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Preparation method and application of antifreeze glycopeptides based on fructooligosaccharide modification

A technology of fructooligosaccharide and antifreeze polypeptide, which is applied in food processing and biological fields, can solve the problems of cell damage, cell survival rate, destruction, lethality, etc., and achieve the effects of low cost, increased functionality, and improved antifreeze activity.

Inactive Publication Date: 2017-09-19
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, studies have found that the recrystallization inhibitory activity plays a crucial role in the cryoprotective effect of antifreeze proteins. Biological materials (such as cells, blood, tissues) are destructive or even lethal, mainly because when the freezing temperature is lower than the thermal hysteresis active temperature range, the ice crystals grow uncontrollably and rapidly transform into needle-like structures, which in turn leads to cell damage and decrease in cell viability after thawing

Method used

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  • Preparation method and application of antifreeze glycopeptides based on fructooligosaccharide modification
  • Preparation method and application of antifreeze glycopeptides based on fructooligosaccharide modification

Examples

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Effect test

Embodiment 1

[0022] A kind of preparation of antifreeze glycopeptide based on fructooligosaccharide modification is carried out according to the following method:

[0023] (1) Mix the antifreeze polypeptide powder and fructooligosaccharide according to the mass ratio between antifreeze polypeptide powder (derived from pig skin) and fructooligosaccharides at a ratio of 1:2.7, and then dissolve them in phosphate buffer solution with pH 7.6 ( 2.6 mM KH 2 PO 4 , 32.5 mM K 2 PO 4 ), the final concentration of the solution was 10% (w / v), stirred for 15 min to fully dissolve;

[0024] (2) The dissolved antifreeze polypeptide and fructooligosaccharide mixture were reacted at 77 °C and pH 8.74 for 67 min; after the reaction was completed, they were immediately cooled in ice water (0 °C) for 20 min to terminate the reaction. The resulting solution was freeze-dried to obtain freeze-dried antifreeze glycopeptide powder.

Embodiment 2

[0026] A kind of preparation of antifreeze glycopeptide based on fructooligosaccharide modification is carried out according to the following method:

[0027] (1) Mix the antifreeze polypeptide powder and fructooligosaccharide according to the mass ratio between antifreeze polypeptide powder (derived from cowhide) and fructooligosaccharides at a ratio of 1:1, and then dissolve them in a phosphate buffer solution with pH 7.8 (2.6 M KH 2 PO 4 , 32.5 mM K 2 PO 4 ), the final concentration of the solution was 5% (w / v), stirred for 10 min to fully dissolve;

[0028] (2) The dissolved antifreeze polypeptide and fructooligosaccharide mixture were reacted at 60 °C and pH 8.0 for 60 min; after the reaction was completed, immediately cooled in ice water (0 °C) for 15 min to terminate the reaction. The resulting solution was freeze-dried to obtain freeze-dried antifreeze glycopeptide powder.

Embodiment 3

[0030] A kind of preparation of antifreeze glycopeptide based on fructooligosaccharide modification is carried out according to the following method:

[0031] (1) Mix the antifreeze polypeptide powder and fructooligosaccharide according to the mass ratio between antifreeze polypeptide powder (derived from fish skin) and fructooligosaccharides at a ratio of 1:5, and then dissolve them in phosphate buffer solution with pH 8.0 ( 2.6 mM KH 2 PO 4 , 32.5 mM K 2 PO 4 ), the final concentration of the solution was 15% (w / v), stirred for 20 min to fully dissolve;

[0032] (2) The dissolved antifreeze polypeptide and fructooligosaccharide mixture were reacted at 80 °C and pH 9.0 for 120 min; after the reaction was completed, immediately cooled in ice water (0 °C) for 30 min to terminate the reaction. The resulting solution was freeze-dried to obtain freeze-dried antifreeze glycopeptide powder.

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Abstract

The invention discloses a preparation method and application of antifreeze glycopeptides based on fructooligosaccharide modification, and belongs to the technical field of biology and food processing. The fructooligosaccharide carbohydrate chain is introduced into antifreeze peptide from different sources by a glycosylation grafting means to synthesize the antifreeze glycopeptides. The antifreeze glycopeptides have good antifreeze functions, and can be applied to low-temperature freeze preservation of cells, tissues, organs, microorganisms and frozen food. The freezing survival rate of the cells, the tissues and the microorganisms added with the antifreeze glycopeptides is obviously improved; when the antifreeze glycopeptides are applied to freezing preservation food, the stability of the regional quality of the freezing food can be well maintained; the food quality deterioration caused by freezing damage is obviously reduced.

Description

technical field [0001] The invention specifically relates to a preparation method and application of an antifreeze glycopeptide based on fructooligosaccharide modification, and belongs to the technical fields of biology and food processing. Background technique [0002] Antifreeze protein, commonly known as ice structural protein, also known as heat hysteresis protein, was first discovered by Devries in 1969 in a polar fish in the Antarctic Channel ( Trematomus borchgrevinki ) found in blood. Antifreeze proteins have cryoprotective activity and can be used as cryoprotectants in medicine, biology, and food processing. For example, if antifreeze protein is applied to the cryopreservation of tissues, cells and microorganisms, it can significantly improve its freezing survival rate; antifreeze protein can reduce the quality deterioration caused by ice crystals and recrystallization of frozen food during low-temperature storage, Improves the stability of frozen foods. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K14/46C07K1/107C12N1/04A01N1/02A23L3/375
CPCC07K14/47A01N1/0221A23L3/375C07K14/461C12N1/04
Inventor 汪少芸陈旭张凌拓王文龙蔡茜茜方卫东
Owner FUZHOU UNIV
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