Processing method of allergy relieving shellfish food meat paste
A processing method and shellfish technology, which is applied in the field of desensitized shellfish paste processing, can solve the problems of inedible shellfish and aquatic products, and achieve the effects of good bacteria reduction effect, tender taste, and convenient eating
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Embodiment 1
[0040] An original flavor desensitized clam mashed potato, comprising the following steps:
[0041] (1) Raw material pretreatment: Fresh and alive clams are temporarily raised to vomit sand and sterilized with ozone; the time for temporary raising to vomit sand is 14 hours, and the ozone generator is passed through the temporary raising pond to sterilize for 10 minutes; the initial bacterial count is reduced;
[0042] (2) Ultra-high pressure shelling: the clams obtained in step (1) are cleaned, treated under ultra-high pressure at room temperature, the pressure is 400MPa, the pressure holding time is 10min, the pressure is released, and the shells are peeled to get the shellfish;
[0043] (3) Ultrasound combined with enzymatic desensitization treatment: add 2 times of pure water to the clam meat obtained in step (2), at room temperature, ultrasonic ultrasonic 1h, frequency 40kHz, power 500W; adjust the temperature to 50 ° C, the natural pH value ( 6.5-7), add 5‰ (w / w) compound...
Embodiment 2
[0050] A kind of desensitization flower clam yam mud, comprises the following steps:
[0051] (1) Raw material pretreatment: live clams are temporarily raised to spit sand and sterilized with ozone; the time for temporary sand spit is 16 hours, and the ozone generator is passed through the temporary cultivation pool to sterilize for 15 minutes; the initial number of bacteria is reduced;
[0052] (2) Ultra-high pressure shelling: the clam obtained in step (1) is cleaned, treated under ultra-high pressure at room temperature, the pressure is 500MPa, the pressure holding time is 10min, the pressure is released, and the shell is peeled to get the shellfish;
[0053](3) Ultrasound combined with enzymatic desensitization treatment: add 3 times pure water to the clam meat obtained in step (2), at room temperature, ultrasonic ultrasonic 2h, frequency 40kHz, power 400W; adjust the temperature to 55 ° C, the natural pH value ( 6.5-7), add 3‰ (w / w) compound protease to hydrolyze for 3 ho...
Embodiment 3
[0064] The parameters of the processing method of the sample in Comparative Example 3 refer to Example 1, except that the step (3) is not processed. The extraction and assay method of embodiment 3 shellfish meat allergen:
[0065] Mollusc aquatic products include clams, oysters, clams, etc. The meat is very delicious, but some people have allergies after eating it. Some studies have shown that there is an allergenic protein in molluscs --- tropomyosin, which mainly exists in the muscle fibers of molluscs, and people will also have allergies after eating them.
[0066] The content of tropomyosin was determined by ELISA kit, and tropomyosin was used as an index of shellfish allergen.
[0067] Described standard curve is measured and obtained with standard substance, and the standard curve obtained (with tropomyosin as standard product), the regression equation through calculation is Y=0.0248X+0.062, correlation coefficient R2=0.999, where Y represents the absorbance value, and...
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