A Nomura strain and its application in the prevention and treatment of Spodoptera pachypterus
A technology of Nomura reinhardtii and Spodoptera frugiperda, applied in the field of biology, can solve the problems of easy generation of drug resistance, Spodoptera frugiperda pollutes the environment, etc., achieves good insecticidal activity and lasting effect, and is easy to develop. Utilize potential, technical effect with obvious effect
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Embodiment 1
[0015] Example 1 Isolation and Identification of the Nomura Reyesi Bacterial Strain Infecting Spodoptera lightoptera
[0016] 1.1 Strain isolation
[0017] The diseased Spodoptera spp. was collected from Shanghai. Dip the inoculation loop to pick up a small amount of epispores from the diseased insects, separate the pathogenic bacteria on a PDA plate containing 0.3% lactic acid by the plate streak separation method, culture at a constant temperature of 25°C for 3-5 days, pick a single colony, and store it in a refrigerator at 4°C for later use.
[0018] 1.2 Test medium
[0019] Potato dextrose agar medium (potato dextrose agar, PDA): boil 200g of peeled potatoes, filter to get the filtrate, glucose 20g, agar powder 16g, add water to 1000mL;
[0020] Sabouraud maltose agar with yeast extract (SMAY): Maltose extract 40g, peptone 10g, yeast powder 10g, agar powder 16g, add water to 1000mL.
[0021] 1.3 Back inoculation test and morphological observation of pathogenic bacteria ...
Embodiment 2
[0037] Example 2 Indoor virulence assay screening against highly virulent strain DT2011N7 of Spodoptera palachoptera
[0038] 2.1 Test materials
[0039] Tested strains: Collect the diseased Spodoptera pachypterae in the field, and isolate the strains according to the method of 1.1 above. Five Nomura strains DT2011N7, XW-DSN6, DT2014-1, DT2014-3, and XW-DSN1 were randomly selected for indoor virulence testing against Spodoptera pallidoptera.
[0040] Insects to be tested: Collect the eggs of Spodoptera pachypterus in the field, and after natural hatching, transfer the larvae to the insect rearing box and place them in a light incubator with light L:D=14:10, humidity 75%, and temperature 25°C , with gramineous turfgrass as feed, artificially reared until the adults emerge and then mate and lay eggs, and the third instar larvae of the next generation are taken as the test insects.
[0041] 2.2 Indoor toxicity test
[0042] Activate the test strain on the SMAY plate. After 10-...
Embodiment 3
[0048] Example 3 The field control effect of DT2011N7 on Spodoptera pallidoptera
[0049] 3.1 Test materials
[0050] DT2011N7 was cultured on SMAY medium at a constant temperature of 25°C for 10-15 days. Spores were eluted with sterile water containing 0.05% Tween 80 to prepare a concentration of 1 × 10 8 Individuals / mL spore suspension, dilute 10 with this spore suspension to prepare 1×10 7 per mL of spore suspension. The control test agent was 1000 times 48% chlorpyrifos EC (Dow AgroSciences).
[0051] 3.2 Test setup
[0052] The field test was carried out in September 2016 on the dwarf Bermuda lawn in Shanghai Chenshan Botanical Garden. 3 treatments (1000 times 48% chlorpyrifos EC dilution, 1×10 8 individual / mL Nomura spore suspension, 1×10 7 Individual / mL Nomura spore suspension), each treatment was repeated 3 times, and each plot was 20m 2 , 9-point checkerboard sampling in the plot, each plot 20cm*20cm, count the number of living insects in the plot. Set water ...
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