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A Nomura strain and its application in the prevention and treatment of Spodoptera pachypterus

A technology of Nomura reinhardtii and Spodoptera frugiperda, applied in the field of biology, can solve the problems of easy generation of drug resistance, Spodoptera frugiperda pollutes the environment, etc., achieves good insecticidal activity and lasting effect, and is easy to develop. Utilize potential, technical effect with obvious effect

Active Publication Date: 2020-05-15
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of Nomura rayii bacterial strain and its application in preventing and treating Spodoptera pleuroptera, and the Nomura bacterium strain and its application in preventing and treating Spodoptera pleuroptera need to solve the problem. In the prior art, the use of chemical pesticides to prevent and control the lawn pest Spodoptera pachypterus pollutes the environment and is prone to produce technical problems of drug resistance

Method used

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  • A Nomura strain and its application in the prevention and treatment of Spodoptera pachypterus
  • A Nomura strain and its application in the prevention and treatment of Spodoptera pachypterus
  • A Nomura strain and its application in the prevention and treatment of Spodoptera pachypterus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation and Identification of the Nomura Reyesi Bacterial Strain Infecting Spodoptera lightoptera

[0016] 1.1 Strain isolation

[0017] The diseased Spodoptera spp. was collected from Shanghai. Dip the inoculation loop to pick up a small amount of epispores from the diseased insects, separate the pathogenic bacteria on a PDA plate containing 0.3% lactic acid by the plate streak separation method, culture at a constant temperature of 25°C for 3-5 days, pick a single colony, and store it in a refrigerator at 4°C for later use.

[0018] 1.2 Test medium

[0019] Potato dextrose agar medium (potato dextrose agar, PDA): boil 200g of peeled potatoes, filter to get the filtrate, glucose 20g, agar powder 16g, add water to 1000mL;

[0020] Sabouraud maltose agar with yeast extract (SMAY): Maltose extract 40g, peptone 10g, yeast powder 10g, agar powder 16g, add water to 1000mL.

[0021] 1.3 Back inoculation test and morphological observation of pathogenic bacteria ...

Embodiment 2

[0037] Example 2 Indoor virulence assay screening against highly virulent strain DT2011N7 of Spodoptera palachoptera

[0038] 2.1 Test materials

[0039] Tested strains: Collect the diseased Spodoptera pachypterae in the field, and isolate the strains according to the method of 1.1 above. Five Nomura strains DT2011N7, XW-DSN6, DT2014-1, DT2014-3, and XW-DSN1 were randomly selected for indoor virulence testing against Spodoptera pallidoptera.

[0040] Insects to be tested: Collect the eggs of Spodoptera pachypterus in the field, and after natural hatching, transfer the larvae to the insect rearing box and place them in a light incubator with light L:D=14:10, humidity 75%, and temperature 25°C , with gramineous turfgrass as feed, artificially reared until the adults emerge and then mate and lay eggs, and the third instar larvae of the next generation are taken as the test insects.

[0041] 2.2 Indoor toxicity test

[0042] Activate the test strain on the SMAY plate. After 10-...

Embodiment 3

[0048] Example 3 The field control effect of DT2011N7 on Spodoptera pallidoptera

[0049] 3.1 Test materials

[0050] DT2011N7 was cultured on SMAY medium at a constant temperature of 25°C for 10-15 days. Spores were eluted with sterile water containing 0.05% Tween 80 to prepare a concentration of 1 × 10 8 Individuals / mL spore suspension, dilute 10 with this spore suspension to prepare 1×10 7 per mL of spore suspension. The control test agent was 1000 times 48% chlorpyrifos EC (Dow AgroSciences).

[0051] 3.2 Test setup

[0052] The field test was carried out in September 2016 on the dwarf Bermuda lawn in Shanghai Chenshan Botanical Garden. 3 treatments (1000 times 48% chlorpyrifos EC dilution, 1×10 8 individual / mL Nomura spore suspension, 1×10 7 Individual / mL Nomura spore suspension), each treatment was repeated 3 times, and each plot was 20m 2 , 9-point checkerboard sampling in the plot, each plot 20cm*20cm, count the number of living insects in the plot. Set water ...

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Abstract

The invention belongs to the field of biological pesticides, and provides a strain of Nomura reinhardtii. The present invention also provides the use of the above-mentioned Nomura reinhardtii strain in controlling Spodoptera frugiperda. The invention overcomes the shortcomings of the method for chemically controlling Spodoptera frugiperda, which pollutes the environment and is easy to produce drug resistance. The lethal time of the strain of the present invention to Spodoptera frugiperda at 25°C is 4.47 days, the corrected control effect of the field control drug after 7 days and 10 days is 78.53% and 89.85%, respectively, and 46.17% after 20 days after the drug. The control effect shows that the strain of the present invention has good insecticidal activity and lasting effect on Spodoptera frugiperda.

Description

Technical field: [0001] The invention belongs to the field of biology, and relates to an insecticidal fungus, in particular to a strain of Nomura spp. and its application in preventing and treating Spodoptera amaranthus. Background technique: [0002] The use of entomopathogenic fungi to control pests is one of the important means of pest control. Compared with traditional chemical control, fungal pathogens have the following advantages: they are safe to non-target organisms (including humans) and the environment, cause the prevalence of insect diseases, and are not easy to produce resistance. Medicinal properties. Therefore, the research and development of fungal insecticides has received extensive attention and attention. [0003] Spodoptera depravata (named Sidemia depravata) is an important Lepidoptera pest that has harmed many kinds of Gramineae turfgrass in recent years, and it is also distributed in Japan and Korea. In my country, the insect has been reported to cau...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 徐颖罗卿权路广亮
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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