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Edible mushroom enzymolysis process and application thereof for preparing edible mushroom flour

A technology of edible fungus enzyme and edible fungus powder, which is applied in the field of food deep processing, can solve the problems of low utilization of edible fungus nutrients, achieve the effects of accelerating decomposition, simplifying purification steps, and easy control of reaction conditions

Inactive Publication Date: 2017-11-07
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is to provide a kind of edible fungus enzymatic hydrolysis process, to solve the problem of lower utilization of edible fungus nutrients in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The edible fungus enzymatic hydrolysis powder described in this example is obtained by extracting through the following steps:

[0035] (1) Weigh 2000g of fresh edible fungi (lentinus edodes), wash and classify, remove the unfit for consumption such as sediment and bad mushrooms in edible fungi, cut the fresh edible fungi appropriately, and place them evenly in the blast drying In the box, dry at 45°C for 24 hours; the dried samples are crushed by a universal grinder and passed through a 60-mesh sieve to obtain dry edible fungus powder; accurately weigh 100g of edible fungus dry powder, and add a certain amount of Drinking water is used to prepare the edible fungus material liquid for subsequent use;

[0036](2) Preparation of immobilized composite enzyme: Add magnetic hydrotalcite nanoparticles into deionized water and 2mL 0.5% glutaraldehyde solution and stir for cross-linking and activation for 30min, after centrifugation and washing several times, add cellulase (acc...

Embodiment 2

[0040] The edible fungus enzymatic hydrolysis powder described in this example is obtained by extracting through the following steps:

[0041] (1) Weigh 2000g of fresh edible fungi (lentinus edodes), wash and classify, remove the unfit for consumption such as sediment and bad mushrooms in edible fungi, cut the fresh edible fungi appropriately, and place them evenly in the blast drying In the box, dry at 55°C for 20 hours; the dried samples are pulverized by a universal pulverizer and passed through a 60-mesh sieve to obtain dry edible fungus powder; accurately weigh 100g of edible fungus dry powder, and add a certain amount of Drinking water to prepare edible fungus material liquid;

[0042] (2) Preparation of immobilized compound enzyme: get magnetic hydrotalcite nanoparticle and add in deionized water and 2mL 0.5% glutaraldehyde solution and stir cross-linking activation for 30min, after centrifugation and washing several times, add cellulase (accounting for all 0.2%wt% of ...

Embodiment 3

[0046] The edible fungus enzymatic hydrolysis powder described in this embodiment is extracted through the following steps:

[0047] (1) Weigh 2000g of fresh edible fungus (flammulina velutipes), wash and classify, remove the unfit for edible parts such as sediment and bad mushrooms in edible fungi, cut the fresh edible fungi appropriately, and place them evenly in the blast drying In the box, dry at 60°C for 24 hours; the dried samples are crushed by a universal pulverizer and passed through a 60-mesh sieve to obtain dry edible fungus powder; accurately weigh 100g of edible fungus dry powder, and add a certain amount of drinking water to obtain the required edible fungus material liquid;

[0048] (2) Preparation of immobilized compound enzyme: the magnetic Fe 3 o 4 Add deionized water and 2mL of 0.5% glutaraldehyde solution to the nanoparticles and stir for cross-linking activation for 30 minutes, centrifuge and wash several times, add cellulase (accounting for 0.1%wt% of t...

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PUM

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Abstract

The invention belongs to the technical field of food deep processing, in particular relates to an edible mushroom enzymolysis process and further discloses application of the edible mushroom enzymolysis process for preparing edible mushroom flour. The edible mushroom enzymolysis process comprises the following steps: immobilizing cellulase and protease onto a magnetic inorganic material carrier so as to prepare an immobilized complex enzyme, and further performing enzymatic hydrolysis. As an edible mushroom cell wall mainly comprises cellulose, glucan and chitin which are interwoven into a compact reticulate structure which is different from those of other plants, and if edible mushroom is subjected to direct enzymolysis of protease, nutrient substances such as amino acid and nucleotide in cells cannot be completely released. Under the action of synergic effects of the cellulase and the protease for enzymolysis, decomposition and crushing of cell walls are accelerated, the enzymolysis time is shortened, the enzymolysis efficiency is improved, nutrient substances, flavor substances and the like in the edible mushroom can be sufficiently released, and nutrition and feature flavor can be further enriched.

Description

technical field [0001] The invention belongs to the technical field of food deep processing, specifically relates to an enzymatic hydrolysis process of edible fungi, and further discloses its application for preparing edible fungus flour. Background technique [0002] Edible mushrooms refer to mushrooms (large fungi) with huge fruiting bodies and edible mushrooms, commonly known as mushrooms. There are more than 350 kinds of edible fungi known in China, most of which belong to Basidiomycotina. Mushrooms, Ganoderma lucidum, Cordyceps, truffles, Bailing mushrooms and boletus, etc.; a few belong to the Ascomycota subphylum, mainly including morels, saddle mushrooms, and truffles. The above-mentioned fungi grow in different regions and in different ecological environments. [0003] Edible fungi are rich in carbohydrates, proteins, various amino acids, steroids and other components, and have various biological activities such as anti-tumor, anti-oxidation, antibacterial, and li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L7/10A23L29/00A23L31/00
CPCA23L7/198A23L29/06A23L31/00
Inventor 崔文甲王文亮王月明弓志青王延圣贾凤娟沈文凤
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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