Preparation method of decalcified bone matrix bone-repairing material

A technology for decalcified bone matrix and bone repair, applied in prosthesis, tissue regeneration, medical science, etc., can solve the problems of poor plasticity, poor polymerization, unsuitable for filling, etc., achieve good swelling capacity and degradation performance, and preparation process The process is simple and the effect of solving irregular bone defects is solved

Inactive Publication Date: 2017-08-18
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DBM is divided into block and powder. Block DBM has poor plasticity, while DBM powder also has the disadvantages of poor polymerization and unsuitable filling operation during operation, and DBM powder implanted in bone defect is easily carried away from the original site by fluid body fluid.

Method used

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  • Preparation method of decalcified bone matrix bone-repairing material
  • Preparation method of decalcified bone matrix bone-repairing material
  • Preparation method of decalcified bone matrix bone-repairing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Add 1 g of sodium alginate to 100 ml of phosphate buffer solution with a pH of 7, stir and mix evenly to obtain a sodium alginate solution with a weight to volume ratio of 1%;

[0030] 2) Add the decalcified bone matrix powder into the sodium alginate solution, stir well to make a suspension with the concentration of the decalcified bone matrix powder at 25 mg / ml, and store it at 4°C for later use;

[0031] 3) Prepare CaCl with a weight concentration of 2% with double distilled water 2 solution, the suspension in step 2) is placed in a cylindrical mold, and CaCl is slowly added dropwise to the suspension 2 solution until the suspension gelled (CaCl 2 The addition amount of the solution is 3.5% of the suspension volume), then take out the strip gel from the mold after standing for 1 hour;

[0032] 4) Freeze-dry the strip-shaped gel. The freeze-drying procedure is as follows: first freeze at -80°C for 12 hours, then immediately transfer to a vacuum freeze-dryer, set ...

Embodiment 2

[0035] 1) Add 0.5 g of sodium alginate to 100 ml of phosphate buffer solution with a pH of 6.5, stir and mix evenly to obtain a sodium alginate solution with a weight to volume ratio of 0.5%;

[0036] 2) Add the decalcified bone matrix powder into the sodium alginate solution, stir well to make a suspension with the concentration of the decalcified bone matrix powder at 20 mg / ml, and store it at 4°C for later use;

[0037] 3) Prepare CaCl with a weight concentration of 1% with double distilled water 2 solution, the suspension in step 2) is placed in a mold, and CaCl is added dropwise to the suspension 2 Solution (CaCl 2 The amount of solution added is 5% of the volume of the suspension), until the suspension gels, and then after standing for 2 hours, the strip gel is taken out from the mold;

[0038] 4) Freeze-dry the strip-shaped gel to obtain the product.

Embodiment 3

[0040] 1) Add 2 g of sodium alginate to 100 ml of phosphate buffer solution with a pH of 8, stir and mix evenly to obtain a sodium alginate solution with a weight-to-volume ratio of 2%;

[0041] 2) Add the decalcified bone matrix powder into the sodium alginate solution, stir well to make a suspension with the concentration of the decalcified bone matrix powder at 50 mg / ml, and store it at 4°C for later use;

[0042] 3) Prepare CaCl with a weight concentration of 3% with double distilled water 2 solution, the suspension in step 2) is placed in a mold, and CaCl is added dropwise to the suspension 2 solution until the suspension gels, then remove the strip gel from the mold after standing for 1 hour;

[0043] 4) Freeze-dry the strip-shaped gel to obtain the product.

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Abstract

The invention discloses a preparation method of a decalcified bone matrix bone-repairing material. The method comprises the following steps: (1) adding alginate into a phosphate buffer solution, and evenly stirring and mixing to obtain 0.5-3% alginate solution; (2) adding decalcified bone matrix powder into the alginate solution, fully stirring, and preparing into 20-100mg/ml suspension; (3) putting the suspension obtained in the step (2) into a mould, dropwise adding a CaCl2 solution into the suspension, standing when the suspension is gelatinized, then taking strip-shaped gel out of the mould, and carrying out freeze drying. The bone-repairing material prepared by the method has good biocompatibility, is capable of inducing the formation of bone tissue components, and enables a new bone to be rapidly formed at the focus position by taking decalcified bone matrix as a support, thus achieving the aims of repairing and filling the bone.

Description

technical field [0001] The invention relates to a bone tissue biological material, in particular to a preparation method of a decalcified bone matrix bone repair material. Background technique [0002] With the aging of the population in our country, the number of cases of spinal degenerative fusion surgery and joint revision surgery is increasing. In addition, there are many cases of bone defects caused by trauma, osteoporosis, bone tumors or bone diseases. These patients need a large number of Filling material for bone tissue repair. The social demand for bone repair materials is increasing day by day, autologous bone cannot meet the dosage requirements, and there are problems such as increasing pain for patients and serious immune rejection of allogeneic bone. Therefore, the tissue engineered bone emerging in recent years has become a research hotspot to solve the problem of bone repair materials. [0003] Decalcified bone matrix (DBM) is a product obtained by decalcify...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/58A61L27/56A61L27/50A61L27/20A61L27/36
CPCA61L27/58A61L27/20A61L27/3608A61L27/365A61L27/50A61L27/56A61L2430/02C08L5/04
Inventor 蔡林谢远龙孙文超邓洲铭张旗
Owner WUHAN UNIV
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