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Glucose oxidase screen-printed electrode for online detection and preparation method thereof

A technology of screen-printing electrodes and oxidase screen, applied in biochemical equipment and methods, measuring devices, electrochemical variables of materials, etc., can solve the problems that hinder the online analysis of glucose biosensors, difficult to batch, industrialized production, and fermentation broth components. Complexity and other problems, to achieve the effect of simple and cheap synthesis equipment, short synthesis time and ideal reproducibility

Active Publication Date: 2017-10-10
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Market-oriented glucose biosensors are facing problems such as difficulty in multiple and repeated analysis tests, or difficulty in batch and industrial production
At the same time, the composition of the fermentation broth is usually relatively complex, and it is often difficult for amperometric enzyme biosensors to avoid the interference of non-target detection components.
These are the main difficulties that prevent glucose biosensors from being applied to the on-line analysis of industrial fermentation processes.

Method used

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  • Glucose oxidase screen-printed electrode for online detection and preparation method thereof
  • Glucose oxidase screen-printed electrode for online detection and preparation method thereof
  • Glucose oxidase screen-printed electrode for online detection and preparation method thereof

Examples

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Effect test

Embodiment 1

[0037] Detection of Glucose Standards

[0038] 1) Fabrication of Prussian blue-doped mediator-type hydrogen peroxide screen-printed electrodes:

[0039] Preparation containing equimolar concentration of K 3 Fe(CN) 6 And the aqueous solution of KCl, adjust its pH in the scope of 1.0-2.5 with hydrochloric acid, add 1-1.33 times of volumes containing the FeCl of equimolar concentration dropwise to it under rapid stirring condition 3 The aqueous solution is centrifuged at 4000 rpm after fully reacting to obtain the dark blue substance, that is, Prussian blue; it is dried in an oven at 95°C to constant weight, and then baked in the same oven at 100°C-150°C for 0.5-2h to obtain Activated Prussian blue, after cooling in a desiccator, grind it into granules with a mortar for later use; weigh 0.05g of Prussian blue granules, mix it with 1mL N,N-dimethylformamide, grind it in a mortar, then add 3g Conductive carbon paste and 2mL of special solvent for conductive carbon paste are fine...

Embodiment 2

[0056] 1) Fabrication of Prussian blue-doped mediator-type hydrogen peroxide screen-printed electrodes:

[0057] Preparation containing equimolar concentration of K 3 Fe(CN) 6 And the aqueous solution of KCl, adjust its pH in the scope of 1.0-2.5 with hydrochloric acid, add 1-1.33 times of volumes containing the FeCl of equimolar concentration dropwise to it under rapid stirring condition 3 The aqueous solution is centrifuged at 4000 rpm after fully reacting to obtain the dark blue substance, that is, Prussian blue; it is dried in an oven at 95°C to constant weight, and then baked in the same oven at 100°C-150°C for 0.5-2h to obtain Activated Prussian blue, after cooling in a desiccator, grind it into granules with a mortar for later use; weigh 0.05g of Prussian blue granules, mix it with 1mL N,N-dimethylformamide, grind it in a mortar, then add 3g Conductive carbon paste and 2mL of special solvent for conductive carbon paste are finely ground for 20 minutes to fully mix the...

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Abstract

The invention provides a glucose oxidase screen-printed electrode for online detection and a preparation method thereof. According to the electrode, Prussian blue doped carbon-based screen-printed electrode prepared by a chemical deposition method is used as an electrochemical transduction device, and is modified by an immobilized glucose oxidase layer to form an enzyme biosensor. The electrode can be used for effectively avoiding interference caused by complex ingredients in a fermentation broth, and has the characteristics of recycling and good signal reproducibility, so that the requirement for conventional fermentation online analysis can be met. A chemically modified electrode is prepared by adopting a screen-printing process to improve the reproducibility, and can be used for batch production. Furthermore, the enzyme immobilization method used in the electrode is simple, and the aim of effectively limiting enzyme preparation loss can be achieved by using cellulose filter paper and glass cellulose filter paper and other simple materials. The glucose oxidase screen-printed electrode is applicable to modification of enzyme electrode batch production and has enough long enzyme activity life.

Description

technical field [0001] The invention relates to a glucose oxidase screen-printed electrode for on-line monitoring and a preparation method thereof, belonging to the technical field of biosensor and electroanalytical chemical testing. Background technique [0002] A biosensor is a chemical or physical sensor-based transducing device, which can be used to qualitatively or quantitatively analyze a specific substance through the modification of a specific biologically active device. Important areas have a wide range of applications. The amperometric biosensor obtained by enzyme modification has a wide range of applications, a wide variety of raw materials, simple production, reliable sensing performance, convenient and simple use, fast response and time-saving, and is an important part of the biosensor field. [0003] In the industrial production process, it is often necessary to monitor the concentration change of the reaction substrate in the fermentation process in real time...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48C12Q1/00
CPCC12Q1/001G01N27/3277G01N27/48G01N2333/904
Inventor 李一苇史建国杨俊慧孟庆军马耀宏杨艳
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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