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Quality testing method for phenolic extract of dragon's blood and its preparation

A quality detection method, the technology of dragon's blood jerky phenol, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficulty in comprehensively measuring the quality of dragon's blood and the small number of tests, so as to ensure clinical effectiveness and rapid method , good reproducibility

Active Publication Date: 2019-10-29
BEIJING UNIV OF CHINESE MEDICINE
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing studies have shown that high isoflavanone compounds have the effects of inhibiting estrogen production, cytotoxicity, and antibacterial effects, and flavonoid oligomers have antibacterial, cytotoxic, and anti-inflammatory effects. However, the existing medicinal materials of dragon's blood The quality detection methods of the two types of components are not involved; in addition, for other types of phenolic compounds, the number of detections is also small (no more than 5), so it is difficult to fully measure the quality of dragon's blood

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  • Quality testing method for phenolic extract of dragon's blood and its preparation
  • Quality testing method for phenolic extract of dragon's blood and its preparation
  • Quality testing method for phenolic extract of dragon's blood and its preparation

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[0060]

[0061] The preparation of the test solution of the present invention is to dissolve the phenolic extract of dragon's blood with a solvent, and then filter through a microporous membrane to obtain the test solution. The solvent may be selected from one or more of methanol, ethanol, acetonitrile, aqueous methanol, aqueous ethanol or aqueous acetonitrile. Preferably, the volume percentage of methanol in the methanol aqueous solution is 30-95%; the volume percentage of ethanol in the ethanol aqueous solution is 30-95%, and the volume percentage of acetonitrile in the acetonitrile aqueous solution is 30-95%. Preferably, the solvent is methanol. The concentration of the test solution may be 0.3-3.0 mg / ml, preferably 0.5-2.0 mg / ml, more preferably 0.8-1.5 mg / ml. According to a preferred embodiment of the present invention, the concentration of the test solution is 1.0 mg / ml. The concentration of the test solution refers to the ratio of the weight of the phenolic extract ...

Embodiment 1

[0095] The quality detection method of embodiment 1-Longxue Tongluo Capsules

[0096] Preparation of reference substance solution: Accurately weigh the appropriate amount of 12 kinds of reference substances, add methanol to dissolve, so that the concentration of each component in the mixed reference substance solution is: 20.86 μg of 7,4′-dihydroxy-5-methoxyflavone / mL, 7,4′-dihydroxyflavone 39.69μg / mL, 7,4′-dihydroxy-5-methoxyhomoisoflavanone 55.60μg / mL, 7,4′-dihydroxyhomoisoflavanone Ketone 12.50μg / mL, Dragon Blood C 37.65μg / mL, 4,4′-dihydroxy-2-methoxydihydrochalcone 48.85μg / mL, 5,7,4′-Trihydroxy isoflavone Alkanone 34.32μg / mL, 3,4′-dihydroxy-5-methoxystilbene 35.43μg / mL, Socotra 39.31μg / mL, Dragon A 51.09μg / mL, Dragon Hemoglobin B 73.66 μg / mL and pterostylbene 79.51 μg / mL were obtained as reference substance solution.

[0097] Preparation of the test solution: Weigh an appropriate amount of Longxuejie Tongluo Capsules, dissolve them in methanol to a concentration of 1 ...

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Abstract

The invention discloses a quality detection method of a preparation of a phenol extract of dragon's blood. The method comprises the following steps: (1) preparing a test solution; (2) preparing a reference solution; and (3) adopting high performance liquid chromatography (HPLC) to measure the test solution and the reference solution; wherein octadecyl silane chemically bonded silica is taken as the filling agent of the HPLC column, the mobile phase is composed of acetonitrile A and a formic acid water solution B (0.1%), and gradient elution is adopted. The invention also discloses a quality detection method of a dragon's blood collateral dredging preparation, whose effective component is the phenol extract of dragon's blood. The method is rapid and accurate, and the repeatability is good.

Description

technical field [0001] The invention relates to a quality detection method of the phenolic extract of dragon's blood, and also relates to a quality detection method of a preparation of the phenolic extract of dragon's blood. Background technique [0002] Dracaena is the resin of Dracaena cochinchinenis (Lour) S.C.Chen, a plant of the genus Dracaena in the Liliaceae family. It is mainly distributed in Guangxi and Yunnan provinces in my country. Efficacy, clinically used to treat traumatic bleeding, blood stasis amenorrhea, sores, angina pectoris, myocardial infarction and other diseases. The effective part of the Chinese herbal medicine dragon’s blood——the phenolic extract of dragon’s blood, which is clinically used to treat the blood stasis syndrome in the meridians of stroke (mild to moderate cerebral infarction) in the recovery period, with remarkable curative effect. It is a traditional Chinese medicine with its active ingredient The second class of new drug - Longxue Ton...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/042
Inventor 李军屠鹏飞孙晶赵云芳郑姣张倩萧伟黄文哲
Owner BEIJING UNIV OF CHINESE MEDICINE