Unlock instant, AI-driven research and patent intelligence for your innovation.

Compound Clostridium sporogenes spore inhibitor

A technology of Clostridium sporogenes and inhibitors, which is applied in the field of compound Clostridium sporogenes spore inhibitors, can solve the problem of no natural preservatives and the like

Active Publication Date: 2020-03-27
QILU UNIV OF TECH
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to make up for the deficiencies of the prior art and solve the problem that there is no natural preservative that can effectively replace nitrite in the prior art to inhibit the germination of Clostridium sporogenes spores, the invention provides a compound Clostridium sporogenes spore inhibitor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound Clostridium sporogenes spore inhibitor
  • Compound Clostridium sporogenes spore inhibitor
  • Compound Clostridium sporogenes spore inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1.1 Clostridium sporogenes spore preparation

[0027] The activated Clostridium sporogenes strains were inserted into sterile spore medium, cultured at 30°C for 14 days, and then cultured at 20°C for 7 days. After the culture is over, centrifuge at 8000r / min for 5min, remove the supernatant, keep the sediment, and wash with sterile distilled water. Centrifuge, wash and repeat 5-6 times under the above conditions, and finally dilute the sediment with PBS buffer solution to obtain a spore liquid. Place the spore liquid in a water bath at 95°C for 15 minutes, and cool it rapidly to prepare the Clostridium sporogenes spore liquid, and store it in a refrigerator at 2-8°C until use. Morphological characteristics of Clostridium sporogenes spores figure 1 shown.

[0028] Depend on figure 1 It can be seen that the diameter of the dyed Clostridium sporogenes spores is obviously larger than that of the thallus, and it is located in the middle or at one end of the thallus of Cl...

Embodiment 2

[0068] The purchased fresh pork was cut into uniform small pieces (1×1×1 cm) with sterilized knives, and divided into 3 groups on average, and inoculated with Clostridium sporogenes spores. Dip the meat pieces in each group in sterilized distilled water containing monascus pigment, compound antibacterial agent, NaNO 2 in the solution for 5 minutes, then filter it with a sterile stainless steel mesh sieve for 1 minute, and finally put all the meat pieces into sterile plastic bags and store them in a special refrigerator at low temperature (4°C). Samples were taken from each bag and minced using a meat grinder to determine sensory quality, pH, TVB-N and TBC.

[0069] 2.1 Sensory observation

[0070] According to GB5009.44-2003 "Meat and Meat Products Hygienic Standard Analysis Method", the freshness of the sample is judged by observing and comparing the color, viscosity, smell, etc. of the sample.

[0071] Add different antibacterial agents to the samples and store them at 4°C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a compound clostridium sporogene inhibitor, and belongs to the field of antibacterial agents. The compound clostridium sporogene inhibitor is mixed solution of 0.007-0.020 wt% of sigma-lysine hydrochloride, 0.018-0.030 wt% of potassium cinnamate, 0.058-0.073 wt% of chitosan and 0.040-0.051 wt% GML (glycerol monolaurate). The inhibitor has obvious synergism in inhibition for germination of clostridium sporogene by compounding sigma-lysine hydrochloride, potassium cinnamate, chitosan and GML. The natural compound inhibitor disclosed by the invention achieves and even exceeds the effect of nitrite in inhibition for the germination of the clostridium sporogene and the natural compound inhibitor can completely replace nitrite to be applied in storage of meat products.

Description

technical field [0001] The invention relates to the field of antibacterial agents, in particular to a compound Clostridium sporogenes spore inhibitor. Background technique [0002] In recent years, with the widespread rise and use of food additives, while the problem of food corruption has been initially resolved, some additives will also pose a threat to human health, such as nitrite. Therefore, the use of natural food additives to completely replace or partially replace synthetic additives has attracted attention. The natural preservatives that have been developed and applied are mainly divided into three categories: vegetable, animal and microbial. Scholars such as Vermeiren used lactic acid bacteria Lactobacillus sakei subsp.Carnosus (10A) in the research on the preservation of vacuum-packed steamed ham, and found that it can inhibit Leuconostoc and Pseudomonas chlorophyll; Ekwenye et al found that garlic And Ginkgo biloba extract has an effective inhibitory effect on ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N55/02A01N33/08A23L3/3562A23L3/3499
CPCA23L3/3508A23L3/3517A23L3/3526A23L3/3562A23V2002/00A23V2250/511
Inventor 张志国
Owner QILU UNIV OF TECH