Complex inoculant and application thereof in degrading cellulose in poultry and livestock manure

Abstract

The invention relates to a complex inoculant and an application thereof in degrading cellulose in poultry and livestock manure. The complex inoculant consists of a bacterium strain which is cultivated in a beef extract-peptone medium and a fungus strain which is cultivated in a PDA culture medium at the volume ratio of a culture solution of 1:1. According to the complex inoculant provided by the invention, optimum enzyme-production temperatures of the various strains correspond to temperatures in different stages of composting fermentation, and changing curves of the two parts (the optimum enzyme-production temperatures of the various trains and the temperatures in different stages of the composting fermentation) fit and are matched; the various strains grow in a coordinated mode and continuously produce enzyme, so that a relatively stable cellulase producing environment is formed, and stable enzyme activity is guaranteed; the enzyme can be continuously produced in different fermentation temperature stages of a composting process, so that decomposition of the cellulose in the poultry and livestock manure is promoted; and since a reaction substrate is continuously utilized by the various strains, a level and a peak of cellulase activity of composting fermentation of the poultry and livestock manure cellulase can be also improved in advance and the fermentation time of a compost can be shortened, so that adaptability and a decomposing effect of the compost are enhanced.

Description

technical field [0001] The invention relates to the field of poultry manure treatment, in particular to a composite microbial agent and its application in degrading cellulose in poultry manure. Background technique [0002] Our country produces as many as 3 billion tons of poultry manure every year. The cellulose content in poultry manure is high, which is an important part of renewable resources. Natural composting of poultry and livestock manure has a slow aging speed and is not easy to heat up. It does not meet the harmless treatment standards of compost in China. Adding fermentation bacteria to compost can promote the maturation process of compost and improve the quality of compost. The principle is Cellulase is produced by fermenting bacterial agents, and cellulase decomposes cellulose into glucose, transforming abundant cellulose waste resources into renewable resources, so that it can not only realize the harmless treatment of poultry and livestock manure, but also so...

AI Technical Summary

Problems solved by technology

[0003] However, the maturing process of poultry and livestock manure is a process of constant temperature changes. Since the microbial strains of the fermenting agent produce cellulase, there is a certain optimum temperature. When the compost temperature is lower or higher than the optimum temperature, the fermentation The enzyme production efficiency of the bacterial agent is low or even no enzyme production, resulting in a low efficiency of cellulose degradation in the process of poultry manure composting, which takes a long time

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