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Electrochemical sensor for detecting isocarbophos and acetamiprid at same time and method system of electrochemical sensor

A technology of isocarbophos and acetamiprid, applied in the field of sensors, can solve the problems of complicated instrument operation and inability to achieve high throughput

Active Publication Date: 2017-06-13
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the shortcomings of complex instrument operation, professional operators and inability to achieve high throughput in the existing detection methods, and provides an electrochemical sensor and its method system for simultaneously detecting isocarbophos and acetamiprid. Electrochemical Detection of Isocarbophos and Acetamiprid by Ligand

Method used

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  • Electrochemical sensor for detecting isocarbophos and acetamiprid at same time and method system of electrochemical sensor
  • Electrochemical sensor for detecting isocarbophos and acetamiprid at same time and method system of electrochemical sensor
  • Electrochemical sensor for detecting isocarbophos and acetamiprid at same time and method system of electrochemical sensor

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Experimental program
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Effect test

Embodiment 1

[0072] a. The gold electrode is first polished in the alumina slurry with a particle size of 0.3 μm and the alumina slurry with a particle size of 0.05 μm until it becomes a mirror surface, and the electrode is soaked in 20 mL of H with a volume ratio of 3:1. 2 SO 4 and H 2 o 2 In the mixed solution, wash thoroughly with ultrapure water after incubation for 20 min;

[0073] b. Add a total of 6 μL of XB solution (10 μM XB1: 2 μL and 10 μM XB2: 4 μL) onto the electrode surface treated in step a, and incubate at room temperature for 2 hours. The chain is fixed to the electrode surface.

[0074] c. Use MCH modification to block the non-specific binding sites, specifically: add 6 μL of 1 mM MCH solution to the electrode surface dropwise, incubate for 30 min, and wash with PBS.

[0075] d, carry out homogeneous reaction, main steps are:

[0076] 1. Add 2 μL of 100 nM HAP1, 2 μL of 100 nM HAP2 and 20 μL of 1× Phi29 DNA polymerase buffer solution into the centrifuge tube, and inc...

Embodiment 2

[0084] a. The gold electrode is first polished in the alumina slurry with a particle size of 0.3 μm and the alumina slurry with a particle size of 0.05 μm until it becomes a mirror surface, and the electrode is soaked in 20 mL of H with a volume ratio of 3:1. 2 SO 4 and H 2 o 2 In the mixed solution, wash thoroughly with ultrapure water after incubation for 20 min;

[0085] b. Add a total of 6 μL of XB solution (10 μM XB1: 2 μL and 10 μM XB2: 4 μL) onto the electrode surface treated in step a, and incubate at room temperature for 2 hours. The chain is fixed to the electrode surface.

[0086] c. Modification of MCH to block unspecific binding sites.

[0087]d. The main steps in the homogeneous reaction:

[0088] 1. Add 2 μL of 100 nM HAP1, 2 μL of 100 nM HAP2 and 20 μL of 1× Phi29 DNA polymerase buffer solution into the centrifuge tube, add excess isocarbophos (500 ppb), and incubate at 37 ° C for 30 min;

[0089] 2. Continue to add 2 μL of 100 nM primer 1 and 2 μL of 100...

Embodiment 3

[0095] a. The gold electrode is first polished in the alumina slurry with a particle size of 0.3 μm and the alumina slurry with a particle size of 0.05 μm until it becomes a mirror surface, and the electrode is soaked in 20 mL of H with a volume ratio of 3:1. 2 SO 4 and H 2 o 2 In the mixed solution, wash thoroughly with ultrapure water after incubation for 20 min;

[0096] b. Add a total of 6 μL of XB solution (10 μM XB1: 2 μL and 10 μM XB2: 4 μL) onto the electrode surface treated in step a, and incubate at room temperature for 2 hours. The chain is fixed to the electrode surface.

[0097] c. Modification of MCH to block unspecific binding sites.

[0098] d. The main steps in the homogeneous reaction:

[0099] 1. Add 2 μL of 100 nM HAP1, 2 μL of 100 nM HAP2 and 20 μL of 1× Phi29 DNA polymerase buffer solution into the centrifuge tube, add excess acetamiprid (500 ppb), and incubate at 37 ° C for 30 min;

[0100] 2. Continue to add 2 μL of 100 nM primer 1 and 2 μL of 100...

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Abstract

The invention discloses an electrochemical sensor for detecting isocarbophos and acetamiprid at same time and a method system of the electrochemical sensor; the electrochemical sensor comprises an electrode, a beacon probe layer, a 6-mercaptohexan-1-ol (MCH) closure layer, and a homogenous reaction product layer sequentially from inside to outside; the beacon probe layer is modified with methylene blue and ferrocene having electrochemical signals not mutually affected; a preparation method comprises: polishing the electrode, treating with H2SO4 and H2O2, and modifying the surface of the electrode with a beacon probe. The electrochemical sensor according to the invention is simple to prepare and has good performance stability, the electrode is highly repeatable, and the electrochemical sensor is applicable to the detection of isocarbophos and acetamiprid in terms of food safety and the actual application of biosensor industrialization; the electrochemical sensor can provide quick online detection for isocarbophos and acetamiprid in food, and isocarbophos and acetamiprid detection limits are 0.12 ppb and 0.37 ppb respectively.

Description

technical field [0001] The invention relates to a method for detecting two pesticides in the technical field of sensors, in particular to an electrochemical sensor and a method system for simultaneously detecting isocarbophos and acetamiprid. Background technique [0002] Organophosphorus is widely used in insecticide, fungicide, and weed control of crops, and is the most widely used pesticide in my country. Organophosphorus pesticides are neurotoxic substances, which mainly inhibit the activity of acetylcholinesterase in blood and tissues, resulting in a large accumulation of acetylcholine, thereby blocking nerve conduction and causing central nervous system poisoning. Isocarbophos is a highly toxic organophosphorus insecticide that can cause poisoning through the esophagus, skin and respiratory tract. As a highly toxic pesticide, isocarbophos is prohibited from being used on fruits, tea, tobacco, vegetables, Chinese herbal medicines and other plants Or on vegetables and f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/38G01N27/26
CPCG01N27/26G01N27/30G01N27/38
Inventor 徐霞红郭玉娜王新全王祥云王强杨华齐沛沛汪志威
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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