Application of manganese benzoate porphyrin in preparation of medicine for promoting diabetic corneal injury healing

A technology for manganese benzoic acid and corneal damage, applied in the field of biomedicine, can solve the problems of poor curative effect, slow effect, long treatment period, etc., and achieve the effects of repairing corneal damage, improving GSH level, and promoting corneal epithelial damage repair.

Inactive Publication Date: 2017-07-18
SHANDONG EYE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, although autologous serum, local application of insulin, naltrexone (related ligand of opioid growth factor receptor), gene therapy and other treatment methods can repair diabetic corneal damage, the above methods have long treatment cycle, slow effect and low curative effect. Poor, does not meet people's needs

Method used

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  • Application of manganese benzoate porphyrin in preparation of medicine for promoting diabetic corneal injury healing
  • Application of manganese benzoate porphyrin in preparation of medicine for promoting diabetic corneal injury healing
  • Application of manganese benzoate porphyrin in preparation of medicine for promoting diabetic corneal injury healing

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] TKE2 cells were conventionally cultured with KSFM medium (purchased from Invitrogen, USA), and 1×10 5 Inoculate cells into a six-well plate, select one well as a control, add 1mol / L mannitol and 30μl / ml cell solution to one well as the osmotic pressure control group, and add 1mol / L glucose and 30μl / ml cell solution to the other two wells , and at the same time add 50mmol / L MnTBAP 2μl / ml cell solution in one group of glucose treatment wells, so that the final concentration of MnTBAP is 100μM, and then in 5% CO 2 and cultured at 37°C. After 3 days, 50,000 cells were collected and incubated at 37°C for 20 min with a culture medium containing 5 μM fluorescent probe DCHF-DA (purchased from Molecular Probes, USA), and then used a microplate reader (model SpectraMaxM2, MolecularDevices, USA) measure the fluorescence intensity, that is, the ROS level.

[0033] A batch of cells treated in the same way was detected by JC-1 kit (purchased from Beyond, China) combined with flow cy...

Embodiment 2

[0037] Corneal epithelium damage repair and 8-OHdG expression in diabetic mice induced by streptozotocin (STZ, purchased from Sigma, USA).

[0038] Six STZ-induced adult C57BL / 6 diabetic mice and age-matched control mice (C57BL / 6 normal mice) were used to scrape the epithelium in the central 3mm area of ​​the right cornea of ​​the mouse with a 3mm trephine drill and an epithelial scraper, respectively. After 24, 48 and 72 hours, 0.25% sodium fluorescein was added dropwise to the cornea, and the cornea was observed and photographed under the slit lamp.

[0039] The result is as image 3 As shown, the staining of sodium fluorescein in the epithelial defect area is green, and the corneal epithelium of normal mice has healed 72 hours after injury, while the corneal epithelium of diabetic mice has not healed, which shows that in normal C57 mice and STZ-induced diabetic mice , after establishing the corneal epithelial scraping injury model, the corneal epithelial healing speed of d...

Embodiment 3

[0043] Effects of MnTBAP on the repair of STZ-induced corneal epithelial injury in diabetic mice

[0044] Twelve STZ-induced C57BL / 6 diabetic mice were randomly divided into a control group (diabetic mice) and a treatment group (diabetic mice + manganese benzoate porphyrin), and the central cornea of ​​the right eye of the mice was scraped using a 3 mm trephine drill and an epithelial spatula. Epithelium in the 3mm region, 7μl of 50mmol / L MnTBAP injected subconjunctivally in the right eye of the mice in the treatment group (mouse body weight is 25g), and 7μl PBS injected subconjunctivally in the right eye of the mice in the control group. After 72 hours, staining with sodium fluorescein and taking pictures under a slit lamp were used to observe the repair of epithelial damage in mice in each group.

[0045] The repair of epithelial injury in each group of mice was as follows: Figure 5 As shown, the fluorescein sodium staining area is the epithelial defect area, and it can be...

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Abstract

The invention provides application of manganese benzoate porphyrin in a preparation of a medicine for promoting diabetic corneal injury healing. In the application provided by the invention, the manganese benzoate porphyrin can release the mitochondrial DNA from the injury of the oxidative injury by playing the antioxidant free radical function, thereby playing the effect of healing diabetic corneal injury. The experiment proves that the manganese benzoate porphyrin (MnTBAP) can effectively inhibit the hyper-glucose group TKE2 cell ROS level, and can effectively improve the hyper-glucose induced TKE2 cell mitochondrial function dysfunction. In the STZ induced diabetic mice, a certain dosage of MnTBAP is injected under the conjunctiva after establishing a corneal epithelium curettage injury model, thereby effectively inhibiting the concentration of mice corneal epithelium ROS and inhibiting the 8-OHdG expression; and meanwhile, the GSH level of the corneal epithelium antioxidant and free radical scavenger of the diabetic mice can be improved, and the corneal epithelium injury healing of the diabetic mice is promoted.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of porphyrin manganese benzoate in the preparation of medicines for promoting the repair of diabetic corneal damage. Background technique [0002] With the increasing prevalence of diabetes worldwide, its ocular complications have become one of the main causes of blindness. At the same time, diabetic patients are very prone to lesions in various parts of the eye, including corneal lesions. Studies have shown that 47%-64% of diabetic patients may develop primary keratopathy, and its clinical features mainly include decreased corneal sensitivity, delayed epithelial healing, neurotrophic corneal ulcer and other symptoms. Diabetic patients may experience delayed corneal epithelial regeneration and even repeated peeling after cataract or retinal surgery. In addition, delayed repair of corneal damage may lead to various vision-threatening complications ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/409A61K47/02A61P27/02
CPCA61K31/409A61K9/0019A61K47/02
Inventor 周庆军曲明俐段豪云狄国虎董沐晨
Owner SHANDONG EYE INST
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