Dinotefuran hapten, complete antigen and antibody, as well as preparation method and application thereof

A technology of dinotefuran and hapten, which is applied in the field of dinotefuran hapten, complete antigen and antibody and preparation thereof, and achieves the effects of simple method, high yield and simple synthesis

Active Publication Date: 2017-07-07
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about dinotefuran artificial hapten, artificial antigen, antibody and immunoassay method based on it

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  • Dinotefuran hapten, complete antigen and antibody, as well as preparation method and application thereof
  • Dinotefuran hapten, complete antigen and antibody, as well as preparation method and application thereof
  • Dinotefuran hapten, complete antigen and antibody, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation method of dinotefuran hapten includes:

[0042] (1) Dissolve 2.10g of dinotefuran in 25mL ethanol in an anhydrous three-necked flask, connect the condenser, and heat to reflux the ethanol;

[0043] (2) Pour nitrogen protection into the three-necked flask, magnetically stir the above ethanol solution, slowly add 8.5g of stannous chloride dihydrate until it is completely dissolved, and react under nitrogen protection for 4 hours;

[0044] (3) After the reaction is completed, add distilled water to the three-necked flask and stir while adding until no turbidity is formed. Centrifuge the mixed solution at 12000 rpm / min for 10 minutes, discard the precipitate, and repeat the centrifugation 3 times;

[0045] (4) The supernatant was extracted 4 times with ethyl acetate, the extracts were combined, and vacuum rotary evaporated to dryness to obtain the target product.

[0046] The preparation method of the immunogen is the active ester method, which includes the following ...

Embodiment 2

[0058] The preparation method of dinotefuran hapten includes:

[0059] (1) Dissolve 2.40 g of dinotefuran in 25 mL of ethanol in an anhydrous three-necked flask, connect the condenser, and heat to reflux the ethanol;

[0060] (2) Pour nitrogen protection into the three-necked flask, magnetically stir the above ethanol solution, slowly add 10.00 g of stannous chloride dihydrate until it is completely dissolved, and react under nitrogen protection for 5 hours;

[0061] (3) After the reaction is completed, add distilled water to the three-necked flask and stir while adding until no turbidity is formed. Centrifuge the mixed solution at 12000 rpm / min for 10 minutes, discard the precipitate, and repeat the centrifugation 4 times;

[0062] (4) The supernatant was extracted 5 times with ethyl acetate, the extracts were combined, and vacuum-rotated to dryness to obtain the target product.

[0063] The preparation method of the immunogen is the active ester method, which includes the following st...

Embodiment 3

[0083] The preparation method of dinotefuran hapten, the steps include:

[0084] (1) Dissolve 2.10g of dinotefuran in 30mL ethanol in an anhydrous three-necked flask, connect the condenser, and heat to reflux the ethanol;

[0085] (2) Pour nitrogen protection into the three-necked flask, magnetically stir and stir the above ethanol solution, slowly add 8.5g of stannous chloride dihydrate until it is completely dissolved, and react under nitrogen protection for 6 hours;

[0086] (3) After the reaction is completed, add distilled water to the three-necked flask and stir while adding until no turbidity is formed. Centrifuge the mixed solution at 12000 rpm / min for 10 minutes, discard the precipitate, and repeat the centrifugation 3 times;

[0087] (4) The supernatant was extracted 4 times with ethyl acetate, the extracts were combined, and vacuum rotary evaporated to dryness to obtain the target product.

[0088] The preparation method of the immunogen is the active ester method, which incl...

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Abstract

The invention discloses a preparation method and application of a dinotefuran hapten, a complete antigen and an antibody. The preparation method comprises preparation of a neonicotinoids dinotefuran hapten with a tetrahydro-3-furan structure, the complete antigen and the antibody and establishing of a corresponding detection method. The detection method solves the problems that a common spectrophotometric method is low in sensitivity and cannot meet the requirements, and an immunoassay method which is high in sensitivity, low in cost and quick and easy to master is established. According to the preparation method, 1-methyl-2-amino-3-(tetrahydro-3-furan methyl) guanidine is used as a hapten structure, coupled bovine serum albumin is used as an immunogen, and coupled egg albumin is used as a coating antigen; the immunogen passes through an immune animal, and the antibody is extracted from antiserum; a dinotefuran immunoassay method established on the basis of the antibody has the characteristics of high sensitivity and high specificity; furthermore, a hapten synthesis method is simple and convenient, and has a relatively good application prospect.

Description

Technical field [0001] The invention belongs to the technical field of immunochemistry and residual biological analysis of small-molecule food environmental pollutants, and specifically relates to a dinotefuran hapten, a complete antigen and an antibody, and a preparation method and application thereof. Background technique [0002] Dinotefuran is a new generation of nicotine pesticides developed by Mitsui Chemicals Co., Ltd., and is another class of nicotine pesticides with a new structure after the first generation of imidacloprid and the second generation of thiamethoxam. . The furan ring and the ring-opening guanidine group in the dinotefuran molecule replace the first two types of chloropyridyl, chlorothiazolyl or closed-ring guanidine group. It is the only nicotinic pesticide molecular structure that does not contain halogen atoms and aromatic rings The insecticidal activity and safety are higher. Dinotefuran is also a nicotinic acetylcholine receptor (nAChR) agonist. It ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/14C07K14/765C07K14/77C07K16/44C07K16/06C07K1/107G01N33/543
CPCC07D307/14C07K14/765C07K14/77C07K16/44C07K2317/10G01N33/543
Inventor 刘景坤崔艳梅李勤奋黄华平李叶邹雨坤
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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