Deoxynivalenol aptamer affinity column as well as preparation method and application thereof
A technology of aptamers and affinity columns, applied in chemical instruments and methods, separation methods, preparation of test samples, etc., can solve problems such as poor linearity of standard curves, large coefficient of variation, matrix interference, etc., to reduce Cross-reactivity, high purification efficiency, and high-affinity effects
Inactive Publication Date: 2017-10-20
BEIJING MEIZHENG BIOLOGICAL TECH
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Abstract
The invention relates to a deoxynivalenol aptamer affinity column as well as a preparation method and an application thereof. The affinity purification column adopts a chemical modified solid-phase carrier, a nucleic acid aptamer of deoxynivalenol and the carrier are subjected to covalent coupling, and then the affinity column is filled. The aptamer affinity column is mainly used for purifying deoxynivalenol in food samples, feed samples, milk samples, blood samples and other various samples, so that following HPLC (high performance liquid chromatography) detection and fluorescence detection of deoxynivalenol in the samples can be facilitated.
Application Domain
Other chemical processesPreparing sample for investigation +1
Technology Topic
Solid phasesMilk sample +8
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