Preparation method capable of realizing aspergillus niger spore multiplication through carrier adsorption and producing xerospore powder of aspergillus niger

A technology of Aspergillus niger spores and Aspergillus niger, which is applied to the dry compound protective agent of Aspergillus niger spore powder and the field of drying and porous carrier adsorption of Aspergillus niger spore proliferation, which can solve the problems of high instrument cost, long drying time, and activity change, and achieve The effect of large number of proliferation and fast growth

Pending Publication Date: 2017-08-18
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of studies have shown that most enzymes in microbial cells will be greatly affected during the freeze-drying process, but there is no good way to prevent the activity of these enzymes from changing during freeze-drying.
On the other hand, freeze-dryin

Method used

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  • Preparation method capable of realizing aspergillus niger spore multiplication through carrier adsorption and producing xerospore powder of aspergillus niger
  • Preparation method capable of realizing aspergillus niger spore multiplication through carrier adsorption and producing xerospore powder of aspergillus niger
  • Preparation method capable of realizing aspergillus niger spore multiplication through carrier adsorption and producing xerospore powder of aspergillus niger

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] A preparation method for aspergillus niger spore proliferation by carrier adsorption;

[0092] (1) Preparation of Aspergillus niger spore proliferation culture medium:

[0093] Mix corn and bran in a ratio of 7:3 by weight, and mix corn bran with water according to the volume ratio of the total weight of solids to water as 1:5, and mix amylase with 12 units per gram of corn weight Add it to the above mixture to liquefy, boil at 100°C for 30 minutes, filter, and the filtrate will be dissolved to the original volume of water added to obtain the culture solution of Aspergillus niger spores;

[0094] (2) Preparation of Aspergillus niger spore culture by solid-state fermenter carrier:

[0095] will be crushed into a volume of 1cm 3 The corn cob cores were mixed with the above-mentioned Aspergillus niger culture solution at a weight-to-volume ratio of 1:3.5, put into a solid-state fermenter, sterilized at 121°C for 20 minutes, cooled to 37°C and inserted with Aspergillus ni...

Embodiment 2

[0101] A preparation method for aspergillus niger spore proliferation by carrier adsorption;

[0102] (1) Preparation of Aspergillus niger spore proliferation culture medium:

[0103] Mix corn and bran in a ratio of 7:3 by weight, and mix corn bran with water according to the volume ratio of the total weight of solids to water as 1:5, and mix amylase with 12 units per gram of corn weight Add it to the above mixture to liquefy, boil at 100°C for 30 minutes, filter, and the filtrate will be dissolved to the original volume of water added to obtain the culture solution of Aspergillus niger spores;

[0104] (2) Preparation of Aspergillus niger spore culture by solid-state fermenter carrier:

[0105] will be crushed into a volume of 1cm 3 The corn cob cores were mixed with the above-mentioned Aspergillus niger culture solution at a weight-to-volume ratio of 1:3.5, put into a solid-state fermenter, sterilized at 121°C for 20 minutes, cooled to 37°C and inserted with Aspergillus ni...

Embodiment 3

[0112] Aspergillus niger spore fermentation production citric acid prepared by the present invention:

[0113] Citric acid is an important organic acid, widely used in food, medicine, chemical industry and other industries It is the first acid agent used, accounting for more than 60% of the amount of food acidulant. The dry spore powder provided by the invention is used for inoculation and fermentation of citric acid factories to produce citric acid. And determine the optimal inoculation amount.

[0114] (1) Preparation of fermentation medium:

[0115] Corn liquid with dregs provided by the cooperative citric acid company. Add the slag-bearing corn liquid into the fermenter with a liquid volume of 8%, and sterilize it at 121°C for 20 minutes.

[0116] (2) Production of citric acid:

[0117] The dry spore powder provided by the present invention is inserted into the fermenter, and the inoculum amount is 6%, 8%, 10%, 12%, and 14%, respectively, and the inoculum amount is ca...

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Abstract

The invention discloses a preparation method capable of realizing aspergillus niger spore multiplication through carrier adsorption and producing xerospore powder of aspergillus niger. The preparation method comprises steps as follows: corn and bran are mixed in the ratio of 7:3, the corn and bran mixture and water are mixed in the ratio of the total weight of solids to the volume of water being 1:5, then, high-temperature-resistant amylase is added, the mixture is boiled at 100 DEG C for 30 min and filtered, and a culture solution of aspergillus niger spores is obtained. Crushed corncobs in the volume of 1 cm<3> is mixed with the culture solution of aspergillus niger in the weight-to-volume ratio being 1:3.5, and the mixture is put in a solid-state fermentation tank, sterilized at 121 DEG C for 20 min, cooled to 37 DEG C and inoculated with 104-105 CFU/mL aspergillus niger spores on the basis of the volume of the culture solution of aspergillus niger. The solid-state fermentation tank is kept at the temperature of 37 DEG C, the mixture is cultured for 120 h, a compound protectant is added after the aspergillus niger spores are diluted, the volume ratio of a spore suspension to the compound protectant is 2:3, the balancing time lasts for 40 min, vacuum drying is performed, and xerospore powder of aspergillus niger is collected. By means of the preparation method, the survival rate of aspergillus niger spore powder is increased, and the storage period of strains is prolonged. Meanwhile, a compound dryer adding method consumes little energy, and the preparation method adopts a simple process and is low in cost and more suitable for large-scale production.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for adsorbing Aspergillus niger spores to proliferate on a porous carrier, a drying composite protective agent for Aspergillus niger spore powder and a drying method. Background technique [0002] In the field of industrial fermentation, the expansion and cultivation of strains is an important part of the fermentation process. Aspergillus niger is an extremely important industrial microorganism, and it is a production bacterium of many fermentation products, especially organic acids, enzyme preparations, antibiotics and other industries, used to produce citric acid, malic acid, gallic acid, itaconic acid, malic acid and other organic acids , It can also be used to produce more than 30 kinds of enzyme preparations such as glucoamylase, acid protease and pectinase. There are as many as 26 kinds of food-use enzyme preparations derived from Aspergillus niger s...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N11/02C12R1/685
CPCC12N3/00C12N11/02
Inventor 王德培黄娟
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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