Gelatin-based reverse gel crystal hydrogel three-dimensional bracket, preparation method and application thereof

Abstract

The invention relates to a gelatin-based reverse gel crystal hydrogel three-dimensional bracket, a preparation method and an application thereof. According to the invention, a template micromanufacturing technique and a high-concentration light-induced crosslinking methacrylamide gelatin solution are utilized to manufacture a protein-based three-dimensional bracket with uniform pore interconnection, structure stability and adjustable degradation property. The gelatin-based reverse gel crystal hydrogel three-dimensional bracket can supply excellent cell attachment site and better intercellular interaction of liver cells, has the potential of serving as effective artificial liver platform for screening drugs and also has the potential of a multifunctional platform for same tissue engineering purpose and in vivo application. According to the invention, a highly ordered protein-based inverse opal hydrogel with biological degradability and uniform pore connectivity is prepared; the highly ordered protein-based inverse opal hydrogel can be used for biological application, such as, three-dimensional biological printing, three-dimensional cell culture, drug screening and in vivo transplanting; the gelatin-based reverse gel crystal hydrogel three-dimensional bracket is simple, controllable, regeneratable and economical.

Description

technical field [0001] The invention specifically relates to the technical field of biomaterials, in particular to a gelatin-based inverted colloidal crystal hydrogel three-dimensional scaffold and a preparation method and application thereof. Background technique [0002] The artificially synthesized three-dimensional scaffold mimics the target tissue according to the extracellular matrix composition, mechanical properties, and geometric features, facilitating cell-matrix and cell-cell interactions in vivo. They are highly sought after because cell studies performed on these 3D scaffolds provide physiological information about cell morphology and function compared to 2D substrates. However, the extracellular matrix in vivo is mainly protein-based, highly porous, highly ordered, and characterized by microstructural features. Despite recent advances in 3D printing, microfluidic methods, and electrospinning, it remains a challenge for pure protein-based 3D scaffolds with unif...

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Problems solved by technology

Additionally, most systems use ionic or chemical crosslinkers (calcium chloride, genipal, etc.) for crosslinking, which require longer processing t

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