A kind of trichoderma fermentation substrate based on monosodium glutamate waste liquid and its application

A technology of fermentation substrate and monosodium glutamate waste liquid, applied in the direction of microorganism-based methods, applications, fertilizers made from biological waste, etc., can solve the problem of high cost of fermentation substrates, achieve low prices, reduce pollution, and achieve the effects of high-efficiency production

Active Publication Date: 2020-09-08
ZHOUKOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the deficiency of the high cost of fermentation substrate in the current production of Trichoderma conidia and the lack of practice of developing the residue derived from monosodium glutamate production waste liquid into Trichoderma microbial fertilizer, and provides a kind of high-yielding Trichoderma conidia and The Trichoderma fermentation substrate that shortens the sporulation cycle and its application solves the environmental pollution caused by the discharge of monosodium glutamate residues into the environment, greatly reduces the cost of Trichoderma conidia fermentation, and improves the efficiency of Trichoderma fermentation. Lay the foundation for further development of Trichoderma monosodium glutamate residue microbial fertilizer

Method used

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  • A kind of trichoderma fermentation substrate based on monosodium glutamate waste liquid and its application
  • A kind of trichoderma fermentation substrate based on monosodium glutamate waste liquid and its application
  • A kind of trichoderma fermentation substrate based on monosodium glutamate waste liquid and its application

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Effect test

Embodiment 1

[0029] A Trichoderma fermentation substrate based on monosodium glutamate waste liquid, concentrated with monosodium glutamate waste liquid (glutamic acid mother liquor after monosodium glutamate production), and then adding inorganic nitrogen source, phosphorus source and potassium source, so that the composition of the final fermentation substrate is: Nitrogen 6-7%, phosphorus 2-3%, potassium 0.5-1.0%, organic matter ≥ 35%, pH=6.5-7.0, moisture ≤ 30%. The used glutamic acid mother liquor characteristic of the present invention is: dry basis is 22%, and the composition after being dried is: nitrogen 14%, phosphorus 1.0%, potassium 1.8%, organic matter 48%, Pomi degree is 17 o Be, pH 3.0. The inorganic nitrogen source is ammonium sulfate and ammonium phosphate, the phosphorus source is ammonium phosphate, and the potassium source is potassium chloride.

[0030] Add 30-35 times the mass of distilled water to the above-mentioned fermentation substrate to obtain a liquid medium,...

Embodiment 2

[0035] Obtaining Trichoderma fermentation substrate: a Trichoderma fermentation substrate based on MSG waste liquid, concentrated with MSG waste liquid (glutamic acid mother liquor after MSG production), and then adding ammonium sulfate, ammonium phosphate and potassium chloride and organic matter, The composition of the final fermentation substrate is: 6.4% nitrogen, 2.4% phosphorus, 0.7% potassium, 35% organic matter, pH=6.9, and 30% moisture. The used glutamic acid mother liquor characteristic of the present invention is: dry base is 22%, and the composition after being dried is: total nitrogen 14%, phosphorus 1.0%, potassium 1.8%, organic matter 48%, Pomi degree is 17 o Be, pH 3.0.

[0036] Preparation of Trichoderma fermentation medium: 30g of culture medium was added to distilled water to make the volume to 1000mL, then 20g of agar was added, and 20mL of medium was poured into a 90mm petri dish to prepare a solid fermentation medium plate.

[0037] Trichoderma inoculati...

Embodiment 3

[0053] Obtaining Trichoderma fermentation substrate: a Trichoderma fermentation substrate based on MSG waste liquid, concentrated with MSG waste liquid (glutamic acid mother liquor after MSG production), and then adding ammonium sulfate, ammonium phosphate and potassium chloride and organic matter, The composition of the final fermentation substrate is: 6% nitrogen, 2% phosphorus, 0.5% potassium, 35% organic matter, pH=6.5, and 30% moisture. The used glutamic acid mother liquor characteristic of the present invention is: dry basis is 22%, and the composition after being dried is: nitrogen 14%, phosphorus 1.0%, potassium 1.8%, organic matter 48%, Pomi degree is 17 o Be, pH 3.0.

[0054] Preparation of Trichoderma fermentation medium: 33.3 g of culture medium was added with distilled water to make the volume to 1000 mL, then 18 g of agar was added, and 20 mL of medium was poured into a 90 mm petri dish to prepare a solid fermentation medium plate.

[0055] Trichoderma inoculati...

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Abstract

The invention discloses a trichoderma fermentation substrate based on aginomoto waste liquid. A preparation method of the trichoderma fermentation substrate is characterized by concentrating glutamic acid mother liquid obtained after producing aginomoto, then adding inorganic nitrogen source, phosphorus sources, potassium resources and organic matters, and enabling the final fermentation substrate to be prepared from 6-7% of nitrogen, 2-3% of phosphorus, 0.5-1.0% of potassium, greater than or equal to 35% of organic matters and less than or equal to 30% of water, wherein the pH value is 6.5-7.0. The glutamic acid mother liquid is prepared from the following dry substrate components: 14% of nitrogen, 1.0% of phosphorus, 1.8% of potassium and 48% of organic matters, wherein the Baume degree is 17 degrees Be, and the pH value is 3.0. The trichoderma is added into the fermentation substrate and has synergistic effect on exertion of fertilizer efficiency of a plant growth substrate; the aginomoto residue in the production of aginomoto can be changed from waste into wealth; the pollution to the environment caused by emission of the aginomoto residue in the environment is reduced; the yield of trichoderma conidia is increased; the conidium production time is shortened; the trichoderma conidia can be efficiently produced by the aginomoto residue.

Description

technical field [0001] The invention relates to a method for utilizing monosodium glutamate waste liquid, in particular to a trichoderma fermentation substrate based on monosodium glutamate waste liquid and its application. Background technique [0002] According to the survey, there are more than 200 large and small monosodium glutamate production enterprises in my country at present, with a production scale of 3 million tons per year. According to analysis, for every ton of monosodium glutamate produced, there will be 3-4 tons of high-concentration organic wastewater, from which 200-250Kg of monosodium glutamate residue can be extracted. In the past, these wastewaters were usually discharged directly, but such highly nutritious organic water is harmful to water areas. And ecological environment pollution is immeasurable. After separation and recycling of these wastewater, 200,000 tons of MSG residues can be obtained. These MSG residues can be made into organic fertilizers...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05G3/00C12R1/885
CPCC05D1/00C05G3/00C12N1/14C05F5/008C05F11/08
Inventor 张福丽窦恺张杰韩洪军高立栋田晓燕井金峰刘继东樊小宁刘畅杨同文陈龙
Owner ZHOUKOU NORMAL UNIV
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