sulfate Injection Preparation:
[0027] Streptomycin Sulfate for Injection (1 million units / bottle) 1
[0028] Sterile normal saline 5 ml
[0029] Use a disposable sterile syringe to draw sterilized saline, inject it into penicillin sodium or streptomycin sulfate bottle, shake it gently, and store it at -20°C after use;
[0030] (2) To prepare phytohemagglutinin (PHA) solution, draw a certain amount of sterilized distilled water with a disposable sterile syringe to dissolve 10 tubes (10 mg per tube) of PHA powder.
[0031] (3) Culture medium preparation
[0032] First, ...
Embodiment 2
[0033] Example 2 Culture and Chromosome Preparation of Human Peripheral Blood Lymphocytes (Application in Culture of Human Peripheral Blood Lymphocytes and Chromosome Karyotype Analysis).
[0034] (1) Peripheral blood collection: Disinfect the skin at the cubital vein with iodophor alcohol, and after it is completely dry, collect 2 ml of blood with a 2.5 ml disposable sterile syringe, draw 0.2 ml of heparin sodium injection immediately, put the syringe flat, and mix gently .
[0035] (2) Inoculation culture: After the medium returns to room temperature, remove the aluminum sheet on the medium with tweezers, disinfect the rubber stopper with alcohol, and place it until the alcohol is completely evaporated. Discard the first few drops of blood from the syringe, operate under an alcohol lamp, insert the needle into the medium bottle obliquely through the rubber stopper at an angle of 45 degrees, inject 0.6 ml of whole blood drop by drop, mix gently and set the temperature at 37°C...
Embodiment 3
[0046] Example 3 Comparison of lymphocyte transformation rate and lymphocyte division index in chromosomal medium
[0047] Two brands of chromosome culture medium commonly used by Hebei Provincial Children's Hospital and the First Hospital of Hebei Medical University were selected, numbered ET and YD. According to Example 1, the chromosome culture medium of the present invention was prepared, different batches of medium labels A1-A6 were selected, and 0.6 ml of the blood of the same individual was respectively inoculated, and cell culture and chromosome preparation were carried out according to Example 2. The comparison of cell culture effects is shown in Table 1. It can be seen from Table 1 that the transformation rate and division index of the chromosome culture medium of the present invention are better than those of the other two brands, and the growth and proliferation effect of the cells is better. And from the comparison between different batches of A1-A6, the numerica...
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