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Preparation method of high-concentration acid-soluble collagen solution and water-soluble collagen solution

An acid-soluble, high-concentration technology, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of small processing capacity, long preparation cycle, long cycle, etc., and achieve the effect of reducing viscosity and improving solubility

Active Publication Date: 2020-02-28
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cycle of natural evaporation and concentration is too long, and it usually takes several weeks to reach the target concentration, and the processing capacity of this method is too small to achieve mass production
Evaporating the solvent under negative pressure can shorten the preparation cycle slightly, but the preparation cycle is still too long
And the evaporation concentration method leads to uneven concentration of the inner and outer layers of the collagen solution, and in extreme cases even causes the surface layer of collagen to dry and form a film

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Cut the cowhide into small pieces and grind them in a meat grinder. Put the minced cowhide with a wet weight of 10g into an Erlenmeyer flask, add 0.3g of pepsin and 500mL of acetic acid with a concentration of 0.5mol / L, then put the Erlenmeyer flask into a constant temperature shaker at 4°C to vibrate to extract collagen, shake The bed speed is set at 180r / min, and the working time is 72 hours. After shaking the extracted solution, take the supernatant after centrifugation, add sodium chloride to a concentration of 0.7mol / L for salting out, then centrifuge again, discard the supernatant, and dissolve the obtained crude collagen in 0.1mol / L acetic acid Dissolve, put the dissolved collagen solution into a dialysis bag, dialyze with deionized water for 72 hours, and finally vacuum freeze-dry for 72 hours to obtain an acid-soluble collagen sponge.

[0033] (2) Take 0.01mol of acetic acid and 30g of urea, prepare 1L of acetic acid-urea solution (the molar concentration ...

Embodiment 2

[0037] (1) Cut the pork skin into small pieces and put them into a meat grinder to grind. Put the minced pigskin with a wet weight of 10g into a 1L Erlenmeyer flask, add 0.3g of pepsin and 500mL of acetic acid with a concentration of 0.5mol / L, and then put the Erlenmeyer flask into a 4°C constant temperature shaker to shake, The working speed is 180r / min, and the working time is 72 hours. After the shaken solution is centrifuged, take the supernatant, add sodium chloride to a concentration of 0.7mol / L for salting out, then centrifuge again, discard the supernatant, dissolve the obtained crude collagen in 0.1mol / L acetic acid, and The dissolved collagen solution was put into a dialysis bag and dialyzed against deionized water for 72 hours, and finally vacuum freeze-dried for 72 hours to obtain an acid-soluble collagen sponge.

[0038](2) Take 0.05 mol of acetic acid and 5 g of thiourea, prepare 1 L of acetic acid-thiourea solution (the molar concentration of acetic acid is 0.5...

Embodiment 3

[0042] (1) Cut the fish skin into small pieces and grind them in a meat grinder. Put the minced fish skin with a wet weight of 10g into an Erlenmeyer flask, add 0.3g of pepsin and 500mL of acetic acid with a concentration of 0.5mol / L, and then put the Erlenmeyer flask into a constant temperature shaker at 4°C to vibrate. It is 180r / min, and the working time is 72 hours. After the shaken solution is centrifuged, take the supernatant, add sodium chloride to a concentration of 0.7mol / L for salting out, then centrifuge again, discard the supernatant, dissolve the obtained crude collagen in 0.1mol / L acetic acid, and The dissolved collagen solution was put into a dialysis bag and dialyzed against deionized water for 72 hours, and finally vacuum freeze-dried for 72 hours to obtain an acid-soluble collagen sponge.

[0043] (2) Take 0.25mol of acetic acid and 17.5g of urea to prepare 1L of acetic acid-urea solution (molar concentration of acetic acid is 0.25mol / L, concentration of ure...

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Abstract

The invention relates to a preparation method for high-concentration acid-soluble and water-soluble collagen solutions. The preparation method comprises the following steps: shearing collagen sponge into strips, carrying out dissolving in a solution containing a hydrogen bond breaker so as to achieve a required concentration, and carrying out dialysis and replacing the hydrogen bond breaker so as to obtain a high-concentration collagen solution. With the above-mentioned method provided by the invention, the collagen sponge is dissolved in a solvent containing the hydrogen bond breaker which can break a hydrogen bond formed in collagen, so the collagen in the solution is avoided from forming a collagen polymer; the viscosity of the collagen solution is reduced; the solubility of the collagen in the solution is improved; the concentration of the collagen in the solution is increased to 30 g / L or above; and through dialysis replacement of the solvent containing the hydrogen bond breaker, a high-concentration and uniform collagen solution is obtained. According to a scheme of the invention, the collagen sponge can be used for large-scale preparation of an acid-soluble collagen solution and a water-soluble collagen solution with uniform and high concentration in a short time.

Description

technical field [0001] The invention relates to the field of preparation of collagen solutions, in particular to methods for preparing high-concentration acid-soluble collagen solutions and water-soluble collagen solutions. Background technique [0002] Collagen is the main structural protein of the extracellular matrix and the protein with the highest content in organisms. It widely exists in human body and animal body. As a natural polymer material with a wide range of sources, collagen has many excellent properties, such as high affinity with cells, hemagglutination with platelets, promotion of wound convergence and crawling coverage of epidermal cells, and biodegradability. Widely used in the field of medicine and health. Collagen is usually extracted from animal skin in the form of a solution, and then freeze-dried to obtain a refined collagen sponge. This collagen is soluble in an acidic solution and is called acid-soluble collagen. Chinese patent application "A wate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/30C07K1/14
CPCC07K14/78C12P21/06
Inventor 张敏黄六莲陈礼辉杨俊晖吴慧苗庆显
Owner FUJIAN AGRI & FORESTRY UNIV
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