Preparation method of trepang compound soft capsules
A technology of soft capsules and sea cucumbers, which is applied in the field of preparation of sea cucumber compound soft capsules, can solve the problems of nutrient loss and reduction of biological activity of sea cucumbers, and achieve the effects of promoting development, realizing high-value utilization, effective preservation and release
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Embodiment 1
[0027] After removing the internal organs of fresh sea cucumbers, homogenize, take 100g of the homogenate, add pH 6.0, 50mM acetic acid buffer to make the substrate concentration reach 15%, and then add 300,000U composite protease. In this example, the subtilisin complex is selected as the composite protease , Product specifications: BR, 200U / mg, vitality: ≥120units / mg. The microwave assisted conditions were 100W, and the reaction temperature was 55℃ for 3h. The sea cucumber enzymatic hydrolysate was inactivated at 95°C for 5 minutes, then concentrated by rotary evaporation at 40°C, freeze-dried, and ground into a powder, which was sieved with a 150-mesh sieve to prepare a microwave-assisted enzymatic hydrolysis sea cucumber product dry powder. Then, according to the mass ratio, the microwave-assisted enzymatic hydrolysis of sea cucumber product dry powder: Chitooligosaccharide: Taurine = 7:1:1 was mixed, and then added to distilled water to make the ratio 1:20, and then mixed ...
Embodiment 2
[0029] After removing the internal organs of fresh sea cucumbers, homogenize, take 100g of the homogenate, add pH 6.5 50mM acetic acid buffer to make the substrate concentration reach 18%, then add 350,000U composite protease, under the microwave assisted condition 200W, reaction temperature is 60℃. Solution 3.5h. The sea cucumber enzymatic hydrolysate was inactivated at 98°C for 8 minutes, then concentrated by rotary evaporation at 45°C, freeze-dried, and ground into a powder, which was sieved with a 180-mesh sieve to prepare a microwave-assisted enzymatic hydrolysis sea cucumber product dry powder. According to the mass ratio, microwave-assisted enzymatic hydrolysis of sea cucumber product dry powder: Chitooligosaccharides: Taurine = 8: 2: 1 to mix, then add it to distilled water to make the ratio 1:25, then stir and mix for spray drying , The uniform microwave-assisted enzymatic hydrolysis of sea cucumber compound is prepared; finally, the microwave-assisted enzymatic hydrol...
Embodiment 3
[0031] After removing the internal organs of fresh sea cucumbers, homogenize, take 100g of the homogenate, add pH 6.5 50mM acetic acid buffer to make the substrate concentration reach 20%, and then add 400,000U composite protease, under microwave assisted conditions of 300W, reaction temperature of 65℃. Solution 4h. The sea cucumber enzymatic hydrolysate was inactivated at 100°C for 10 minutes, then concentrated by rotary evaporation at 50°C, freeze-dried, and ground into a powder, which was sieved with a 200-mesh sieve to prepare a microwave-assisted enzymatic hydrolysis sea cucumber product dry powder. According to the mass ratio, microwave-assisted enzymatic hydrolysis of sea cucumber product dry powder: Chitooligosaccharide: Taurine=9:3:2 was mixed, then added to distilled water to make the ratio 1:30, and then stirred and mixed for spray drying. A uniform microwave-assisted enzymatic hydrolysis of sea cucumber compound is obtained; finally, the microwave-assisted enzymatic...
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