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Green method for increasing enzymolysis efficiency of cellulose in rice straws through stepwise pretreatment with two deep co-melting solvents

A technology of deep eutectic solvent and rice straw, applied in fermentation and other directions, can solve the problems of poor pretreatment effect and low utilization efficiency, and achieve the effects of improving utilization rate, improving glucose yield, and enhancing enzymatic hydrolysis efficiency.

Inactive Publication Date: 2017-05-31
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The combination of two deep eutectic solvents and step-by-step pretreatment of lignocellulose overcomes the shortcomings of poor pretreatment effect and low utilization efficiency of some single deep eutectic solvents

Method used

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  • Green method for increasing enzymolysis efficiency of cellulose in rice straws through stepwise pretreatment with two deep co-melting solvents
  • Green method for increasing enzymolysis efficiency of cellulose in rice straws through stepwise pretreatment with two deep co-melting solvents
  • Green method for increasing enzymolysis efficiency of cellulose in rice straws through stepwise pretreatment with two deep co-melting solvents

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Using choline chloride / oxalic acid (molar ratio 2:1, deep eutectic solvent A) and choline chloride / urea (molar ratio 1:2, deep eutectic solvent B) in two steps to improve rice straw enzymes solution efficiency

[0023]a) Pretreatment: Accurately weigh 300mg of rice straw powder (the rice straw is dried and crushed into rice straw powder with an average particle size of 150-350μm) and 6g of choline chloride / oxalic acid at a molar ratio of 2:1. Deep eutectic solvent A, put together in a 25mL Erlenmeyer flask, reflux and stir at 120°C for 2 hours; then cool to room temperature, add 6g of warm water to dilute, filter, then wash the filter residue with 18g of deionized water for 4 times, and dry the filter residue , and then add 6g of choline chloride / urea with a molar ratio of 1:2 deep eutectic solvent B and put them together in a 25mL Erlenmeyer flask, reflux and stir at 120°C for 2 hours; then cool to room temperature, add 6g of warm water to dilute, After filtering, the...

Embodiment 2

[0028] Improve rice straw enzymes by pretreatment with choline chloride / urea (molar ratio 1:2, deep eutectic solvent B) and choline chloride / oxalic acid (molar ratio 2:1, deep eutectic solvent A) solution efficiency

[0029] a) Pretreatment: Accurately weigh 300 mg of rice straw powder (the rice straw is dried and crushed into rice straw powder with an average particle size of 150-350 μm) and 6 g of deep eutectic solvent B, and place them together in a 25 mL Erlenmeyer flask. Reflux and stir at 120°C for 2 hours; then cool to room temperature, add 6g of warm water to dilute and filter, then wash the filter residue with 18g of deionized water for 4 times, after the filter residue is dried, add 6g of deep eutectic solvent A and place it in a 25mL triangular In the bottle, reflux and stir at 120 ° C for 2 hours; then cool to room temperature, add 6 g of warm water to dilute, filter, and then wash the filter residue with 18 g of deionized water for 4 times, and dry the filter resi...

Embodiment 3

[0046] Malic acid / proline (molar ratio 1:3, deep eutectic solvent A) and choline chloride / urea (molar ratio 1:2, deep eutectic solvent B) were pretreated in two steps to improve rice straw enzymes solution efficiency

[0047] a) Pretreatment: Accurately weigh 300mg of rice straw powder (the rice straw is dried and crushed into rice straw powder with an average particle size of 150-350μm) and 6g of malic acid / proline with a molar ratio of 1:3 Eutectic solvent A, put together in a 25mL Erlenmeyer flask, reflux and stir at 120°C for 6 hours; then cool to room temperature, add 6g of warm water to dilute, filter, and then wash the filter residue with 18g of deionized water for 4 times, and then dry the filter residue Add 6g of choline chloride / urea deep eutectic solvent B with a molar ratio of 1:2, put them in a 25mL Erlenmeyer flask, reflux and stir at 120°C for 6 hours; then cool to room temperature, add 6g of warm water to dilute and filter, The filter residue was washed 4 time...

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Abstract

The invention belongs to the fields of utilization of lignocelluloses and energy and chemical industry application, and discloses a green method for increasing enzymolysis efficiency of cellulose in rice straws through stepwise pretreatment with two deep co-melting solvents. The method includes the steps of: with one deep co-melting substance as a solvent, pre-treating the rice straws, and performing filtration and separation to obtain a residue; drying the residue and pre-treating the residue with the other one deep co-melting solvent in the same way, thus finally obtaining pre-treated rice straws; enzymolyzing the rice straw residue, as a substrate, with cellulase to finally obtain a saccharide liquid mainly comprising glucose. The method increases the enzymolysis efficiency of the rice straws, increases yield of fermentable reduced saccharide (glucose), overcomes defects that an ionic liquid pretreatment process is high in cost and is not environment-friendly, and also solves a problem of poor pretreatment effect with single deep co-melting solvent. The method also expands application range and increases utilization rate of some green deep co-melting solvents.

Description

technical field [0001] The invention belongs to the fields of lignocellulose utilization and energy chemical industry application, and specifically relates to a green method for improving the enzymatic hydrolysis efficiency of cellulose in rice straw by using two deep eutectic solvents for step-by-step pretreatment. Background technique [0002] The production of biofuels and related platform compounds from a wide range of sources, cheap and renewable lignocellulosic biomass such as agricultural waste is one of the most potential paths to solve the current global environmental pollution and energy crisis. The main components of lignocellulosic biomass are cellulose, hemicellulose, and lignin. Due to differences in species, sources, and parts, the structures and proportions of these three types of biopolymers are also different. At present, the effective conversion of lignocellulosic biomass covers the biorefinery industry that fully utilizes biomass components, including obt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14C12P2201/00C12P2203/00
Inventor 侯雪丹黄超梅叶梅林凯鹏
Owner GUANGDONG UNIV OF TECH
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