Preparation method of biocontrol microbial agent for controlling apple tree fungal diseases

A biocontrol agent, fungal technology, applied in the field of biocontrol agent preparation

Active Publication Date: 2017-07-21
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The problem to be solved by the present invention is to overcome the defects of the above-mentioned method of using chemical agents to prevent and control apple tree leaf diseases, and to provide a biocontrol method capable of preventing and controlling apple tree leaf fungal diseases such as apple anthracnose leaf blight and apple spot defoliation, as well as other diseases of apple trees. Bacteria - S

Method used

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  • Preparation method of biocontrol microbial agent for controlling apple tree fungal diseases
  • Preparation method of biocontrol microbial agent for controlling apple tree fungal diseases
  • Preparation method of biocontrol microbial agent for controlling apple tree fungal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Isolation, purification and screening of Streptomyces cacaoi FN

[0073] 1. Isolation and purification of Streptomyces cacaoi FN

[0074] Collect soil samples from Funing Vegetable Garden and put them into sterile bags, take 10g and put them into a sterilized triangular flask containing 100mL distilled water and glass beads, place on a shaker at 200rpm for 30min, let stand for 10min, shake well and use a pipette Draw 5mL from the liquid device and add it to a small triangle bottle containing 45mL sterile water, that is, dilute 10 2 times, sequentially serially diluted to 10 -3 、10 -4 、10 -5 、10 -6 Use a pipette to draw 100 μl and spread it on a 2216E plate containing antibiotics (potassium dichromate 50 mg / L, nalidixic acid 15 mg / L and nystatin 50 mg / L), and set 3 replicates for each concentration , placed in a 28°C incubator for 5-10 days. Use a sterilized bamboo stick to pick a single colony of actinomycetes and put it on a freshly prepared 2216E plate...

Embodiment 2

[0082] The growth characteristic of embodiment 2 cocoa Streptomyces FN

[0083] 1. Growth temperature

[0084] Dispense 2216E liquid culture medium (bacteria peptone No.2 5g / L, yeast powder 1g / L, the balance is distilled water, natural pH) into transparent test tubes, and sterilize with damp heat at 121°C for 30 minutes. There should be no precipitation in the culture medium. Streptomyces cocoa FN was inoculated with shaker flask 48 in 2216E liquid medium as an inoculant, and was inoculated into test tubes containing 2216E liquid medium at a rate of 1‰, and the control was not inoculated. Cultivate at 0°C, 7°C, 15°C, 20°C, 28°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C incubators for 3d-5d, set 3 repetitions, observe And record the growth (precipitation) situation.

[0085] 2. Acid and alkali resistance

[0086] Adjust the 2216E liquid medium with dilute HCl or NaOH to different pH (1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5 , 10.0, ...

Embodiment 3

[0101] Example 3 Application of Streptomyces cocoa FN to the prevention and treatment of fungal diseases of fruits and vegetables

[0102] Determination of the effect of Streptomyces cocoa FN on apple anthracnose leaf blight, apple leaf spot, apple ring spot, apple rot, apple root rot, apple blight rot, cucumber blight, cucumber blight, tomato early blight, Inhibition rate of watermelon wilt, rape sclerotinia and cabbage black spot pathogens. Draw a straight line through the center of the plate on the back of the PDA plate with a diameter of 90 mm, inoculate 7 mm of probiotic bacteria cake at two points 25 mm away from the center on the line, and inoculate 7 mm of blank PDA medium on the control plate. Inoculate the center of the PDA plate with a 7mm bacterial cake of pathogenic bacteria, and culture it at 28°C for 5-10 days. The radius of the control pathogenic bacteria and the radius of the treated pathogenic bacteria were counted respectively, and the antibacterial rate was...

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Abstract

The invention discloses a preparation method of a Streptomyces cacaoi biocontrol microbial agent for controlling apple tree fungal diseases and relates to the technical field of biological microbial agents. The preservation number of Streptomyces cacaoi is CGMCC No. 13080. The Streptomyces cacaoi biocontrol microbial agent has the advantages that the biocontrol microbial agent has an evident control effect on apple anthracnose and leaf blight and apple alternaria leaf spot and has a control effect on common apple tree leaf diseases and other apple tree diseases; the biocontrol microbial agent can completely replace a chemical control method and eliminate food potential safety hazard and is outstanding in control effectiveness on the common apple leaf diseases and outstanding in bacterium inhibiting spectrum broadness; in addition, the biocontrol microbial agent is high in fermentation activity, high in heat resistance, broad in bacterium inhibiting spectrum, capable of controlling common diseases such as cucumber gummy stem blight, cucumber seedling blight and tomato early blight of other fruits and vegetables, and the like.

Description

technical field [0001] The invention relates to the technical field of biological bacteria agents, in particular to a preparation method of a bio-control bacteria agent for preventing and treating fungal diseases of apple trees. Background technique [0002] During the growth of apple trees, common fungal diseases of apple trees mainly include apple anthracnose leaf blight, apple leaf spot, apple rot, and apple blight. Among them, apple anthracnose leaf blight (Glomerella leaf spot) is a new disease that has become popular in recent years. Professor Cao Keqiang and his research team from the Pest Control Research Office found that since 2009, every July in my country, fruit growers have responded one after another. Suspected occurrence of apple anthracnose leaf blight and serious damage. The disease is mainly found on varieties such as Gala, Golden Delicious, Qinguan and Jonagold. The research group of Professor Li Baohua of Qingdao Agricultural University isolated, culture...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/20C12N1/205C12R2001/465
Inventor 周天惠王灵敏刘辉孙振娜李玲娣
Owner 陕西枫丹百丽生物科技有限公司
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