A kind of lactase microcapsule and preparation method thereof
A technology of lactase and microcapsules, which is applied in the field of lactase microcapsules and its preparation, can solve the problems of lactase inactivation, poor stability, and incomplete nutrition of products, and achieve gastric protection and good acid stability Effect
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Embodiment 1
[0020] (1) Preparation of lactase glue: Mix κ-carrageenan and sodium alginate at a mass ratio of 8:2 to make a glue, and then dissolve the glue in distilled water at 80°C to prepare a 2.5% mass concentration After the glue is cooled to 43°C, add the lactase solution with a mass concentration of 2% and a temperature of 43°C at a volume ratio of 3:1, and stir with a stirrer at 500r / min for 30min to prepare a lactase glue;
[0021] (2) Ultrasonic defoaming: put the obtained lactase glue in an ultrasonic defoaming machine for ultrasonic defoaming, the ultrasonic frequency is 20kHz, and the intensity is 0.8 W / cm 2 , defoaming time is 2min;
[0022] (3) Spray granulation: Put the defoamed lactase glue into the liquid supply device of the spray granulator, and use an airflow atomizer to flow the lactase glue to the two-fluid nozzle at a flow rate of 250mL / min At the same time, 1.0MPa compressed air is passed into the nozzle to make the lactase glue spray out from the nozzle into tin...
Embodiment 2
[0027] (1) Preparation of lactase glue: Mix κ-carrageenan and sodium alginate at a mass ratio of 8:2 to make a glue, and then dissolve the glue in distilled water at 90°C to prepare a 3.0% mass concentration Glue, after cooling to 46°C, add lactase solution with a mass concentration of 4% and a temperature of 46°C at a volume ratio of 3:1, and stir with a stirrer at 1000 r / min for 20 minutes to prepare a lactase glue;
[0028] (2) Ultrasonic defoaming: put the obtained lactase glue in an ultrasonic defoaming machine for ultrasonic defoaming, the ultrasonic frequency is 30kHz, and the intensity is 0.6 W / cm 2 , defoaming time is 4min;
[0029] (3) Spray granulation: Put the defoamed lactase glue into the liquid supply device of the spray granulator, and use the airflow atomizer to flow the lactase glue to the two-fluid nozzle at a flow rate of 180mL / min At the same time, 0.7MPa compressed air is passed into the nozzle to make the lactase glue spray out from the nozzle into tiny...
Embodiment 3
[0034] (1) Preparation of lactase glue: Mix κ-carrageenan and sodium alginate at a mass ratio of 8:2 to make a glue, and then dissolve the glue in distilled water at 95°C to prepare a 3.5% mass concentration After the glue is cooled to 50°C, add lactase solution with a mass concentration of 6% and a temperature of 50°C at a volume ratio of 3:1, and stir with a stirrer at 1500 r / min for 15 minutes to prepare a lactase glue;
[0035] (2) Ultrasonic defoaming: put the obtained lactase glue in an ultrasonic defoaming machine for ultrasonic defoaming, the ultrasonic frequency is 40kHz, and the intensity is 0.3W / cm 2 , defoaming time is 6min;
[0036](3) Spray granulation: put the defoamed lactase glue into the liquid supply device of the spray granulator, and use an airflow atomizer to flow the lactase glue to the two-fluid nozzle at a flow rate of 100mL / min At the same time, 0.5MPa compressed air is passed into the nozzle to make the lactase glue spray out from the nozzle into ti...
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