A kind of anti-chicken coccidia probiotic drug and its preparation method and application
A technology for chicken coccidiosis and chicken coccidiosis, which is applied in the field of anti-chicken coccidiosis probiotics and its preparation, can solve the problems of difficulty in extraction and separation, low extraction rate of effective active components, etc., and achieves high extraction efficiency, enhanced immunity, The effect of controlling coccidiosis in chicks
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Embodiment 1
[0022] The preparation of embodiment 1 active yeast culture, the steps are as follows:
[0023] (1) Preparation of medium:
[0024] Liquid medium: the ingredients are: glucose 50 g / L, yeast extract 2.0 g / L, wort 120 mL / L, MgSO 4 0.5 g / L, FeSO 4 0.1 g / L, urea 1.0 g / L. Adjust the pH to 6.0, sterilize at 121°C for 20 min, dispense into Erlenmeyer flasks, and set aside.
[0025] Slant and plate medium: the ingredients are: glucose 50 g / L, yeast extract 2.0 g / L, wort 120 mL / L, MgSO 4 0.5 g / L, FeSO 4 0.1 g / L, urea 1.0 g / L, agar 20 g / L. Adjust the pH to 6.0, sterilize at 121°C for 20 minutes, dispense into Erlenmeyer flasks, and set aside.
[0026] (2) Yeast culture:
[0027] Saccharomyces cerevisiae( Saccharomyces cerevisiae , purchased from China Veterinary Microbiological Strain Collection Management Center, feed yeast, strain number CVCC 2.1011), pick out a relatively plump yeast lawn from the slant storage, and inoculate it in 50 mL of liquid culture culture medium ...
Embodiment 2
[0029] Embodiment 2 The preparation method of Chinese medicine compound extract, wherein the proportioning of each single Chinese medicine is as follows:
[0030]The traditional Chinese medicine compound extract is composed of the following components by weight: 60-90 parts of Changshan, 30-60 parts of Tsaoguo, 40-60 parts of Dijincao, 30-50 parts of purslane, 20-40 parts of amaranth, 10-30 parts of black leek, 10-30 parts of licorice, 20-40 parts of black plum, 20-50 parts of tangerine peel, 10-30 parts of divine comedy.
[0031] The preparation method of the Chinese medicine compound extract is as follows: pulverize each single Chinese medicine through an ultrafine pulverizer, weigh the single Chinese medicine according to the formula amount, add 6-10 times the amount of deionized water of the total weight of the Chinese medicine, soak for 30- 60 minutes, decoct for 40-60 minutes, filter to remove residue; add 3-5 times the amount of deionized water to the filter residue, de...
Embodiment 3
[0046] Embodiment 3 The preparation method of fermentation filtrate, the steps are as follows:
[0047] Taking the combined decoction filtrate prepared in Example 2 as a unit, add maltose 200-300 g / L, wort juice 100-200 mL / L, MgSO 4 5.0 g / L, FeSO 4 3.0 g / L. Sterilize at 121°C for 15 minutes. Cool to about 30°C, inoculate 80-100 mL / L filtrate of the active Saccharomyces cerevisiae culture prepared in Example 1 (the inoculum size is 8-10%, and the percentage is volume percentage), at 35°C, facultative anaerobic fermentation culture for 7-10 Days (the sealing film of the mouth of the fermentation bottle has air holes, not completely sealed, placed in a sterile fermentation room, and observed on a shaking table at 40 r / min), until a white bacterial film grows on the liquid surface.
[0048] The following are several optimal fermentation filtrate preparation methods, and the combined decoction filtrate used is preferably Chinese medicine compound extract No. 1:
[0049] (1) Fer...
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