A kind of rapid propagation method of folk medicinal material Xiaotongcao
A technology of Xiaotongcao and medicinal materials, which is applied in the field of plant tissue culture, can solve the problems of no patent application for tissue culture and rapid propagation of Jingjiehua of the Western Regions, and there is no patent application for Jingjiehua of the Western Regions, etc., and achieves low cost, simple process, and high reproduction coefficient. Effect
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Embodiment 1
[0021] (1) Collection of explants: In the autumn of 2012, when the fruit of Xiyu Jingjiehua was ripe, the mature but uncracked fruit was selected as explants and brought back to the laboratory in time.
[0022] (2) Aseptic sowing: the fruits brought back to the laboratory were washed 3 times with detergent, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 15 minutes, washed 2 times with sterile water, and then rinsed with sterile water. After the bacterial filter paper has blotted the water, put it in 0.1% mercuric chloride solution for disinfection for 20 minutes, rinse it with sterile water for 3 times, blot the water with sterile filter paper, cut the fruit longitudinally with a scalpel, and use sterile tweezers to remove the fruit. The seeds can germinate after being inoculated in the seed germination medium and cultured for 15 days, the germination rate is 87.6%, and the pollution rate is lower than 15%. The seed germination medium is: MS+1.0mg...
Embodiment 2
[0029] (1) Collection of explants: In the autumn of 2013, when the fruit of Xiyu Jingjiehua was ripe, the mature but uncracked fruit was selected as explants and brought back to the laboratory in time.
[0030] (2) Aseptic sowing: the fruits brought back to the laboratory were washed 4 times with detergent, placed in a super-clean workbench and sterilized in 75% ethanol solution for 24 minutes, washed 3 times with sterile water and then rinsed with sterile water. After the bacteria filter paper has blotted the water, put it in 0.1% mercuric chloride solution for disinfection for 25 minutes, rinse it with sterile water for 4 times, blot the water with sterile filter paper, cut the fruit longitudinally with a scalpel, and use sterile tweezers to remove the fruit. The seeds can germinate after being inoculated in the seed germination medium and cultured for 18 days, the germination rate is 85.3%, and the contamination rate is 8.4%. The seed germination medium is: MS+0.8mg / L NAA+2...
Embodiment 3
[0037] (1) Collection of explants: In the autumn of 2014, when the fruit of Xiyu Jingjiehua was ripe, the mature but uncracked fruit was selected as explants and brought back to the laboratory in time.
[0038] (2) Aseptic sowing: the fruits brought back to the laboratory were washed 5 times with detergent, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 30 minutes, washed 3 times with sterile water, and then rinsed with sterile water. After the bacteria filter paper has blotted the water, put it in 0.1% mercuric chloride solution for disinfection for 30 minutes, rinse it with sterile water for 5 times, blot the water with the sterile filter paper, cut the fruit longitudinally with a scalpel, and use sterile tweezers to remove the fruit. The seeds can germinate after being inoculated in the seed germination medium and cultured for 20 days, the germination rate is 83.1%, and the pollution rate is lower than 3%. The seed germination medium is: MS+0....
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