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Preparation method of magnetic double targeting nano-particles for cancer treatment and application

A nanoparticle, dual-targeting technology, applied in the field of biomedical materials, can solve problems such as inability to reach inside cells, human side effects, tumor cell drug resistance, etc., achieve growth inhibition of blood vessels around cells, and good biocompatibility , the effect of slow growth of cancer cells

Inactive Publication Date: 2017-11-24
SHANGHAI LINKCHEM TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term chemotherapy will cause serious drug resistance in tumor cells, so that the desired effect cannot be achieved.
Although radiotherapy and chemotherapy kill tumor cells to a certain extent, they also cause damage to normal cells in the human body and cause side effects in the human body
[0003] Traditional cancer drugs are often swallowed up by phagocytes in the human immune system, making the drugs ineffective
What is more difficult to handle is that the traditional drug molecules are large in size and cannot reach the inside of the cell

Method used

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  • Preparation method of magnetic double targeting nano-particles for cancer treatment and application
  • Preparation method of magnetic double targeting nano-particles for cancer treatment and application
  • Preparation method of magnetic double targeting nano-particles for cancer treatment and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Fe 3 o 4 Preparation of MNPs stock solution

[0034] Formulated with 2M FeCl 2 4H 2 O, 1M FeCl 3 ·6H 2 A mixed solution of O and 2M HCl was deoxygenated with nitrogen for 10 min. in N 2 Under certain conditions, the mixed iron salt solution was added dropwise into 250mL of 0.7M anaerobic ammonia water, and vigorously stirred at room temperature for 30min. Separation by magnet, washing with ultrapure water to neutrality, constant volume to 250mL volumetric flask, sealed storage to obtain Fe 3 o 4 MNPs stock solution.

Embodiment 2

[0036] Preparation of magnetic Prussian blue-like nanoparticles with a molar ratio of iron and zinc of 3:1:

[0037] Accurately prepare volumetric flask containing 1.125mM FeSO 4 and 0.375mM ZnCl 2 100mL of the mixed solution, and weighed 1000mg of polyvinylpyrrolidone (PVP) and an equal amount of hyaluronic acid with an analytical balance and added to the mixed solution.

[0038] Use a volumetric flask to accurately prepare 50 mL of K containing 1.5 mM 4 [Fe(CN) 6 ] solution.

[0039] Pour the prepared two solutions into two 250mL beakers respectively, and add Fe 3 o 4 The MNPs stock solution constituted a mixed solution, and then the mixed solution was added dropwise to the K 4 [Fe(CN) 6 ] solution.

[0040] After stirring with a magnetic stirrer at room temperature for 3 h, the mixed solution was dialyzed in a dialysis bag containing distilled water for two days.

[0041] The solid matter was pulled out, put into a freeze dryer, and the solid product was collected ...

Embodiment 3

[0043] The as-prepared magnetic Zn-Fe PB NPH, using AA test, targeted Cu removal for magnetic Zn-Fe PB NPH 2+ Ability to test, the test results are as follows:

[0044] (1) Using the AA test method, magnetic Zn-Fe PB NPH on Cu 2+ , Mn 2+ , Mg 2+ and Ca 2+ The ion removal ability was characterized. Test results such as figure 1 As shown, it can be seen from the table that the magnetic Zn-Fe PB NPH has the strongest ability to remove copper ions, indicating that the selective copper reduction is not high for other elements, and the effect is very good.

[0045] (2) Using the AA test method, the magnetic Zn-Fe PB NPH effect on Cu 2+ The removal rate was characterized. Test results such as figure 2 , it can be seen from the table that before 1080 minutes, Cu 2+ The removal rate increases gradually, and the increasing speed gradually slows down. After 1080 minutes, Cu 2+ The remaining ratio remains unchanged, indicating that the magnetic Zn-Fe PB NPH has a positive effe...

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Abstract

The invention discloses a preparation method of magnetic double targeting nano-particles for cancer treatment and an application. The nano-particles are formed by adopting the nano-particles doped with Fe3O4 as a core and prussian blue-like nano-particles as a shell structure; the surfaces of the nano-particles are coated with a surface protectant; the surface protectant is a mixture of polyvinylpyrrolidone and a hyaluronic acid or a mixture of chitosan and the hyaluronic acid. The nano-particles can be effectively subjected to magnetic targeting distribution under the action of an external magnetic field to be swallowed by cells, Cu<2+> is selectively swapped out to be used as stably and slowly released drug for treating cancers. The core-shell structure surface of the nano-particles is coated with the polyvinylpyrrolidone and the hyaluronic acid / chitosan, so that the inside is prevented from being affected by the physiological environment and the magnetic double targeting nano-particles have good biocompatibility.

Description

technical field [0001] The invention relates to a kind of magnetic Prussian blue nanoparticle (Fe 3 o 4 MN / Zn-Fe (PB PH) preparation method and application thereof. Background technique [0002] Cancer is a general term for various malignant tumors, and it is a major problem facing mankind in the world today. Cancer is generally difficult to cure, and the number of people dying of cancer in the world is increasing every year. Not only the patients are under great mental pressure, but also the family members are under great mental and material pressure. Traditional treatments include surgery, radiation and chemotherapy. For traditional surgery, well-developed early tumors can be removed, but the disadvantage is that the tumor may not be completely removed, and the possibility of recurrence is extremely high. Radiation therapy uses high-energy rays to kill tumors to achieve the purpose of treatment, but it may greatly mutate tumor cells and cause great damage to normal cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/36A61K47/32A61K33/26A61P35/00
Inventor 陆茜白国涛
Owner SHANGHAI LINKCHEM TECH CO LTD
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