Edible mushroom enzymolysis process and application thereof for preparing edible mushroom flour
A technology of edible fungus enzyme and edible fungus powder, which is applied in the field of food deep processing, can solve the problems of low utilization of edible fungus nutrients, achieve the effects of accelerating decomposition, simplifying purification steps, and easy control of reaction conditions
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Embodiment 1
[0034] The edible fungus enzymatic hydrolysis powder described in this example is obtained by extracting through the following steps:
[0035] (1) Weigh 2000g of fresh edible fungi (lentinus edodes), wash and classify, remove the unfit for consumption such as sediment and bad mushrooms in edible fungi, cut the fresh edible fungi appropriately, and place them evenly in the blast drying In the box, dry at 45°C for 24 hours; the dried samples are crushed by a universal grinder and passed through a 60-mesh sieve to obtain dry edible fungus powder; accurately weigh 100g of edible fungus dry powder, and add a certain amount of Drinking water is used to prepare the edible fungus material liquid for subsequent use;
[0036](2) Preparation of immobilized composite enzyme: Add magnetic hydrotalcite nanoparticles into deionized water and 2mL 0.5% glutaraldehyde solution and stir for cross-linking and activation for 30min, after centrifugation and washing several times, add cellulase (acc...
Embodiment 2
[0040] The edible fungus enzymatic hydrolysis powder described in this example is obtained by extracting through the following steps:
[0041] (1) Weigh 2000g of fresh edible fungi (lentinus edodes), wash and classify, remove the unfit for consumption such as sediment and bad mushrooms in edible fungi, cut the fresh edible fungi appropriately, and place them evenly in the blast drying In the box, dry at 55°C for 20 hours; the dried samples are pulverized by a universal pulverizer and passed through a 60-mesh sieve to obtain dry edible fungus powder; accurately weigh 100g of edible fungus dry powder, and add a certain amount of Drinking water to prepare edible fungus material liquid;
[0042] (2) Preparation of immobilized compound enzyme: get magnetic hydrotalcite nanoparticle and add in deionized water and 2mL 0.5% glutaraldehyde solution and stir cross-linking activation for 30min, after centrifugation and washing several times, add cellulase (accounting for all 0.2%wt% of ...
Embodiment 3
[0046] The edible fungus enzymatic hydrolysis powder described in this embodiment is extracted through the following steps:
[0047] (1) Weigh 2000g of fresh edible fungus (flammulina velutipes), wash and classify, remove the unfit for edible parts such as sediment and bad mushrooms in edible fungi, cut the fresh edible fungi appropriately, and place them evenly in the blast drying In the box, dry at 60°C for 24 hours; the dried samples are crushed by a universal pulverizer and passed through a 60-mesh sieve to obtain dry edible fungus powder; accurately weigh 100g of edible fungus dry powder, and add a certain amount of drinking water to obtain the required edible fungus material liquid;
[0048] (2) Preparation of immobilized compound enzyme: the magnetic Fe 3 o 4 Add deionized water and 2mL of 0.5% glutaraldehyde solution to the nanoparticles and stir for cross-linking activation for 30 minutes, centrifuge and wash several times, add cellulase (accounting for 0.1%wt% of t...
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