Polysaccharide fragment of Dendrobium officinale and extraction method thereof

A technology for polysaccharides and extraction methods of Dendrobium officinale, which is applied in the production and fermentation of bulk chemicals, can solve the problems of low polysaccharide extraction rate, poor polysaccharide quality, and unclear polysaccharide components, and achieve high extraction rate and impurity removal rate. High blood sugar reduction rate is good, and the effect of hypoglycemic effect is remarkable

Active Publication Date: 2017-10-20
连南瑶族自治县欣胜堂生物科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] At present, the research experiments on the hypoglycemic efficacy of Dendrobium officinale all have the disadvantages of low polysaccharide extraction rate, high impurity content, unclear polysaccharide composition, and weak action specificity, and the extraction method is relatively traditional and single, and the quality of the obtained polysaccharide is poor. Nonspecific type of hypoglycemic effect
[0005] "Dendrobium huoshanense polysaccharides and their enzymatic fragments inhibit H 2 o 2 Research on Inducing Apoptosis of Human Lens Epithelial Cells, Bruce Lee, the author of Master's Degree Thesis ...

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  • Polysaccharide fragment of Dendrobium officinale and extraction method thereof
  • Polysaccharide fragment of Dendrobium officinale and extraction method thereof
  • Polysaccharide fragment of Dendrobium officinale and extraction method thereof

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Embodiment 1

[0050] The present embodiment extracts the described Dendrobium officinale polysaccharide fragments through the following steps:

[0051] (1) Water extraction: pulverize Dendrobium officinale to more than 60 meshes, extract by ultrasonic water leaching 3 times, the water used for each extraction is 25 times by weight of Dendrobium officinale, then get the filtrate and carry out decompression concentration to obtain the described Dendrobium officinale. Water extraction solution; the frequency of ultrasonic is 40kHz, and the time of each water extraction is 20min.

[0052] (2) Deproteinization: add 95% ethanol to the water extract of Dendrobium officinale so that the volume fraction of ethanol in the water extract of Dendrobium officinale is 90%, collect the precipitate, dissolve the precipitate in water, add Sevage reagent , shake the solution for 0.5h, then centrifuge at a speed of 1000r / min, and finally take the supernatant to obtain the crude extract, wherein the Sevage reag...

Embodiment 2

[0057] The difference between this embodiment and Embodiment 1 is:

[0058] In the water extraction of the step (1), the number of times of ultrasonic water immersion extraction is 1 time, and the water used for extraction is 20 times by weight of Dendrobium officinale; the frequency of ultrasonic is 30kHz, and the time of each water immersion extraction is 30min.

[0059] In the deproteinization of the step (2), the volume concentration of ethanol added to the water extract of Dendrobium officinale is 90%, so that the volume fraction of ethanol in the water extract of Dendrobium officinale is 80%, and the solution is shaken 0.3% after adding Sevage reagent. h, and then centrifuge at a speed of 900 r / min, and finally take the supernatant;

[0060] In the step (3) enzymatic hydrolysis modification, the enzymatic hydrolysis modification controls the enzymatic hydrolysis temperature to be 48°C, the enzymatic hydrolysis pH value is 4.5, and the enzymatic hydrolysis reaction time i...

Embodiment 3

[0063] The difference between this embodiment and Embodiment 1 is:

[0064] In the water extraction in the step (1), the number of times of ultrasonic water immersion extraction is 2 times, and the water used for extraction is 20 times by weight of Dendrobium officinale; the frequency of ultrasonic is 50kHz, and the time of each water immersion extraction is 10min.

[0065] In the deproteinization of the step (2), the volume concentration of ethanol added to the water extract of Dendrobium officinale is 92%, so that the volume fraction of ethanol in the water extract of Dendrobium officinale is 82%, and the solution is shaken 0.7% after adding Sevage reagent. h, then centrifuge at 1100r / min, and finally take the supernatant;

[0066] In the step (3) enzymatic hydrolysis modification, the enzymatic hydrolysis modification controls the enzymatic hydrolysis temperature to be 50°C, the enzymatic hydrolysis pH value is 5.2, the enzymatic hydrolysis reaction time is 110 min, and the...

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Abstract

The present invention relates to the technical field of Dendrobium officinale, and in particular relates to a polysaccharide fragment of Dendrobium officinale and an extraction method thereof, the extraction methods includes water extraction, deproteinization, enzymatic modification, purification, drying and other steps, the extracted polysaccharide fragment comprises six monosaccharides such as D-glucose, D-mannose, D-galactose, L-rhamnose, D-xylose and D-fucose, experiments prove that the polysaccharide fragment of Dendrobium officinale has non-stimulating effect on insulin secretion of normal mice has higher safety, compared with use of glipizide hypoglycemic drugs, blood glucose decreased rate and glucagon rising rate by use of the polysaccharide fragment of Dendrobium officinale all good, and the polysaccharide fragment of Dendrobium officinale has a significant hypoglycemic effect on a SD rat with streptozotocin induced diabetes.

Description

technical field [0001] The invention relates to the technical field of Dendrobium officinale, in particular to a polysaccharide fragment of Dendrobium officinale and an extraction method thereof. Background technique [0002] Dendrobium officinale Kimura et Migo (Dendrobium officinale Kimura et Migo) is a perennial epiphytic herb of the Orchidaceae. Sweet, slightly cold, it belongs to the liver and kidney meridians. The ancient Chinese medicinal books "Shen Nong's Materia Medica", "Compendium of Materia Medica Supplements", etc. have recorded that fresh or dried stems are used as medicine, which has the effects of nourishing yin and clearing heat, nourishing stomach and promoting body fluid, nourishing kidney and nourishing essence. [0003] Traditional Chinese medicine will have polydipsia, polyphagia, polyuria, long-term body weight loss or urine with sweet taste as the main symptoms, called "diabetes", and diabetes includes diabetes. Diabetes syndrome is characterized b...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02C08B37/00
CPCC08B37/0003C12P19/02C12P19/14Y02P20/54
Inventor 蔡平平卢续贞
Owner 连南瑶族自治县欣胜堂生物科技有限公司
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