Preparation method capable of realizing aspergillus niger spore multiplication through carrier adsorption and producing xerospore powder of aspergillus niger

Abstract

The invention discloses a preparation method capable of realizing aspergillus niger spore multiplication through carrier adsorption and producing xerospore powder of aspergillus niger. The preparation method comprises steps as follows: corn and bran are mixed in the ratio of 7:3, the corn and bran mixture and water are mixed in the ratio of the total weight of solids to the volume of water being 1:5, then, high-temperature-resistant amylase is added, the mixture is boiled at 100 DEG C for 30 min and filtered, and a culture solution of aspergillus niger spores is obtained. Crushed corncobs in the volume of 1 cm<3> is mixed with the culture solution of aspergillus niger in the weight-to-volume ratio being 1:3.5, and the mixture is put in a solid-state fermentation tank, sterilized at 121 DEG C for 20 min, cooled to 37 DEG C and inoculated with 104-105 CFU / mL aspergillus niger spores on the basis of the volume of the culture solution of aspergillus niger. The solid-state fermentation tank is kept at the temperature of 37 DEG C, the mixture is cultured for 120 h, a compound protectant is added after the aspergillus niger spores are diluted, the volume ratio of a spore suspension to the compound protectant is 2:3, the balancing time lasts for 40 min, vacuum drying is performed, and xerospore powder of aspergillus niger is collected. By means of the preparation method, the survival rate of aspergillus niger spore powder is increased, and the storage period of strains is prolonged. Meanwhile, a compound dryer adding method consumes little energy, and the preparation method adopts a simple process and is low in cost and more suitable for large-scale production.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for adsorbing Aspergillus niger spores to proliferate on a porous carrier, a drying composite protective agent for Aspergillus niger spore powder and a drying method. Background technique [0002] In the field of industrial fermentation, the expansion and cultivation of strains is an important part of the fermentation process. Aspergillus niger is an extremely important industrial microorganism, and it is a production bacterium of many fermentation products, especially organic acids, enzyme preparations, antibiotics and other industries, used to produce citric acid, malic acid, gallic acid, itaconic acid, malic acid and other organic acids , It can also be used to produce more than 30 kinds of enzyme preparations such as glucoamylase, acid protease and pectinase. There are as many as 26 kinds of food-use enzyme preparations derived from Aspergillus niger s...

AI Technical Summary

Problems solved by technology

However, a large number of studies have shown that most enzymes in microbial cells will be greatly affected during the freeze-drying process, but there is no good way to prevent the activity of these enzymes from changing during freeze-drying.

On the other hand, freeze-drying has many disadvantages at the same time: the freeze-drying process is complicated to operate, the cost of equipment is high, the drying time is long, and the process conditions are relatively strict, which affects the industrialization and commercialization of probiotics.

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