High Salt Toothpaste and How to Use It
A technology of toothpaste, use, applied in the field of toothpaste composition
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Embodiment 1
[0071] Example 1 - Micro-robustness testing
[0072] The Micro Robustness Test (MRT) is a quantitative measure of the ability of a composition to withstand microbial challenge. Thus, the result is an assessment of the antimicrobial efficacy of the composition against a pool of microorganisms.
[0073] The following eleven microorganisms were included in the microbial pool: Burkholderia cepacia, Enterobacter cloacae, Escherichia coli, Klesiella oxytoca , Klebsiella pneumoniae, Serratia marcescens, Providencia rettgeri, Pseudomonas aeruginosa, Pseudomonas putida Pseudomonas putida, Staphylococcus aureus and Staphylococcus saprophyticus.
[0074] The solution level for total microorganisms is 10 7 cfu / ml. Use 10 at 60 minute intervals 7 Bacterial challenge samples from the above microbial pool were challenged three times. After 4, 6 and 24 hours, aliquots were tested to measure the log reduction in bacteria levels. Table 1 below shows the results of the antimicrobial test...
Embodiment 2
[0090] Example 2 - Biofilm Reduction Test
[0091] METHODS: Plaques were collected from 4 healthy volunteers and pooled together to form an inoculum. The O.D. of the inoculum was matched to an absorbance of 0.3 at 610 nm. Sterile HAP discs were incubated anaerobically at 37° C. with 1 ml of sterile artificial saliva (with 0.01% sucrose) and 1 ml of confluent saliva in a 24-well microplate for 24 hours. Freshly prepared treatment solutions (1 part toothpaste of Example 1 above to 2 parts sterile distilled water) were added to the wells and allowed to contact the HAP discs for 10 minutes.
[0092] The liquid phase was removed and replaced with 2 ml sterile artificial saliva. Discs of each control and test dentifrice were treated in triplicate for 8 days. At 2, 4 and 8 day intervals, discs were aseptically collected and transferred to thioglycolate medium previously reduced in strength by half. 100 ml of 10-4, 10-5 and 10-6 dilutions from each disc were plated in duplicate ...
Embodiment 3
[0124] Embodiment 3-in vitro antibacterial efficacy research
[0125] Method: Four sample toothpaste compositions were selected for the study
[0126] Sample 1: NCC base with 10% sodium chloride and 0.2% zinc citrate
[0127] Sample 2: NCC base with 10% sodium chloride
[0128] Sample 3: Silica base with 10% sodium chloride and 0.2% zinc citrate
[0129] Sample 4: Silica base with 10% sodium chloride
[0130] Test samples were serially diluted 5-fold in sterile water. A 4.5 mL sample was used for each time interval. The inoculum was prepared in the selected medium and adjusted to 0.5 McFarland standard (Actinomycetes viscous ATCC 43146, Streptococcus oralis ATCC 35037, Veillonella parvula ATCC 17745, Lactobacillus casei ATCC 334 and Fusobacterium nucleatum ATCC 10953). Each sample and control was inoculated with 0.5 mL of the prepared medium. At the end of the selected contact time, pipette 0.5 of the inoculum sample into 4.5 mL of D / E Neutralization Broth. Vortex th...
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