Bone peptide extracting solution and preparation method and application thereof in aspect of bone marrow injury caused by chemotherapy

A technology of extracting liquid and bone peptide, applied in the field of preparation and application of peptides, can solve the problem that new uses of bone marrow damage have not yet been explored, and achieve the effects of preventing and treating osteoporosis, inhibiting the rise of blood pressure, and preventing and treating osteoarthritis.

Inactive Publication Date: 2017-07-07
HUNAN TIANJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, pig bones are extracted by enzymatic hydrolysis, and the obtained bone peptide extract has functions such as antihypertension, prevention and treatment of osteoar

Method used

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  • Bone peptide extracting solution and preparation method and application thereof in aspect of bone marrow injury caused by chemotherapy
  • Bone peptide extracting solution and preparation method and application thereof in aspect of bone marrow injury caused by chemotherapy
  • Bone peptide extracting solution and preparation method and application thereof in aspect of bone marrow injury caused by chemotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of bone peptide extract, comprising the following steps:

[0025] (1) After washing 100g of pork bones, cook them at a temperature of 105°C for 2-6 hours until the bones become crisp;

[0026] (2) Crush the crispy bones until the particle size is less than 1 cm, add water according to the solid-to-liquid ratio of 1:1 and mix them, then use a rubber mill to grind the bones until the bone paste is rubbed by hand without any gritty feeling;

[0027] (3) Add hydrochloric acid to the bone paste to adjust the pH value to 2, add 0.2 g of pepsin, stir the acidified and enzymolyzed bone paste mixture at 37°C for 48 hours, and set aside;

[0028] (4) Mix the bone paste mixture with water and decoct twice, add 200g of water for the first decoction for 1.5 hours; crude filtrate;

[0029] (5) Adjust the crude filtrate to pH 7.5 with sodium hydroxide, keep the temperature at 37.5-45° C., add 0.2 g of trypsin for enzymolysis, and the material after enzymolysis i...

Embodiment 2

[0032] A preparation method of bone peptide extract, comprising the following steps:

[0033] (1) After washing 100g of pork bones, cook them at a temperature of 105°C for 2-6 hours until the bones become crisp;

[0034] (2) Crush the crispy bones until the particle size is less than 1 cm, add water according to the solid-to-liquid ratio of 1:1 and mix them, then use a rubber mill to grind the bones until the bone paste is rubbed by hand without any gritty feeling;

[0035] (3) Add hydrochloric acid to the bone paste to adjust the pH value to 4, add 0.2 g of pepsin, stir the acidified and enzymolyzed bone paste mixture at 35°C for 72 hours, and set aside;

[0036] (4) Mix the bone paste mixture with water and decoct twice, add 200g of water for the first decoction for 1.5 hours; crude filtrate;

[0037] (5) Adjust the crude filtrate to pH 6.5 with sodium hydroxide, keep the temperature at 37.5-45° C., add 0.2 g of trypsin for enzymolysis, and the material after enzymolysis i...

Embodiment 3

[0040] A preparation method of bone peptide extract, comprising the following steps:

[0041] (1) After washing 100g of pork bones, cook them at a temperature of 105°C for 2-6 hours until the bones become crisp;

[0042] (2) Crush the crispy bones until the particle size is less than 1 cm, add water according to the solid-to-liquid ratio of 1:1 and mix them, then use a rubber mill to grind the bones until the bone paste is rubbed by hand without any gritty feeling;

[0043] (3) Add hydrochloric acid to the bone paste to adjust the pH to 1, add 0.2 g of pepsin, stir the acidified and enzymatically hydrolyzed bone paste mixture at 37°C for 12 hours, and set aside;

[0044] (4) Mix the bone paste mixture with water and decoct twice, add 200g of water for the first decoction for 1.5 hours; crude filtrate;

[0045] (5) Adjust the crude filtrate to pH 7.5 with sodium hydroxide, keep the temperature at 37.5-45° C., add 0.2 g of trypsin for enzymolysis, and the material after enzymo...

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PUM

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Abstract

The invention discloses a bone peptide extracting solution and a preparation method and application thereof in treatment of bone marrow injury. A high-content bone peptide extract is obtained by mixing and extracting acid and alkali and enzymes to prevent gastric mucosa ulcer, suppress blood pressure rise, improve bone strength, promote skin collagen metabolism to delay aging, promote the absorption of minerals, and also to resist hypertension, prevent and treat osteoarthritis and osteoporosis, treat gastric ulcer and other diseases, and resist aging and oxidation. Most importantly, the peptide extract can effectively prevent and treat bone marrow suppression and other adverse effects easily occurring in the current cancer chemotherapy, effectively prevent and treat bone marrow injury peripheral blood and bone marrow hematopoietic function, and improve bone marrow hyperplasia.

Description

technical field [0001] The invention relates to the preparation and application of bone peptide, in particular to the preparation and application of a polypeptide for preventing and treating bone marrow damage caused by tumor chemotherapy. Background technique [0002] At present, cancer treatment methods mainly include surgery, radiotherapy and chemotherapy, and chemotherapy has become the choice of many patients with advanced tumors. However, most chemotherapeutic drugs play a role in killing cells by damaging nuclear DNA or interfering with DNA synthesis. All have killing effects, resulting in gastrointestinal reactions, bone marrow suppression, hair loss, fatigue, immunosuppression, or damage to the heart, kidneys, and nervous system. Bone marrow suppression is the most important adverse reaction. Clinically, pancytopenia occurs, followed by severe infection, hemorrhage, failure, and death. Bone marrow suppression is a common toxic reaction of most chemotherapy drugs....

Claims

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Application Information

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IPC IPC(8): A61K38/02A61K35/32A61P7/00A61P7/06
CPCA61K35/32A61K38/02
Inventor 许必祥
Owner HUNAN TIANJIN PHARMA
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