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Strain bacillus subtilis SEM-9 from silkworm excrement and application thereof

A technology of SEM-9 and silkworm excrement, applied in the field of microorganisms, can solve the problems of chemical residues, soil degradation pathogens, environmental pollution, etc., and achieve the effects of improving yield and quality, good high temperature resistance, and good salt and alkali resistance

Active Publication Date: 2017-09-12
GUANGDONG GEOLONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding cycle of disease-resistant varieties is long and easy to lose resistance. Although chemical drugs have the advantages of high control efficiency, fast speed, broad insecticidal and antibacterial spectrum, low cost and simple use, long-term use of chemical agents can easily lead to soil degradation and pathogenic bacteria. Drug resistance, causing serious ecological and agricultural product safety problems such as rampant secondary diseases, pesticide residues, and environmental pollution

Method used

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  • Strain bacillus subtilis SEM-9 from silkworm excrement and application thereof
  • Strain bacillus subtilis SEM-9 from silkworm excrement and application thereof
  • Strain bacillus subtilis SEM-9 from silkworm excrement and application thereof

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Experimental program
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Effect test

Embodiment 1

[0032] Isolation and Identification of Bacillus subtilis SEM-9 Strain

[0033] (1) Sample dilution: take a sample from the fermented and decomposed silkworm excrement compost, weigh 5g and dissolve it in a triangular flask with 45ml sterile water, shake it with a 150rpm oscillator to form a uniform 10 -1 diluent, and then use a 1ml pipette gun to draw 1ml of the diluent into a test tube filled with 9ml sterile water, and shake it well to make 10 -2 sample diluent, and sequentially diluted to 10 -6 .

[0034] (2) Medium preparation:

[0035] Inorganic Phosphorus Solution Screening Medium: Glucose 10.0g, Ca 3 (PO4) 2 5.0g, (NH4) 2 SO 4 0.5g, NaCl0.2g, KCl0.2g, MgSO 4 ·7H 2 O 0.1g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 0.03g of 4H2O, 0.5g of yeast powder, 1000mL of distilled water, pH 6.8-7.2, and sterilize for 20 minutes;

[0036] Potato medium: 200 grams of potatoes, 20 grams of glucose, 18 grams of agar, 1000 ml of distilled water, pH 6.8 to 7.2;

[0037] NA medium: 10g ...

Embodiment 2

[0055] Preparation of Bacillus subtilis (Bacillus subtilis) SEM-9 bacterial liquid, bacterial powder and silkworm excrement microbial fertilizer:

[0056] (1) Activated bacteria

[0057] Activate the Bacillus subtilis (Bacillus subtilis) SEM-9 strain, pick colonies from the slope and streak culture on the NA solid medium, the culture condition is 37°C, after SEM-9 grows on the NA solid medium for 18-24h, Pick a single colony for the preparation of the primary seed solution;

[0058] (2) Preparation of primary seed solution

[0059] Select a single colony on the NA solid medium and inoculate it in the first-level seed shaker flask. The seed shake flask was cultured at 37°C and 200r / min for 14 hours to obtain the first-grade seed liquid;

[0060] (3) Preparation of secondary seed solution

[0061] The medium in the seed tank is still NB liquid medium, sterilized at 121°C for 20 minutes, put the first-grade seed liquid into the seed tank, and the inoculum size is 0.7-1% (V / V)...

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Abstract

The invention belongs to the technical field of microbes, and discloses a strain bacillus subtilis SEM-9 from silkworm excrement and application thereof. The strain bacillus subtilis SEM-9 is collected in the Guangdong Microbial Culture Collection Center (GDMCC) on May 10, 2017; the collection number is GDMCC NO. 60169. The strain bacillus subtilis SEM-9 provided by the invention is from the silkworm excrement, is an autogenous bacterium in a silkworm-excrement composting process, is good in the cooperativity with silkworm-excrement organic fertilizer, can efficiently inhibit the generation of soil-borne diseases of blight and the like of a crop, and achieves the effects of preventing and treating a disease of the crop and improving the disease and insect pest resistance of the crop; therefore, the yield and the quality of the crop is improved. The strain bacillus subtilis SEM-9 can be used for preparing an antagonistic fusarium microbial bacterial agent or silkworm-excrement microbial bacterial fertilizer.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacterial strain Bacillus subtilis SEM-9 derived from silkworm excrement and its application. Background technique [0002] Fusarium wilt is a devastating soil-borne disease known as the "cancer" of plants. The pathogen causing the disease is mainly Fusarium, which has a wide range of hosts and can cause more than 100 kinds of plant diseases. For a long time, people's methods of disease prevention and control mainly include crop rotation, breeding of disease-resistant varieties, chemical drug control and biological control. The breeding cycle of disease-resistant varieties is long and easy to lose resistance. Although chemical drugs have the advantages of high control efficiency, fast speed, broad insecticidal and antibacterial spectrum, low cost and simple use, long-term use of chemical agents can easily lead to soil degradation and pathogenic bacteria. Dr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C05G3/00C05G3/04C05G3/02C12R1/125C05G3/60C05G3/80
CPCA01N63/00C05D3/02C05G3/00C12N1/20C05G3/60C05G3/80C12N1/205C12R2001/125C05F1/005C05F3/00C05F5/002C05F11/08
Inventor 支胡钰吴日章代燕丽林李华吉亮
Owner GUANGDONG GEOLONG BIOTECH
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