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Method of accelerating in-situ degradation of PCP in paddy field

A paddy field, in-situ technology, applied in the field of remediation of polluted soil, can solve the problems of long degradation cycle, secondary pollution and high cost, and achieve the effects of fast degradation rate, easy operation and low cost of raw materials

Inactive Publication Date: 2017-07-14
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Physicochemical methods include adsorption (Chinese patent 201310031463.X), photocatalytic oxidation (Chinese patent 201110020264.X), electrochemical degradation (Chinese patent 201410103417.0), these degradation methods are costly and prone to secondary pollution
The biodegradation method of PCP in paddy fields (ChenY.T., Environmental Science and PollutionResearch, 23(2016), 23184-23194) has very low efficiency and long degradation cycle
Although external application of lactate in rice fields can improve the degradation efficiency of PCP, its efficiency is still low (ChenM.J., Geoderma, 217(2014), 201-211)

Method used

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  • Method of accelerating in-situ degradation of PCP in paddy field
  • Method of accelerating in-situ degradation of PCP in paddy field

Examples

Experimental program
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Effect test

Embodiment example 1

[0013] Implementation Case 1: The promotion test of sodium citrate on the degradation of PCP in the soil solution system.

[0014] Paddy soil contaminated by PCP was collected from Guangdong Province, air-dried and sieved. 0.5g (dry weight) of soil, 10ml of PIPES buffer solution, and PCP with a final concentration of 5mg / L were added to a 20ml sterilized vial. Add 5mmol / L, 10mmol / L, 12mmol / L, 15mmol / L of sodium citrate respectively, culture in anaerobic and dark-proof biochemical incubator at 30°C, and take regular samples for analysis. Mainly detect PCP concentration, metabolite concentration, Fe(II) concentration and extract DNA to analyze the composition of microbial community, use ultrasonic vibration to extract residual PCP in the solution, high performance liquid chromatography to detect the residual concentration of PCP, and GC-MS to detect the metabolites of PCP , UV-Vis spectrophotometer to detect Fe (Ⅱ) concentration, and extract DNA application pyrosequencing. The...

Embodiment example 2

[0017] Implementation case 2: PCP in situ degradation test in paddy field.

[0018] In this case, the on-site repair test was adopted. The experimental site is a paddy field polluted by PCP in Dongpu Town, Zhongshan City, Guangdong Province, and the concentration of PCP is about 40ng / g (dry weight). First, the paddy field was plowed and leveled to keep it flooded, and then it was divided into 12 square paddy field grids of 2m×2m in total, with a 100cm gap between each grid. The mass of sodium citrate applied in each grid was 0g, 15g, 30g and 40g respectively; each application amount was repeated three times, except that the application amount of sodium citrate was different, all other conditions were the same. Then rice was planted according to the normal rice planting procedure, fertilization, weeding, keeping the flooded state, and keeping the water level of all treatments consistent, and continuously measuring the concentration change of PCP. The results are shown in Tabl...

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Abstract

The invention belongs to the technical field of biology, and relates to a repair method of contaminated soil, in particular to a method of accelerating in-situ degradation of PCP (pentachlorophenol) in a paddy field. The method of accelerating the in-situ degradation of PCP (pentachlorophenol) in the paddy field is characterized in that electron donor sodium citrate is used in an anaerobic environment to promote iron-reducing bacteria in soil of the paddy field to reduce iron oxides to Fe(II), and produced Fe(II) can promote PCP dechlorinating transformation till PCP is mineralized finally. With the adoption of the method, PCP in the paddy field can be degraded effectively, the problems of poisoning and various human diseases that are caused by the fact that PCP enters a food chain can be solved fundamentally. The method has the advantages that the damage to the environment is very little, no secondary pollution is produced, the cost is low, large-scale operation is facilitated, and no production or economic income is affected.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a method for repairing polluted soil. It is suitable for rapid treatment of PCP pollution under flooded anaerobic conditions such as paddy fields, water bodies, wetlands, etc., and can make the concentration of PCP pollution after treatment reach the national standard. Background technique [0002] Pentachlorophenol was once widely used in the Yangtze River Basin in my country as the main pesticide for the control of snails, the host of schistosomiasis, with 1.48×10 8 m 2 It has been used continuously for decades in rice fields and ponds. Under normal conditions, PCP is not easy to be oxidized, difficult to hydrolyze, has low volatility, and is difficult to migrate through the air, resulting in serious pesticide pollution to rice fields, wetlands, and water bodies. These pesticides will be absorbed by plants such as rice and accumulate through the food chain, eventually producing str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/08
CPCB09C1/08B09C2101/00
Inventor 牟山陶亮唐先忠
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
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