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Extraction method of natural high-purity tea saponin

An extraction method and technology for tea saponin, which are applied in the field of extraction of natural high-purity tea saponin, can solve the problems that the extraction rate and purity of tea saponin cannot be directly used for makeup, environmental pollution, etc., so as to save extraction time and improve extraction efficiency. The effect of efficiency

Inactive Publication Date: 2017-07-25
林燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the commonly used extraction methods for tea saponin in Camellia oleifera seeds are water extraction, organic solvent extraction, acoustic wave-assisted extraction and microwave-assisted extraction. These methods not only use a large amount of organic solvents, but also easily cause environmental pollution. The extraction rate and purity of tea saponin cannot be directly used in cosmetics, so it is of great significance to develop a natural high-purity tea saponin extraction method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting natural high-purity tea saponin, comprising the following steps: (1) drying the tea dregs in a drying oven at 45°C for 12 hours, pulverizing them with a pulverizer, passing through a 60-mesh sieve, and using 4 times the amount of ethyl acetate The ester was heated and refluxed at 65°C for 2 hours, degreased, filtered and dried; (2) After mixing choline chloride and urea at a molar ratio of 1:2, heated at 500rpm and 80°C for 1.5 hours, a clear and uniform (3) After mixing the deep eutectic reagent and water uniformly at a volume ratio of 1:0.5, mix the degreased tea meal with the deep eutectic reagent-water mixture at a mass volume ratio of 1:8 Mixing, microwave-ultrasonic for 20 min under the conditions of 350W ultrasonic power, 200W microwave power and 40°C, and then filtered, the filter residue was extracted 3 times and the filtrate was combined. (4) Add 1 times methanol to the filtrate to precipitate the eutectic reagent, and dissolve tea sapo...

Embodiment 2

[0025] A method for extracting natural high-purity tea saponin, comprising the following steps: (1) drying the tea dregs in a drying oven at 50°C for 10 hours, pulverizing them with a pulverizer, passing through a 60-mesh sieve, and using 3 times the amount of petroleum ether Heating and reflux at 55°C for 2 hours, degreasing, filtering, and drying; (2) After mixing betaine and glycerin at a molar ratio of 1:3, after heating at 450rpm and 60°C for 2 hours, a clear and uniform low-eutectic (3) After mixing the deep eutectic reagent and water uniformly at a volume ratio of 1:1, mix the degreased tea meal with the deep eutectic reagent-water mixture at a mass volume ratio of 1:10, and Ultrasonic power of 400W, microwave power of 150W and microwave-ultrasound for 25min at 40°C were used for filtration, and the filter residue was extracted twice and the filtrate was combined. (4) Add 0.5 times of methanol to the filtrate to precipitate the eutectic reagent, and dissolve tea saponin...

Embodiment 3

[0027] A method for extracting natural high-purity tea saponin, comprising the following steps: (1) drying the tea dregs in a drying oven at 40°C for 12 hours, pulverizing them with a pulverizer, passing through a 60-mesh sieve, and using 3 times the amount of petroleum ether Heating and reflux at 60°C for 1.5 hours, degreasing, filtering, and drying; (2) After mixing L-carnitine and ethylene glycol at a molar ratio of 1:3, heating at 450rpm and 70°C for 1 hour, a clear and uniform (3) After mixing the deep eutectic reagent and water uniformly at a volume ratio of 1:0.8, mix the degreased tea meal with the deep eutectic reagent-water mixture at a mass volume of 1:15 Specific mixing, microwave-ultrasound for 30 min at 300W ultrasonic power, 200W microwave power and 40°C and then filtered, the filter residue was extracted twice and the filtrate was combined. (4) Add 0.8 times of methanol to the filtrate to precipitate the eutectic reagent, and dissolve tea saponin in methanol, c...

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Abstract

The invention discloses an extraction method of natural high-purity tea saponin. The extraction method includes: degreasing tea meal, using an eutectic reagent combined with ultrasonic waves to treat the tea meal, and filtering; adding methanol into filtrate to recycle the eutectic reagent while dissolving tea saponin; loading a sample of an extracted tea saponin solution into a chromatographic column made of macroporous resin, sequentially adopting 10%, 20%, 30%, 40% and 50% alcohol-water solution for elution, collecting eluate of 40% alcohol-water, and drying the eluate to obtain white tea saponin powder higher than 98% in purity. By adopting the method to extract tea saponin from the tea meal, extraction efficiency of tea saponin is improved while consumption of organic solvent is reduced. Tea saponin obtained by the method can be applied in daily chemical products like shampoo, shower gel and facial cleansing milk to enable the products to efficiently remove stain without causing skin injury.

Description

technical field [0001] The invention relates to a method for extracting and purifying active ingredients in daily chemicals, more specifically, the invention relates to a method for extracting natural high-purity tea saponin. Background technique [0002] Daily chemicals such as shampoo, shower gel, facial cleanser, etc. have good degreasing and decontamination capabilities and are widely used by people. At present, daily chemicals are also indispensable daily necessities in life. With the continuous improvement of living standards, people have higher and higher functional requirements for daily chemicals. The survey report shows that 80% of consumers will consider product efficacy when purchasing daily chemical products, among which efficient decontamination and skin care functions are the most selected functions. [0003] The most effective ingredients in traditional cosmetics are surfactants, which play a role in cleaning the skin. However, most shampoos currently on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 林燕
Owner 林燕
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