Near-infrared second area fluorescence function alloy nanometer point, preparation method and application of near-infrared second area fluorescence function alloy nanometer point in aspect of bioluminescence imaging
An alloy nano- and near-infrared technology, used in fluorescence/phosphorescence, nanotechnology, material analysis by optical means, etc., can solve the problems of low fluorescence efficiency, poor stability, limitations, etc., and achieve good repeatability, easy operation, mild conditions
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Embodiment 1
[0015] In 10 mL of bovine serum albumin (BSA) solution with a concentration of 0.025 mmol / L, add 0.5 mL of AgNO with a concentration of 50 mmol / L in sequence. 3 Solution, 0.01mL of CuSO with a concentration of 25mmol / L 4 Solution, 0.0735g sodium citrate, after heating at 70°C for 5h, centrifuge the reacted solution, add 50mL isopropanol to the clear liquid as a precipitant, collect the precipitate by centrifugation, and disperse the precipitate in 5mL water ; Repeat the operation step of collecting the precipitate by centrifugation and dispersing the precipitate in 5mL water for 3 times, and finally collect the precipitate by centrifugation. The resulting tan precipitate is the Ag / Cu alloy nano dot. Under the transmission electron microscope, the alloy nano The dot size is about 3nm, the particle size is uniform, and the dispersion is good ( figure 1 ).
Embodiment 2
[0017] Weigh 0.15g of polyethylene glycol as a stabilizer and dissolve it in 5mL of water, add 0.25mL of HAuCl with a concentration of 40mmol / L to the solution 4 solution, 0.25mL of CoCl with a concentration of 20mmoL / L 2 Solution, 0.038g sodium borohydride, after heating at 80°C for 4h, centrifuge the reacted solution, add 50mL isopropanol to the clear liquid as a precipitant, collect the precipitate by centrifugation, and disperse the precipitate in 5mL water , repeat the operation steps of collecting the precipitate by centrifugation and dispersing the precipitate in 5mL water for 3 times, and finally collect the precipitate by centrifugation, and the obtained dark yellow precipitate is the Au / Co alloy nanodot. The prepared Au / Co alloy nano-dots have good near-infrared second region fluorescence properties, with an excitation peak of 562nm and a fluorescence emission peak of 1080nm ( figure 2 ).
Embodiment 3
[0019] Preparation of Au / Cu alloy nanodots with different fluorescence emission. Weigh 0.3g of polyethyleneimine and dissolve it in 10mL of water. Add 1 mL of HAuCl at a concentration of 50 mmol / L to the solution 4 solution, or HAuCl with a total volume of 1 mL and a concentration of 50 mmol / L 4 and CuCl 2 Solution, so that the molar ratio of the two is 9.5:0.5, 9:1, 8:2, 7:3, 6:4, 5:5 respectively. Then add 0.3mL hydrazine hydrate to the solution as a reducing agent, react at 100°C for 4h, centrifuge the reacted solution, add 50mL isopropanol to the clear liquid as a precipitant, collect the precipitate by centrifugation, and separate the precipitate Disperse in 5mL of water, repeat the steps of collecting the precipitate by centrifugation, and disperse the precipitate in 5mL of water three times, and finally collect the precipitate by centrifugation to obtain Au / Cu alloy nanodots with adjustable fluorescence. With the increase of copper content in the nanodots, the fluor...
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