Hollow hydroxyapatite microspheres with controllable pore size, preparation method and application thereof

A technology of hydroxyapatite and microspheres is applied in chemical instruments and methods, medical preparations with non-active ingredients, pharmaceutical formulations, etc. Effects of Biocompatibility and Degradability

Active Publication Date: 2019-02-22
乔威 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pore size of the hollow hydroxyapatite prepared in the prior art is uncontrollable, resulting in the inability to flexibly adjust the drug loading

Method used

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  • Hollow hydroxyapatite microspheres with controllable pore size, preparation method and application thereof
  • Hollow hydroxyapatite microspheres with controllable pore size, preparation method and application thereof
  • Hollow hydroxyapatite microspheres with controllable pore size, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The preparation of step A, calcium carbonate precursor:

[0062] Take 250 mL of calcium chloride solution with a concentration of 0.2 mol / L and mix it with 250 mL of sodium lauryl sulfate solution with a concentration of 1 mg / mL, add 250 mL of 0.2 mol / L sodium carbonate solution to the mixed solution after half an hour, and stir for 1.5 hour, filter to obtain the calcium carbonate precipitate, filter and wash the calcium carbonate precipitate 8 times with ultrapure water, then filter and wash the calcium carbonate precipitate 2 times with absolute ethanol, and finally dry the washed calcium carbonate precipitate at 120°C for 24 hours;

[0063] Step B, hydrothermal reaction:

[0064] Get the calcium carbonate precursor 1g that obtains in step A and mix with 0.2mol / L disodium hydrogen phosphate solution, control the mol ratio of calcium element and phosphorus element to be 1.67, be the ammoniacal liquor of 33% with mass percentage concentration Adjust the pH value to 10....

Embodiment 2

[0068] The preparation of step A, calcium carbonate precursor:

[0069] Take 250 mL of calcium chloride solution with a concentration of 0.2 mol / L and 250 mL of sodium lauryl sulfate solution with a concentration of 2.33 mg / mL and mix them. After half an hour, add 250 mL of 0.2 mol / L sodium carbonate solution to the mixed solution, stir After 1.5 hours, filter to get the calcium carbonate precipitate, filter and wash the calcium carbonate precipitate with ultrapure water 8 times, then filter and wash the calcium carbonate precipitate with absolute ethanol for 2 times, and finally dry the washed calcium carbonate precipitate at 120°C for 24 hours;

[0070] Step B, hydrothermal reaction:

[0071] Get the calcium carbonate precursor 1g that obtains in step A and mix with 0.2mol / L disodium hydrogen phosphate solution, control the mol ratio of calcium element and phosphorus element to be 1.67, be the ammoniacal liquor of 33% with mass percentage concentration Adjust the pH value t...

Embodiment 3

[0075] The preparation of step A, calcium carbonate precursor:

[0076] Take 250 mL of calcium chloride solution with a concentration of 0.2 mol / L and mix it with 250 mL of sodium lauryl sulfate solution with a concentration of 4.66 mg / mL, add 250 mL of 0.2 mol / L sodium carbonate solution to the mixed solution after half an hour, and stir After 1.5 hours, filter to get the calcium carbonate precipitate, filter and wash the calcium carbonate precipitate with ultrapure water 8 times, then filter and wash the calcium carbonate precipitate with absolute ethanol for 2 times, and finally dry the washed calcium carbonate precipitate at 120°C for 24 hours;

[0077] Step B, hydrothermal reaction:

[0078] Get the calcium carbonate precursor 1g that obtains in step A and mix with 0.2mol / L disodium hydrogen phosphate solution, control the mol ratio of calcium element and phosphorus element to be 1.67, be the ammoniacal liquor of 33% with mass percentage concentration The pH value was ad...

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Abstract

The invention provides a hollow hydroxyapatite microsphere with a controllable bore diameter. The grain size of the microsphere is 400nm-7000nm; the microsphere surface has a thorn-shaped structure; a hollow structure is formed in the microsphere; and the crystal form is hydroxyapatite. The preparation method for the microsphere mainly comprises the following steps: preparing a calcium carbonate precursor, performing hydrothermal reaction and marking with rhodamine. The invention also researches an application of the microsphere in the field of a drug-loading slow release. After the invention researches the drug-loading efficiency, degradation rate and release rule of the microsphere prepared from lauryl sodium sulfate in different concentrations, a conclusion proves that the acquired hollow hydroxyapatite microsphere can achieve the most ideal drug-loading and drug-release effects when the concentration of the lauryl sodium sulfate is a critical micelle concentration. Besides, the invention innovatively realizes the combination of the microsphere with the rhodamine dye through an amino group on the surface of the hollow hydroxyapatite microsphere, so that the drug-loading microsphere is more easily positioned and traced in biological performance evaluation and action mechanism research.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a hollow hydroxyapatite microsphere with controllable aperture, a preparation method and an application thereof. Background technique [0002] Hydroxyapatite [Ca 10 (PO 4 ) 6 (OH) 2 HA for short] is the main inorganic component of hard tissues such as human bones and teeth. It has good biocompatibility, biological activity, no toxic side effects, and no immune response. It can promote the growth of new bone after implantation in the body. It has been widely used in human hard tissue replacement materials and repair materials. As a new type of inorganic carrier material, hollow hydroxyapatite has become the focus of research in the interdisciplinary fields of biology, pharmacy and material science. [0003] In the preparation process of hydroxyapatite, adding surfactant to prepare hollow calcium carbonate precursor, forming hollow hydroxyapatite throu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B25/32A61K47/04A61K47/20
CPCA61K9/0002A61K47/02A61K47/20C01B25/325C01P2002/70C01P2002/72C01P2002/82C01P2004/03C01P2004/34C01P2004/51C01P2004/61C01P2004/62C01P2006/16
Inventor 兰新苗乔威苏宇雄杨伟国陈卓凡
Owner 乔威
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