Standard sample of salmonella in milk powder and preparation method of standard sample of salmonella in milk powder

A Salmonella, standard sample technology, applied in biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve problems affecting quality control, short sample shelf life, poor sample uniformity and stability, etc.

Active Publication Date: 2017-10-10
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the target bacteria are prone to change (reproduction and death, etc.) in the prepared Salmonella sample, it will l...

Method used

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  • Standard sample of salmonella in milk powder and preparation method of standard sample of salmonella in milk powder
  • Standard sample of salmonella in milk powder and preparation method of standard sample of salmonella in milk powder
  • Standard sample of salmonella in milk powder and preparation method of standard sample of salmonella in milk powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Salmonella standard sample in milk powder, comprises target bacterium and background flora, and described target flora is Salmonella (Salmonella), and described background flora consists of Bacillus cereus (Bacillus cereus), Staphylococcus aureus (Staphylococcus aureus), cloacae Composition of Enterobacter cloacae and Enterobacter aerogenes.

[0027] The sample is based on trehalose, skimmed milk powder and sterile water, wherein the volume fraction of trehalose is 12%, and the volume fraction of skimmed milk powder is 15%.

[0028] The target concentration of target bacteria in the sample is 10 2 ~10 3 CFU / mL, the target concentration of background flora is 10 3 CFU / mL.

Embodiment 2

[0030] Such as figure 1 As shown, a method for preparing a Salmonella standard sample in milk powder in Example 1 includes four steps of sample addition bacterial strain selection, preparation of lyoprotectant, sample freeze-drying, sample homogeneity and stability test, specifically Proceed as follows:

[0031] 1. Selection of sample addition strains

[0032] According to target bacteria: Salmonella, background flora: Bacillus cereus, Staphylococcus aureus, Enterobacter cloacae, Enterobacter aerogenes composition selection criteria Strains, all standard strains are purchased from institutions designated by the government, and a strain certificate is attached to ensure the traceability of the strains.

[0033] 2. Preparation of lyoprotectant

[0034] The sample was prepared with trehalose, skimmed milk powder and sterile water as the matrix (volume fraction: trehalose 12%, skimmed milk powder 15%) to prepare a lyoprotectant.

[0035] 3. Sample freeze-drying

[0036] (1) S...

Embodiment 3

[0073] Each step of the preparation method of the Salmonella standard sample in milk powder described in this embodiment is all the same as in Example 2, and the different technical parameters are: the bacterial strain culture medium is selected from a nutrient agar slant medium; during the preparation of the bacterial suspension, the target The concentration of the target bacteria in the bacterial suspension was 5×10 3 CFU / mL preparation, the concentration of each background bacteria in the background flora suspension was prepared according to 10CFU / mL; the freeze-drying process was 52h.

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Abstract

The invention belongs to the field of quality control in microbial detection and particularly relates to a standard sample of salmonella in milk powder and a preparation method of the standard sample of the salmonella in the milk powder. The standard sample of the salmonella in the milk powder comprises a target bacterium and background flora, wherein the target bacterium is the salmonella; the background flora comprises Bacillus cereus, Staphylococcus aureus, Enterobacter cloacae and Enterobacter aerogenes. The standard sample meets requirements for uniformity and stability, the stability of the standard sample can be guaranteed to the greatest extent, and a perfect preparation technology for the standard sample is formed by starting from meeting special transport conditions by means of research in special process, stability and uniformity and the like, is suitable for preparing the standard sample, meeting the requirements for uniformity and stability, of the salmonella in the milk powder and is applicable to quality control, method validation and other purposes in a laboratory.

Description

technical field [0001] The invention belongs to the quality control field of microbiological detection, and in particular relates to a standard sample of Salmonella in milk powder and a preparation method thereof. Background technique [0002] The field of microbiological testing of milk powder is very specific. At present, there is no precedent for the preparation of milk powder microbiological standard samples and large-scale production by institutions at home and abroad, which is still a blank field. At present, although some institutions prepare standard samples of microorganisms, the uniformity and stability of the standard samples can hardly meet the requirements, and transportation is extremely difficult; or the standard samples are pure strains containing only target bacteria, which are far from the actual samples Meet the needs of daily laboratory quality control. [0003] Salmonella is the primary pathogen of ingestion-acquired foodborne bacterial gastroenteritis...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12N1/20C12N1/04C12R1/01C12R1/445C12R1/085
CPCC12N1/04C12N1/20C12Q1/10
Inventor 赵红阳卢行安陶德华王鸣雨刘汉霞王伟孙英健韩青
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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